Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.5.1.6: methionine adenosyltransferase

This is an abbreviated version!
For detailed information about methionine adenosyltransferase, go to the full flat file.

Word Map on EC 2.5.1.6

Reaction

ATP
+
L-methionine
 +
H2O
=
phosphate
 +
diphosphate
 +
S-adenosyl-L-methionine

Synonyms

(Met) adenosyltransferase 4, adenosylmethionine synthase, adenosylmethionine synthetase, AdoMet synthase, AdoMet synthease, AdoMet synthetase, AdoMetS, ATP-methionine adenosyltransferase, ATP: L-methionine S-adenosyltransferase, EC 2.4.2.13, MAT, MAT I, MAT II, MAT IIalpha, MAT III, MAT1A, MAT2, MAT2A, MAT2beta, MAT3, MAT4, MATalpha1, MATalpha2, methionine adenosyl transferase 2A, methionine adenosyltransferase, methionine adenosyltransferase 2A, methionine adenosyltransferase 2beta, methionine adenosyltransferase 4, methionine adenosyltransferase alpha1, methionine adenosyltransferase II, methionine adenosyltransferase II-alpha, methionine adenosyltransferase IIalpha, methionine S-adenosyltransferase, methionine-activating enzyme, MetK, Mj-MAT, MJ1208, pDS16, PF1866, PfMAT, S-adenosyl-L-methionine synthetase, S-adenosyl-Lmethionine synthetase, S-adenosyl-Met synthetase 3, S-adenosylmethionine synthase, S-adenosylmethionine synthetase, S-adenosylmethionine synthetase 1, S-adenosylmethionine synthetase 2, S-adenosylmethionine synthetase 3, S-adenosylmethionine synthetase A, S-adenosylmethionine synthetase B, S-adenosylmethionine-L-synthetase, SAM synthase, SAM synthetase, SAM-s, Sam1, SAM2, SAMS, SAMS1, SAMS2, SMAT, SSO0199

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.6 methionine adenosyltransferase

Cloned

Cloned on EC 2.5.1.6 - methionine adenosyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a multiple-copy integrative plasmid pYMIKP-SAM2 is introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001
alpha subunit expressed in Escherichia coli NM522 strain
-
chromosomal integration and expression in Saccharopolyspora erythraea strain E2 resulting in the overexpressing strain E1, subcloning in Escherichia coli strains ET12567 and DH5alpha
-
cloning and sequencing of the MAT 2A cDNA from lung epithelial cells reveals one silent nucleotide substitution compared to that expressed in Jurkat cells
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21(DE3)
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)codon plus/pMJ1208-strain with a decahistidine tag on the N-terminus
-
expressed in Escherichia coli M15(Qiagen)
expressed in Escherichia coli strain BL21(DE3) RIL
expressed in Escherichia coli strain SG10039
-
expressed in Escherichia coli XL-1Blue strain
expressed in Hep-G2 cells, HuH-7 cells, and HCC cells
-
expressed in M15 bacteria
-
expressed in Nicotiana tabacum
expressed in Pichia pastoris
expressed in Pichia pastoris G115S cells
-
expressed in Pichia pastoris strain GS115
expressed in Streptomyces actuosus
expression in Escherichia coli
expression in Escherichia coli DH5alpha
-
expression in Escheriochia coli
expression in Nicotiana tabacum
-
expression in the Escherichia coli strain M15
gene MAT, DNA and amino acid sequence determination and analysis, expression analysis
gene MAT-2, stable expression in Leishmania donovani promastigotes, phenotypic effects, overview
-
gene metK, DNA and amino acid sequence determination and analysis, expression in and complementation of an Escherichia coli metK deletion strain
gene metK, expression in a metK deletion mutant Escherichia coli strain MOB1490
-
gene metK, expression in Bacillus subtilis
-
gene SAM-s, DNA and amino acid sequence determination and analysis, a putative purine-rich ribosomal binding site is located 3 bp upstream of the GTG start codon, potential -35 and -10 consensus promoter sequences, expression of His-tagged enzyme in Escherichia coli strain XL-1 Blue, overexpression in Streptomyces avermitilis leads to 2fold increased synthesis of the antibiotic avermectin, overexpression in Streptomyces peucetius leads to 3.5fold increased synthesis of the antibiotic doxorubicin
gene SAMS1, a housekeeping gene, DNA and amino acid sequence determination and analysis, expression analysis and regulation, overview, expression of soluble His-tagged isozyme SAMS1 in Escherichia coli strain M15
gene SAMS2, a housekeeping gene, DNA and amino acid sequence determination and analysis, expression analysis and regulation, overview
gene SAMS3, a housekeeping gene, DNA and amino acid sequence determination and analysis, expression analysis and regulation, overview
gene SAMS4, a housekeeping gene, DNA and amino acid sequence determination and analysis, expression analysis and regulation, overview
His-tagged recombinant MAT II alpha2 subunit expressed in COS-1 cells
-
His6-tagged fusion protein expressed in Escherichia coli
-
subunit MAT2beta
the gene is transformed into Nicotiana benthamiana Domin to investigate its function under low temperature stress. The levels of MsSAMS transcripts increase over a 48 h period in response to cold treatment and increase cold tolerance in the transgenic plants. The change in cold tolerance is confirmed by determining the recovery rate of the plants and by measurement of ion leakage after cold treatment. Based on this morphological analysis, transgenic plants exhibit an increase in cell wall thickness compared to control plants
-
three isoenzymes expressed in Escherichia coli