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2.7.1.21: thymidine kinase

This is an abbreviated version!
For detailed information about thymidine kinase, go to the full flat file.

Word Map on EC 2.7.1.21

Reaction

ATP
+
thymidine
=
ADP
 +
dTMP

Synonyms

2'-deoxythymidine kinase, ATP-thymidine 5'-phosphotransferase, beta-thymidine kinase-1, beta-thymidine kinase-2, calmodulin-binding protein, CaM-binding protein, CaMBP, CHV-TK, dThd kinase-1, dThd kinase-2, EC 2.7.1.75, EHV4-TK, FHV-TK, HSV-1 TK, HSV1-TK, kinase, deoxythymidine (phosphorylating), kinase, thymidine (phosphorylating), LMJF_21_1210, mitochondrial thymidine kinase, More, Mt-TK, TdR kinase, ThyB, thymidine kinase, thymidine kinase 1, thymidine kinase 2, thymidine kinase 2, mitochondrial, thymidine kinase I, thymidine kinase-1, thymidine kinase-2, thymidylate kinase, TK, TK-1, TK-2, TK1, TK1a, TK1b, TK2, type 1 thymidine kinase, type II thymidine kinase 1, UL23, VVTK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.21 thymidine kinase

Molecular Weight

Molecular Weight on EC 2.7.1.21 - thymidine kinase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
130000
gel filtration
21200
-
x * 21200, calculated from sequence of cDNA
21700
2 * 21700, calculated from amino acid sequence
23300
-
3 * 23300, calculated from amino acid sequence
24000
24800
4 * 24800, calculated from amino acid sequence
24840
-
calculated from amino acid sequence
25000
25400
25494
x * 25494, recombinant enzyme, amino acid sequence calculation
25500
25600
25700
4 * 25700, calculated from amino acid sequence
26000
26100
Q8LDP6, Q9FN47
x * 26100, isoform TK1a, calculated from amino acid sequence
26300
4 * 26300, calculated from amino acid sequence
28000
Tequatrovirus T4
-
x * 28000, SDS-PAGE
29700
4 * 29700, calculated from amino acid sequence
30000
30700
32000
Q9S750; Q9FN47
for subunit alpha pre-incubated without ATP, two different peaks at 52000 and 32000 Da are obtained during gel filtration
340000
-
oligomeric form, gel filtration
36500
Herpes simplex virus type 4
-
x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation
37000
Herpes simplex virus
-
SDS-PAGE
38000
thymidine kinase 1 without ATP, gel filtration
38800
Herpes simplex virus type 4
-
x * 38800, about, recombinant full length enzyme, amino acid sequence calculation, x * 36500, about, recombinant truncated enzyme, amino acid sequence calculation
42000
Herpes simplex virus
-
2 * 42000, SDS-PAGE
42192
-
x * 42192, calculated from amino acid sequence
43000
44000
-
2 * 44000, SDS-PAGE in presence of 2-mercaptoethanol
46000
gel filtration
48000
-
2 * 48000, SDS-PAGE
48800
recombinant enzyme, gel filtration
49000
50000
52000
Q9S750; Q9FN47
for subunit alpha pre-incubated without ATP, two different peaks at 52000 and 32000 Da are obtained during gel filtration
54000
-
gel filtration
55000
gel filtration
57000
58000
gel filtration
59000
60000
64000
for enzyme pre-incubated without ATP, two different peaks at 98000 and 64000 Da are obtained during gel filtration
68440
Murine gammaherpesvirus-68
calculated form amino acid sequence
69000
-
sucrose density gradient centrifugation
70000
70000 - 74000
70000 - 80000
Herpes simplex virus
-
gel filtration
70400 - 80000
-
gel filtration
77100 - 80700
-
native PAGE
80000
80000 - 85000
-
gel filtration
81000
-
sucrose density gradient centrifugation
82000
gel filtration
84000 - 98000
86000
Tequatrovirus T4
-
gel filtration
90000
SEDFIT analysis
92000
-
gel filtration, sucrose density gradient centrifugation
98000
for enzyme pre-incubated without ATP, two different peaks at 98000 and 64000 Da are obtained during gel filtration
additional information