Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | the enzyme is stimulated by dithiothreitol up to a concentration of 2 mM | Trypanosoma brucei | |
KCl | weak stimulation | Trypanosoma brucei | |
NaCl | weak stimulation | Trypanosoma brucei | |
potassium acetate | weak stimulation | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3) cells | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfate | competitive inhibitor of ATP binding | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
thymidine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.03 | - |
5-fluorodeoxyuridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.046 | - |
thymidine | thymidine kinase domain 2, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.08 | - |
deoxyuridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.098 | - |
ATP | thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.18 | - |
ATP | full-length enzyme, with 0.0005 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.21 | - |
ATP | full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.22 | - |
ATP | full-length enzyme, with 0.005 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.32 | - |
ATP | full-length enzyme, with 0.5 mM deoxyinosine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.43 | - |
deoxyuridine | thymidine kinase domain 2, at pH 7.8 and 37°C | Trypanosoma brucei | |
2.5 | - |
deoxyinosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
2.6 | - |
deoxyguanosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
7.8 | - |
uridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
8.4 | - |
deoxyadenosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Trypanosoma brucei TC221 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-fluorodeoxyuridine | - |
Trypanosoma brucei | ADP + 5-fluorodeoxyuridine 5'-phosphate | - |
? | |
ATP + deoxyadenosine | - |
Trypanosoma brucei | ADP + dAMP | - |
? | |
ATP + deoxyadenosine | - |
Trypanosoma brucei TC221 | ADP + dAMP | - |
? | |
ATP + deoxycytidine | - |
Trypanosoma brucei | ADP + dCMP | - |
? | |
ATP + deoxycytidine | - |
Trypanosoma brucei TC221 | ADP + dCMP | - |
? | |
ATP + deoxyguanosine | about 20% activity compared to thymidine | Trypanosoma brucei | ADP + dGMP | - |
? | |
ATP + deoxyguanosine | about 20% activity compared to thymidine | Trypanosoma brucei TC221 | ADP + dGMP | - |
? | |
ATP + deoxyinosine | about 30% activity compared to thymidine | Trypanosoma brucei | ADP + dIMP | - |
? | |
ATP + deoxyinosine | about 30% activity compared to thymidine | Trypanosoma brucei TC221 | ADP + dIMP | - |
? | |
ATP + deoxyuridine | about 185% activity compared to thymidine | Trypanosoma brucei | ADP + dUMP | - |
? | |
ATP + thymidine | best substrate combination, 100% activity | Trypanosoma brucei | ADP + dTMP | - |
? | |
ATP + uridine | - |
Trypanosoma brucei | ADP + UMP | - |
? | |
CTP + thymidine | - |
Trypanosoma brucei | CDP + dTMP | - |
? | |
GTP + thymidine | - |
Trypanosoma brucei | GDP + dTMP | - |
? | |
additional information | domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine | Trypanosoma brucei | ? | - |
? | |
additional information | domain 1 of thymidine kinase is catalytically inactive but improves substrate binding by domain 2. The enzyme shows the following order of catalytic efficiencies: thymidine > deoxyuridine >> deoxyinosine > deoxyguanosine. The enzyme shows no activity with deoxyadenosine and deoxycytidine | Trypanosoma brucei TC221 | ? | - |
? | |
UTP + thymidine | - |
Trypanosoma brucei | UDP + dTMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
deoxyadenosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.34 | - |
deoxyguanosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.8 | - |
deoxyinosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
1.5 | - |
uridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
3.1 | - |
thymidine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
3.1 | - |
ATP | full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
3.5 | - |
thymidine | thymidine kinase domain 2, at pH 7.8 and 37°C | Trypanosoma brucei | |
3.6 | - |
5-fluorodeoxyuridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
3.9 | - |
ATP | thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
5.1 | - |
deoxyuridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
5.3 | - |
deoxyuridine | thymidine kinase domain 2, at pH 7.8 and 37°C | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
deoxyadenosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.13 | - |
deoxyguanosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.19 | - |
uridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
0.32 | - |
deoxyinosine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
12 | - |
deoxyuridine | thymidine kinase domain 2, at pH 7.8 and 37°C | Trypanosoma brucei | |
15 | - |
ATP | full-length enzyme, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
40 | - |
ATP | thymidine kinase domain 2, with 0.05 mM thymidine as cosubstrate, at pH 7.8 and 37°C | Trypanosoma brucei | |
63 | - |
deoxyuridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
76 | - |
thymidine | thymidine kinase domain 2, at pH 7.8 and 37°C | Trypanosoma brucei | |
120 | - |
5-fluorodeoxyuridine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei | |
400 | - |
thymidine | full-length enzyme, at pH 7.8 and 37°C | Trypanosoma brucei |