EC Explorer

1.14.13.88

flavanoid 3′,5′-hydroxylase

a flavanone + 2 NADPH + 2 H⁺ + 2 O₂ = a 3′,5′-dihydroxyflavanone + 2 NADP⁺ + 2 H₂O (overall reaction);;(1a) a flavanone + NADPH + H⁺ + O₂ = a 3′-hydroxyflavanone + NADP⁺ + H₂O;;(1b) a 3′-hydroxyflavanone + NADPH + H⁺ + O₂ = a 3′,5′-dihydroxyflavanone + NADP⁺ + H₂O

flavonoid 3′,5′-hydroxylase

flavanone,NADPH:oxygen oxidoreductase

94047-23-1

A heme-thiolate protein (P-450). The 3′,5′-dihydroxyflavanone is formed via the 3′-hydroxyflavanone. In Petunia hybrida the enzyme acts on naringenin, eriodictyol, dihydroquercetin (taxifolin) and dihydrokaempferol (aromadendrin). The enzyme catalyses the hydroxylation of 5,7,4′-trihydroxyflavanone (naringenin) at either the 3′ position to form eriodictyol or at both the 3′ and 5′ positions to form 5,7,3′,4′,5′-pentahydroxyflavanone (dihydrotricetin). The enzyme also catalyses the hydroxylation of 3,5,7,3′,4′-pentahydroxyflavanone (taxifolin) at the 5′ position, forming ampelopsin. NADH is not a good substitute for NADPH.

created 2004, deleted 2018