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2.7.1.21: thymidine kinase

This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.

Word Map on EC 2.7.1.21

Reaction

ATP
+
thymidine
=
ADP
+
dTMP

Synonyms

2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.21 thymidine kinase

Storage Stability

Storage Stability on EC 2.7.1.21 - thymidine kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.1 mM thymidine, 1 week, 30% loss of activity
-
-20°C, 50 mM Tris-HCl buffer, pH 8.0, 10 mM 2-mercaptoethanol, 20% glycerol, 100 mM KCl, 6 months, 20% loss of activity
-
-20°C, 50% glycerol, relatively stable
-
-20°C, enzyme in buffer containing 250 mM imidazole, several months, without noticeable loss of activity
-
-20°C, or 4°C, 24 h, more than 50% loss of activity
-
-70°C, 0.2 mM deoxythymidine, 3 mM dithiothreitol, 10% glycerol
Herpes simplex virus
-
-70°C, 10 mM 2-mercaptoethanol, 0.5 mg/ml bovine serum albumin, several months
-
-70°C, stable for several months
-
-80°C, 2 weeks, no loss of activity in presence of digitonin
-
-80°C, stable for several weeks
0°C, 2-3 months, change of kinetic properties, larger amount of ATP is required to get the same rate of reaction
-
4°C, enzyme in buffer containing 250 mM imidazole, 1 week, 50% loss of activity
-
4°C, MTK1-39 and mTK2-55 are stable for up to 3 weeks, mTK2 is less stable