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Literature summary for 2.7.1.21 extracted from

  • Sherley, J.L.; Kelly, T.J.
    Human cytosolic thymidine kinase. Purification and physical characterization of the enzyme from HeLa cells (1988), J. Biol. Chem., 263, 375-382.
    View publication on PubMed
Search for more literature for 2.7.1.21

General Stability

General Stability Organism
digitonin stabilizes Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
 2 * 24000, cytosol, SDS-PAGE Homo sapiens
84000 98000 calculation from Stokes' radius and sedimentation coefficient Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + thymidine Homo sapiens involved in DNA replication ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
rLyTK1Val-106 and rLyTK1Met-106 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
325
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 2 weeks, no loss of activity in presence of digitonin Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thymidine
-
 Homo sapiens ADP + dTMP
-
 ?
ATP + thymidine involved in DNA replication Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 24000, cytosol, SDS-PAGE Homo sapiens