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EC 1.14.13.96 Details
show Brenda entryEC number
1.14.13.96
Accepted name
5β-cholestane-3α,7α-diol 12α-hydroxylase
Reaction
5β-cholestane-3α,7α-diol + NADPH + H+ + O2 = 5β-cholestane-3α,7α,12α-triol + NADP+ + H2O
Other name(s)
5β-cholestane-3α,7α-diol 12α-monooxygenase,
sterol 12α-hydroxylase (ambiguous),
CYP8B1,
cytochrome P450 8B1
Systematic name
5β-cholestane-3α,7α-diol,NADPH:oxygen oxidoreductase (12α-hydroxylating)
Comment
A heme-thiolate protein (P-450). This is the key enzyme in the biosynthesis of the bile acid cholic acid (3α,7α,12α-trihydroxy-5β-cholanoic acid). The activity of this enzyme determines the biosynthetic ratio between cholic acid and chenodeoxycholic acid [3]. The enzyme can also hydroxylate the substrate at the 25 and 26 position, but to a lesser extent [1].
History
created 2005, deleted 2018
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1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.6.4.1 created 1961, deleted 2002 1.6.4.2 created 1961, modified 1989, deleted 2002 1.6.4.3 created 1961, modified 1976, deleted 1983 1.6.4.4 created 1965, deleted 2002 1.6.4.5 created 1972, deleted 2002 1.6.4.6 created 1972, deleted 2002 1.6.4.7 created 1978, deleted 2002 1.6.4.8 created 1989, deleted 2002 1.6.4.9 created 1992, deleted 2002 1.6.4.10 created 1992, deleted 2002 1.14.13.45 created 1992, deleted 2003
