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2.7.1.21: thymidine kinase

This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.

Word Map on EC 2.7.1.21

Reaction

ATP
+
thymidine
=
ADP
 +
dTMP

Synonyms

2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.21 thymidine kinase

Temperature Stability

Temperature Stability on EC 2.7.1.21 - thymidine kinase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
-
rLyTK1Met-106: 50% loss of activity within 41 min, rLyTK1Val-106: 50% loss of activity after 392 min
20
-
deletion of the 20 C-terminal amino acids decreases half-life at 20°C from 83 min for wild-type enzyme to 78 min, deletion of the 40 C-terninal amino acids increases half-life to 335 min, deletion of the 44 C-terminal amino acids increases half-life to 500 min
25 - 40
-
40 min stable
27 - 43
Herpes simplex virus
-
stable
37
-
20% loss of activity in the presence of bovine serum albumin, thymidine and dithiothreitol after 60 min, almost complete loss of activity witout bovine serum albumin, thymidine and dithiothreitol after 60 min
50
Tequatrovirus T4
-
quick inactivation above, dTTP protects against thermal inactivation
additional information