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Literature summary for 2.7.1.21 extracted from

  • Ranjbarian, F.; Vodnala, M.; Vodnala, S.M.; Rofougaran, R.; Thelander, L.; Hofer, A.
    Trypanosoma brucei thymidine kinase is tandem protein consisting of two homologous parts, which together enable efficient substrate binding (2012), J. Biol. Chem., 287, 17628-17636.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol the enzyme is stimulated by dithiothreitol up to a concentration of 2 mM Trypanosoma brucei
KCl weak stimulation Trypanosoma brucei
NaCl weak stimulation Trypanosoma brucei
potassium acetate weak stimulation Trypanosoma brucei

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) cells Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
sulfate competitive inhibitor of ATP binding Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
thymidine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.03
-
5-fluorodeoxyuridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.046
-
thymidine thymidine kinase domain 2, at pH 7.8 and 37°C Trypanosoma brucei
0.08
-
deoxyuridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.098
-
ATP thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
0.18
-
ATP full-length enzyme, with 0.0005 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
0.21
-
ATP full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
0.22
-
ATP full-length enzyme, with 0.005 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
0.32
-
ATP full-length enzyme, with 0.5 mM deoxyinosine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
0.43
-
deoxyuridine thymidine kinase domain 2, at pH 7.8 and 37°C Trypanosoma brucei
2.5
-
deoxyinosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
2.6
-
deoxyguanosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
7.8
-
uridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
8.4
-
deoxyadenosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-
Trypanosoma brucei TC221
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-fluorodeoxyuridine
-
Trypanosoma brucei ADP + 5-fluorodeoxyuridine 5'-phosphate
-
?
ATP + deoxyadenosine
-
Trypanosoma brucei ADP + dAMP
-
?
ATP + deoxyadenosine
-
Trypanosoma brucei TC221 ADP + dAMP
-
?
ATP + deoxycytidine
-
Trypanosoma brucei ADP + dCMP
-
?
ATP + deoxycytidine
-
Trypanosoma brucei TC221 ADP + dCMP
-
?
ATP + deoxyguanosine about 20% activity compared to thymidine Trypanosoma brucei ADP + dGMP
-
?
ATP + deoxyguanosine about 20% activity compared to thymidine Trypanosoma brucei TC221 ADP + dGMP
-
?
ATP + deoxyinosine about 30% activity compared to thymidine Trypanosoma brucei ADP + dIMP
-
?
ATP + deoxyinosine about 30% activity compared to thymidine Trypanosoma brucei TC221 ADP + dIMP
-
?
ATP + deoxyuridine about 185% activity compared to thymidine Trypanosoma brucei ADP + dUMP
-
?
ATP + thymidine best substrate combination, 100% activity Trypanosoma brucei ADP + dTMP
-
?
ATP + uridine
-
Trypanosoma brucei ADP + UMP
-
?
CTP + thymidine
-
Trypanosoma brucei CDP + dTMP
-
?
GTP + thymidine
-
Trypanosoma brucei GDP + dTMP
-
?
additional information domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine Trypanosoma brucei ?
-
?
additional information domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine Trypanosoma brucei TC221 ?
-
?
UTP + thymidine
-
Trypanosoma brucei UDP + dTMP
-
?

Subunits

Subunits Comment Organism
monomer
-
Trypanosoma brucei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.047
-
deoxyadenosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.34
-
deoxyguanosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.8
-
deoxyinosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
1.5
-
uridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
3.1
-
thymidine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
3.1
-
ATP full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
3.5
-
thymidine thymidine kinase domain 2, at pH 7.8 and 37°C Trypanosoma brucei
3.6
-
5-fluorodeoxyuridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
3.9
-
ATP thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
5.1
-
deoxyuridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
5.3
-
deoxyuridine thymidine kinase domain 2, at pH 7.8 and 37°C Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Trypanosoma brucei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0056
-
deoxyadenosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.13
-
deoxyguanosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.19
-
uridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
0.32
-
deoxyinosine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
12
-
deoxyuridine thymidine kinase domain 2, at pH 7.8 and 37°C Trypanosoma brucei
15
-
ATP full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
40
-
ATP thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C Trypanosoma brucei
63
-
deoxyuridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
76
-
thymidine thymidine kinase domain 2, at pH 7.8 and 37°C Trypanosoma brucei
120
-
5-fluorodeoxyuridine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei
400
-
thymidine full-length enzyme, at pH 7.8 and 37°C Trypanosoma brucei