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2.7.1.21: thymidine kinase

This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.

Word Map on EC 2.7.1.21

Reaction

ATP
+
thymidine
=
ADP
 +
dTMP

Synonyms

2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.21 thymidine kinase

General Stability

General Stability on EC 2.7.1.21 - thymidine kinase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol protects against thermal inactivation
Herpes simplex virus
-
ATP or thymidine stabilizes, type I more stable in the presence of MgATP2- than type II
Herpes simplex virus
-
binding of dNTP inhibitors and substrates, i.e. 2'-deoxycytidine, Mg-2'-deoxyCTP, 2'-deoxythymidine, and Mg-2'-deoxyTTP structurally stabilizes the enzyme, ligand-bound enzyme is less susceptible to aggregation, proteolysis by trypsin, and thermal denaturation
-
bovine serum albumin, 0.5 mg/ml stabilizes
-
digitonin stabilizes
-
dithiothreitol essential for stability
-
dithiothreitol, 5 mM stabilizes
-
dithiothreitol, ATP and other nucleosides stabilize
-
dTTP protects against thermal inactivation
Tequatrovirus T4
-
not stabilized by 10% glycerol, 5 mM 2-mercaptoethanol, 5 mM EDTA, 1 mg/ml bovine serum albumin
-
stability decreases with increasing purity, ATP, thymidine, Mg2+ protect
-
stable in the presence of ATP, bovine serum albumin and CHAPS
-