2.7.1.21: thymidine kinase
This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.
Word Map on EC 2.7.1.21
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2.7.1.21
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herpes
-
simplex
-
ganciclovir
-
suicide
-
nucleoside
-
hsv-tk
-
acyclovir
-
viruses
-
triphosphate
-
adenovirus
-
prodrugs
-
salvage
-
lymphocyte
-
vaccinia
-
herpesvirus
-
pyrimidine
-
bystander
-
retroviral
-
chloramphenicol
-
positron
-
glioma
-
intratumoral
-
cotransfection
-
deoxycytidine
-
varicella-zoster
-
cytomegalovirus
-
tomography
-
nude
-
lymphoma
-
dna-mediated
-
dtmp
-
deoxynucleoside
-
retrovirus-mediated
-
oncolytic
-
adenovirus-mediated
-
gene-modified
-
deoxyguanosine
-
deoxyribonucleoside
-
synthesis
-
medicine
-
herpetic
-
diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
-
zidovudine
-
kinase-deficient
-
molecular biology
-
3'-azido-3'-deoxythymidine
-
pseudorabies
-
replication-competent
-
varicella
-
zoster
-
5-fluorocytosine
- 2.7.1.21
- herpes
- simplex
- ganciclovir
-
suicide
- nucleoside
-
hsv-tk
- acyclovir
- viruses
- triphosphate
- adenovirus
-
prodrugs
-
salvage
- lymphocyte
- vaccinia
- herpesvirus
- pyrimidine
-
bystander
-
retroviral
- chloramphenicol
-
positron
- glioma
-
intratumoral
-
cotransfection
- deoxycytidine
-
varicella-zoster
- cytomegalovirus
-
tomography
-
nude
- lymphoma
-
dna-mediated
- dtmp
- deoxynucleoside
-
retrovirus-mediated
-
oncolytic
-
adenovirus-mediated
-
gene-modified
- deoxyguanosine
- deoxyribonucleoside
- synthesis
- medicine
-
herpetic
- diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
- zidovudine
-
kinase-deficient
- molecular biology
- 3'-azido-3'-deoxythymidine
- pseudorabies
-
replication-competent
- varicella
- zoster
- 5-fluorocytosine
Reaction
Synonyms
2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK
ECTree
2.7.1.21 thymidine kinaseAdvanced search results
results (
results (KM Value
KM Value on EC 2.7.1.21 - thymidine kinase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.075
2',3'-dideoxy-2',3'-didehydrothymidine
37°C, pH not specified in the publication
0.03
2',3'-dideoxythymidine
37°C, pH not specified in the publication
0.132
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil
37°C, pH not specified in the publication
0.0563
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0174
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0318
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0356
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0552
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0799
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0373
3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0552
3-[1(2)-propyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.114
dioxolane thymidine
37°C, pH not specified in the publication
0.00065
2',3'-dideoxy-3'-azidothymidine
37°C, pH not specified in the publication
0.0087
2',3'-dideoxy-3'-azidothymidine
82°C, pH not specified in the publication
0.0065
2'-deoxythymidine
-
dNTP-free recombinant tetrameric enzyme, pH 7.5, 25°C
0.00038
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.00043
3'-azido-3'-deoxythymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.00052
3'-azido-3'-deoxythymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0027
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.012
3'-azido-3'-deoxythymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.018
3'-azido-3'-deoxythymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.033
3'-azido-3'-deoxythymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.204
3'-azido-3'-deoxythymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.254
3'-azido-3'-deoxythymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.406
3'-azido-3'-deoxythymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
2.318
3'-azido-3'-deoxythymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.001
5-fluoro-2'-deoxyuridine
37°C, pH not specified in the publication
0.098
ATP
-
thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.18
ATP
-
full-length enzyme, with 0.0005 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.21
ATP
-
full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.22
ATP
-
full-length enzyme, with 0.005 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.32
ATP
-
full-length enzyme, with 0.5 mM deoxyinosine as cosubstrate, at pH 7.8 and 37°C
0.0036
deoxycytidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0066
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.015
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.00023
thymidine
P03176
mutant enzyme P12N/H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.00027
thymidine
at 37°C for 20 min in 30 mM potassium phosphate, pH 8.0, 1.2 mM MgCl2
0.0003
thymidine
-
pH 7.5, recombinant mutants V106A and V106G, in presence of ATP
0.0003
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0004
thymidine
-
pH 7.5, recombinant mutants V106L and V106G, in absence of ATP
0.0004
thymidine
-
pH 7.5, recombinant mutants V106L, V106H and V106T, in presence of ATP
0.00044
thymidine
P03176
wild type enzyme, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0005
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
-
pH 7.5, recombinant mutant V106M, in absence or presence of ATP
0.0006
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0006
thymidine
Q9S750; Q9FN47
subunit alpha, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.00062
thymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.0007
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0007
thymidine
Q9S750; Q9FN47
subunit alpha, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0008
thymidine
-
pH 7.5, recombinant mutant V106Q, in absence or presence of ATP
0.0009
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0009
thymidine
Q9S750; Q9FN47
subunit beta, in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0009
thymidine
Q9S750; Q9FN47
subunit beta, in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0012
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0013
thymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0014
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0014
thymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.0014
thymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0016
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.002
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0023
thymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.0024
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0025
thymidine
in the presence of 2.5 mM ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.0039
thymidine
P03176
mutant enzyme P12N, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0039
thymidine
-
thymidine kinase-2 mutant enzyme I59Y, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0043
thymidine
-
wild type thymidine kinase-2, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0049
thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.0051
thymidine
-
thymidine kinase-2 mutant enzyme I59V, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.0056
thymidine
-
thymidine kinase-2 mutant enzyme I59L, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.006
thymidine
-
thymidine kinase-2 mutant enzyme I59H, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.008
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.012
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.016
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.017
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.017
thymidine
in the absence of ATP, in 50 mM Tris/HCl (pH 8.0), 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, temperature not specified in the publication
0.036
thymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.037
thymidine
P03176
mutant enzyme H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.059
thymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.074
thymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.263
thymidine
P03176
mutant enzyme P12N/H13I/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.317
thymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.336
thymidine
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.349
thymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.565
thymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.59
thymidine
P03176
mutant enzyme P12N/Y127F, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
0.612
thymidine
P03176
mutant enzyme H13I, in 50 mM Tris/HCl, pH 8.0, 2.5 mM MgCl2, 10 mM dithiothreitol, 0.5 mM CHAPS, 3 mg/ml bovine serum albumin, at 37°C
additional information
additional information
-
kinetics of wild-type and mutant enzymes in absence or presence of ATP, overview
-
additional information
additional information
-
kinetics, ATP-binding kinetics of wild-type and mutant enzymes at room temperature
-
additional information
additional information
-
steady-state kinetics, kinetics of conformational changes induced by ligand binding
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