Literature summary for 2.7.1.21 extracted from

Zhu, C.; Harlow, L.S.; Berenstein, D.; Munch-Petersen, S.; Munch-Petersen, B.
Effect of C-terminal of human cytosolic thymidine kinase (TK1) on in vitro stability and enzymatic properties (2006), Nucleosides Nucleotides Nucleic Acids, 25, 1185-1188.

Search for more literature for 2.7.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of the 20 C-terminal amino acids decreases half-life at 20°C from 83 min for wild-type enzyme to 78 min, deletion of the 40 C-terninal amino acids increases half-life to 335 min, deletion of the 44 C-terminal amino acids increases half-life to 500 min	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + methylthymidine	-	Homo sapiens	ADP + methylthymidine 5'-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
TK1	-	Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
20	-	deletion of the 20 C-terminal amino acids decreases half-life at 20°C from 83 min for wild-type enzyme to 78 min, deletion of the 40 C-terninal amino acids increases half-life to 335 min, deletion of the 44 C-terminal amino acids increases half-life to 500 min	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	-	Homo sapiens

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Release 2023.1 (January 2023)