2.7.1.21: thymidine kinase
This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.
Word Map on EC 2.7.1.21
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2.7.1.21
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herpes
-
simplex
-
ganciclovir
-
suicide
-
nucleoside
-
hsv-tk
-
acyclovir
-
viruses
-
triphosphate
-
adenovirus
-
prodrugs
-
salvage
-
lymphocyte
-
vaccinia
-
herpesvirus
-
pyrimidine
-
bystander
-
retroviral
-
chloramphenicol
-
positron
-
glioma
-
intratumoral
-
cotransfection
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deoxycytidine
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varicella-zoster
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cytomegalovirus
-
tomography
-
nude
-
lymphoma
-
dna-mediated
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dtmp
-
deoxynucleoside
-
retrovirus-mediated
-
oncolytic
-
adenovirus-mediated
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gene-modified
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deoxyguanosine
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deoxyribonucleoside
-
synthesis
-
medicine
-
herpetic
-
diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
-
zidovudine
-
kinase-deficient
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molecular biology
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3'-azido-3'-deoxythymidine
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pseudorabies
-
replication-competent
-
varicella
-
zoster
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5-fluorocytosine
- 2.7.1.21
- herpes
- simplex
- ganciclovir
-
suicide
- nucleoside
-
hsv-tk
- acyclovir
- viruses
- triphosphate
- adenovirus
-
prodrugs
-
salvage
- lymphocyte
- vaccinia
- herpesvirus
- pyrimidine
-
bystander
-
retroviral
- chloramphenicol
-
positron
- glioma
-
intratumoral
-
cotransfection
- deoxycytidine
-
varicella-zoster
- cytomegalovirus
-
tomography
-
nude
- lymphoma
-
dna-mediated
- dtmp
- deoxynucleoside
-
retrovirus-mediated
-
oncolytic
-
adenovirus-mediated
-
gene-modified
- deoxyguanosine
- deoxyribonucleoside
- synthesis
- medicine
-
herpetic
- diagnostics
-
replication-defective
-
neurovirulence
-
3hthymidine
- zidovudine
-
kinase-deficient
- molecular biology
- 3'-azido-3'-deoxythymidine
- pseudorabies
-
replication-competent
- varicella
- zoster
- 5-fluorocytosine
Reaction
Synonyms
2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK
ECTree
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Turnover Number on EC 2.7.1.21 - thymidine kinase
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TURNOVER NUMBER [1/s] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.0253
(E)-5-(2-bromovinyl)-2'-deoxyuridine
Herpes simplex virus
-
determined by capillary electrophoresis method
0.41
2',3'-dideoxy-2',3'-didehydrothymidine
37°C, pH not specified in the publication
0.33
2',3'-dideoxythymidine
37°C, pH not specified in the publication
0.5
2'-fluoro-5-methyl-beta-L-arabinofuranosyluracil
37°C, pH not specified in the publication
0.47
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.32
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.44
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.35
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.39
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.37
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.45
3-[1(2)-propyltetrazol-5-ylmethyl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.34
3-[1(2)-propyltetrazol-5-yl]thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.238
aciclovir monophosphate
Herpes simplex virus
-
determined by capillary electrophoresis method
0.29
dioxolane thymidine
37°C, pH not specified in the publication
0.0272
ganciclovir monophosphate
Herpes simplex virus
-
determined by capillary electrophoresis method
0.29
2',3'-dideoxy-3'-azidothymidine
37°C, pH not specified in the publication
1.6
2',3'-dideoxy-3'-azidothymidine
82°C, pH not specified in the publication
0.009
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.13
3'-azido-3'-deoxythymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.24
3'-azido-3'-deoxythymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.34
3'-azido-3'-deoxythymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.58
3'-azido-3'-deoxythymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.81
3'-azido-3'-deoxythymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.3
3'-azido-3'-deoxythymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.4
3'-azido-3'-deoxythymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.5
3'-azido-3'-deoxythymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
3.5
3'-azido-3'-deoxythymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
9.3
3'-azido-3'-deoxythymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.74
5-fluoro-2'-deoxyuridine
37°C, pH not specified in the publication
3.1
ATP
-
full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
3.9
ATP
-
thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C
0.17
deoxycytidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.045
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
1.5
deoxyuridine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.02
thymidine
Q8LDP6, Q9FN47
isoform TK1b, pH and temperature not specified in the publication
0.46
thymidine
-
in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C
0.48
thymidine
Q8LDP6, Q9FN47
isoform TK1a, pH and temperature not specified in the publication
0.74
thymidine
-
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
0.97
thymidine
-
mutant enzyme S182T, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.4
thymidine
mutant enzyme M28A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.5
thymidine
mutant enzyme M28I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
1.8
thymidine
mutant enzyme L124A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
2.7
thymidine
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
2.8
thymidine
-
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
4.9
thymidine
-
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
6.4
thymidine
-
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
9.5
thymidine
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication
