Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0049 | - |
thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0174 | - |
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0318 | - |
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0356 | - |
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0373 | - |
3-[1(2)-propyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0552 | - |
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0552 | - |
3-[1(2)-propyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0563 | - |
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.0799 | - |
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-(benzylcarbamimidoyl)thymidine | 14.9% activity compared to thymidine | Homo sapiens | ADP + 3-(benzylcarbamimidoyl)thymidine 5'-phosphate | - |
? | |
ATP + 3-(butylcarbamimidoyl)thymidine | 1.2% activity compared to thymidine | Homo sapiens | ADP + 3-(butylcarbamimidoyl)thymidine 5'-phosphate | - |
? | |
ATP + 3-(phenylcarbamimidoyl)thymidine | 1.9% activity compared to thymidine | Homo sapiens | ADP + 3-(phenylcarbamimidoyl)thymidine 5'-phosphate | - |
? | |
ATP + 3-(tetrazol-5-ylmethyl)thymidine | 39.5% activity compared to thymidine | Homo sapiens | ADP + 3-(tetrazol-5-ylmethyl)thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine | 63.7% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine | 42.5% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine | 52.4% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine | 55.6% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine | 58.2% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine | 42.9% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-propyltetrazol-5-ylmethyl]thymidine | 55.7% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-propyltetrazol-5-ylmethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[1(2)-propyltetrazol-5-yl]thymidine | 57.6% activity compared to thymidine | Homo sapiens | ADP + 3-[1(2)-propyltetrazol-5-yl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[2-(closo-1,7-carboranyl)ethylcarbamimidoyl]thymidine | 16.3% activity compared to thymidine | Homo sapiens | ADP + 3-[2-(closo-1,7-carboranyl)ethylcarbamimidoyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[2-imino-2-(benzylamino)ethyl]thymidine | 16.8% activity compared to thymidine | Homo sapiens | ADP + 3-[2-imino-2-(benzylamino)ethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[2-imino-2-(butylamino)ethyl]thymidine | 2.3% activity compared to thymidine | Homo sapiens | ADP + 3-[2-imino-2-(butylamino)ethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[2-imino-2-(phenylamino)ethyl]thymidine | 11.3% activity compared to thymidine | Homo sapiens | ADP + 3-[2-imino-2-(phenylamino)ethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[2-imino-2-(propylamino)ethyl]thymidine | 1.9% activity compared to thymidine | Homo sapiens | ADP + 3-[2-imino-2-(propylamino)ethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)ethylamino]ethyl]thymidine | 3.0% activity compared to thymidine | Homo sapiens | ADP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)ethylamino]ethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)propylamino]ethyl]thymidine | 5.6% activity compared to thymidine | Homo sapiens | ADP + 3-[2-imino-2-[2-(closo-1,7-carboranyl)propylamino]ethyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[3-(closo-1,7-carboranyl)propylcarbamimidoyl]thymidine | 15.5% activity compared to thymidine | Homo sapiens | ADP + 3-[3-(closo-1,7-carboranyl)propylcarbamimidoyl]thymidine 5'-phosphate | - |
? | |
ATP + 3-[4-(closo-1,7-carboranyl)butylcarbamimidoyl]thymidine | 27.3% activity compared to thymidine | Homo sapiens | ADP + 3-[4-(closo-1,7-carboranyl)butylcarbamimidoyl]thymidine 5'-phosphate | - |
? | |
ATP + thymidine | 100% activity | Homo sapiens | ADP + dTMP | - |
? | |
additional information | no activity with 3-(propylcarbamimidoyl)thymidine and 3-(tetrazol-5-yl)thymidine | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
thymidine kinase 1 | - |
Homo sapiens |
TK1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.34 | - |
3-[1(2)-propyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.35 | - |
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.37 | - |
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-yl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.39 | - |
3-[1(2)-closo-1,7-carboranylpropyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.44 | - |
3-[1(2)-closo-1,7-carboranylethyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.45 | - |
3-[1(2)-propyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.46 | - |
thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens | |
0.47 | - |
3-[1(2)-closo-1,7-carboranylbutyltetrazol-5-ylmethyl]thymidine | in 25 mM Tris-HCl (pH 7.6), 5 mM MgCl2, 125 mM KCl, 10 mM dithiothreitol, and 0.5 mg/ml bovine serum albumin, at 37°C | Homo sapiens |