Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania major |
Crystallization (Comment) | Organism |
---|---|
structures of holo forms of the enzyme, in complex with substrate dThd and the non-hydrolysable ATP-analogue P1-(5'-adenosyl)P5-(5'-thymidyl)pentaphosphate | Leishmania major |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dTTP | - |
Leishmania major | |
dUTP | - |
Leishmania major | |
P1-(5'-adenosyl)-P5-(5'-thymidyl)pentaphosphate | - |
Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
2'-deoxythymidine | pH 7.4, 25°C | Leishmania major | |
0.002 | - |
5-bromo-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
0.003 | - |
3'-azido-3'-deoxythymidine | pH 7.4, 25°C | Leishmania major | |
0.0057 | - |
5-fluoro-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
0.011 | - |
2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
0.058 | - |
ATP | K0.5 value, Hill coefficient 1.6, pH 7.4, 25°C | Leishmania major | |
0.0967 | - |
5-hydroxymethyl-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
1.462 | - |
uridine | pH 7.4, 25°C | Leishmania major |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
SEDFIT analysis | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | Q4QC75 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.5 | - |
substrates 2'-deoxythymidine, ATP, pH 7.4, 25°C | Leishmania major |
Storage Stability | Organism |
---|---|
-80°C, stable for several weeks | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxythymidine | - |
Leishmania major | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 2'-deoxyuridine | - |
Leishmania major | ADP + 2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 3'-azido-3'-deoxythymidine | - |
Leishmania major | ADP + 3'-azido-3'-deoxythymidine 5'-phosphate | - |
? | |
ATP + 5-bromo-2'-deoxyuridine | - |
Leishmania major | ADP + 5-bromo-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-fluoro-2'-deoxyuridine | - |
Leishmania major | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + 5-hydroxymethyl-2'-deoxyuridine | - |
Leishmania major | ADP + 5-hydroxymethyl-2'-deoxyuridine 5'-phosphate | - |
? | |
ATP + uridine | - |
Leishmania major | ADP + uridine 5'-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33602, calculated and MALDI-TOF | Leishmania major |
Synonyms | Comment | Organism |
---|---|---|
LMJF_21_1210 | - |
Leishmania major |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
uridine | pH 7.4, 25°C | Leishmania major | |
1.97 | - |
3'-azido-3'-deoxythymidine | pH 7.4, 25°C | Leishmania major | |
2.82 | - |
5-hydroxymethyl-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
3.06 | - |
2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
3.47 | - |
5-bromo-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
4.66 | - |
5-fluoro-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
262 | - |
2'-deoxythymidine | pH 7.4, 25°C | Leishmania major |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Leishmania major |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0011 | - |
pH 7.4, 37°C | Leishmania major | P1-(5'-adenosyl)-P5-(5'-thymidyl)pentaphosphate | |
0.0038 | - |
Hill coefficient 1.6, pH 7.4, 37°C | Leishmania major | dTTP | |
0.187 | - |
pH 7.4, 37°C | Leishmania major | dUTP |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
uridine | pH 7.4, 25°C | Leishmania major | |
29.1 | - |
5-hydroxymethyl-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
276 | - |
2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
651 | - |
3'-azido-3'-deoxythymidine | pH 7.4, 25°C | Leishmania major | |
814 | - |
5-fluoro-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
1690 | - |
5-bromo-2'-deoxyuridine | pH 7.4, 25°C | Leishmania major | |
2380 | - |
2'-deoxythymidine | pH 7.4, 25°C | Leishmania major |