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Literature summary for 2.5.1.6 extracted from

  • Wijayasinghe, Y.S.; Blumenthal, R.M.; Viola, R.E.
    Producing proficient methyl donors from alternative substrates of S-adenosylmethionine synthetase (2014), Biochemistry, 53, 1521-1526.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Neisseria meningitidis
expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Neisseria meningitidis
Mg2+ required Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Neisseria meningitidis
-
phosphate + diphosphate + S-adenosyl-L-methionine
-
?
ATP + L-methionine + H2O Campylobacter jejuni
-
phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-
Neisseria meningitidis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Neisseria meningitidis
nickel affinity column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Neisseria meningitidis phosphate + diphosphate + S-adenosyl-L-methionine
-
?
ATP + L-methionine + H2O
-
Campylobacter jejuni phosphate + diphosphate + S-adenosyl-L-methionine
-
?
ATP + L-methionine ethyl ester + H2O excellent substrate Neisseria meningitidis ?
-
?
ATP + L-methionine ethyl ester + H2O excellent substrate Campylobacter jejuni ?
-
?
ATP + L-methionine methyl ester + H2O
-
Neisseria meningitidis ?
-
?
ATP + L-methionine methyl ester + H2O
-
Campylobacter jejuni ?
-
?
additional information N-acetyl-L-methionine and N,N-dimethyl-L-methionine are very poor substrates Neisseria meningitidis ?
-
?
additional information N-acetyl-L-methionine and N,N-dimethyl-L-methionine are very poor substrates Campylobacter jejuni ?
-
?

Synonyms

Synonyms Comment Organism
AdoMet synthetase
-
Neisseria meningitidis
AdoMet synthetase
-
Campylobacter jejuni
MetK
-
Neisseria meningitidis
MetK
-
Campylobacter jejuni
S-adenosylmethionine synthetase
-
Neisseria meningitidis
S-adenosylmethionine synthetase
-
Campylobacter jejuni