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2.7.1.21: thymidine kinase

This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.

Word Map on EC 2.7.1.21

Reaction

ATP
+
thymidine
=
ADP
+
dTMP

Synonyms

2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.21 thymidine kinase

Cloned

Cloned on EC 2.7.1.21 - thymidine kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination, expression in Escherichia coli as maltose binding fusion protein in inclusion bodies, coexpression of GroEL/ES molecular chaperone system and 3% v/v ethanol supplementation
-
DNA and amino acid sequence determination, expression in Escherichia coli as maltose binding fusion protein in inclusion bodies, coexpression of GroEL/ES molecular chaperone system improves enzyme solubility only marginally, but ethanol supplementation contributes to enzyme recovery due to activation of heat shock response
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3) cells
-
expressed in Escherichia coli ER2566 cells
-
expressed in Escherichia coli KY895 cells
expressed in Escherichia coli Rosetta(DE3) cells as a fusion protein to glutathione transferase
-
expression as GST-tagged enzyme in Escherichia coli strain BL21
-
expression in Escherichia coli
expression in Escherichia coli as poly-histidine fusion protein in the pET-16b vector
expression of C-terminally truncated enzyme lacking 41 amino acid residues
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
expression of the His-tagged, thrombin-cleavable full length or truncated enzyme in Escherichia coli strain BL21 C41 (DE3)
Herpes simplex virus type 4
-
expression of wild-type and Val106 mutants in an enzyme-deficient Escherichia coli strain as GST-fusion proteins
-
gene tdk, expression in Escherichia coli as wild-type and selenomethionine-labeled enzymes
-
gene thyB, DNA and amino acid sequence determination and analysis, phage display, location on chromosome 5, 3 exons, 2 introns, expression in Escherichia coli strain BL21
glutathione S-transferase fusion protein
heterologously overexpressed in Escherichia coli
N-terminal thrombin-cleavable His6-tagged enzyme, missing 14 amino acids of the N-terminus and 40 amino acids of the C-terminus, in Escherichia coli
overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain B834(DE3), transient expression as Flag-tagged enzyme in LM-tk- cells
-
PreScission-cleavable glutathione S-transferase fusion protein
-