Any feedback?
Literature summary for 2.7.1.21 extracted from
- Thymidine kinase from blast cells of myelocytic leukemia (1978), Methods Enzymol., 51, 365-371.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin, 0.5 mg/ml stabilizes | Homo sapiens |
| dithiothreitol essential for stability | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| dCTP | mitochondrial enzyme | Homo sapiens |  |
| dTTP | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
thymidine | cytoplasmic enzyme, pH 7.5, 37°C | Homo sapiens | |
| 0.003 | - |
deoxycytidine | mitochondrial enzyme, pH 7.5, 37°C | Homo sapiens | |
| 0.005 | 0.0052 | thymidine | mitochondrial enzyme, pH 7.5, 37°C | Homo sapiens | |
| 0.1 | - |
ATP | mitochondrial enzyme, pH 7.5, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Cu2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Fe2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Mg2+ | - |
Homo sapiens | |
| Mn2+ | can partially substitute for Mg2+ | Homo sapiens | |
| Zn2+ | can partially substitute for Mg2+ | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | 74000 | mitochondria, glycerol gradient centrifugation | Homo sapiens |
| 84000 | 98000 | cytoplasm, glycerol density gradient centrifugation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| rLyTK1Val-106 and rLyTK1Met-106 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blast cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
| ATP + 5-bromo-2'-deoxyuridine | - |
Homo sapiens | ADP + 5-bromo-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + 5-iodo-2'-deoxyuridine | - |
Homo sapiens | ADP + 5-iodo-2'-deoxyuridine 5'-phosphate | - |
? | |
| ATP + thymidine | ATP can be substituted by dATP | Homo sapiens | ADP + dTMP | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
dCTP | pH 7.5, 37°C | Homo sapiens | |
| 0.01 | - |
dTTP | pH 7.5, 37°C | Homo sapiens | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
