Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Thermus thermophilus | |
Fe2+ | - |
Thermus thermophilus | |
Mn | - |
Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
L-methionine | at pH 8.0 and 70°C | Thermus thermophilus | |
4.19 | - |
ATP | at pH 8.0 and 70°C | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | high activity in the presence of 20 mM Zn2+ | Thermus thermophilus | |
Zn2+ | highest activity in the presence of 20 mM Zn2+ | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Thermus thermophilus | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q72I53 | - |
- |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | Q72I53 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Thermus thermophilus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | - |
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
MAT | - |
Thermus thermophilus |
methionine adenosyltransferase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | the enzyme shows activity at a broad temperature range, from 30 to 90°C. The reaction rate increases significantly with temperature, and reaches maximum at 80°C | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | the enzyme retains 60% of its activity after incubation at 70°C for 24 h. The enzyme is nearly inactivated after 1 h and 10 h incubation at 90°C and 80°C, respectively | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thermus thermophilus |