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2.5.1.6: methionine adenosyltransferase

This is an abbreviated version!
For detailed information about methionine adenosyltransferase, go to the full flat file.

Word Map on EC 2.5.1.6

Reaction

ATP
+
L-methionine
+
H2O
=
phosphate
+
diphosphate
+
S-adenosyl-L-methionine

Synonyms

(Met) adenosyltransferase 4, adenosylmethionine synthase, adenosylmethionine synthetase, AdoMet synthase, AdoMet synthease, AdoMet synthetase, AdoMetS, ATP-methionine adenosyltransferase, ATP: L-methionine S-adenosyltransferase, EC 2.4.2.13, MAT, MAT I, MAT II, MAT IIalpha, MAT III, MAT1A, MAT2, MAT2A, MAT2beta, MAT3, MAT4, MATalpha1, MATalpha2, methionine adenosyl transferase 2A, methionine adenosyltransferase, methionine adenosyltransferase 2A, methionine adenosyltransferase 2beta, methionine adenosyltransferase 4, methionine adenosyltransferase alpha1, methionine adenosyltransferase II, methionine adenosyltransferase II-alpha, methionine adenosyltransferase IIalpha, methionine S-adenosyltransferase, methionine-activating enzyme, MetK, Mj-MAT, MJ1208, pDS16, PF1866, PfMAT, S-adenosyl-L-methionine synthetase, S-adenosyl-Lmethionine synthetase, S-adenosyl-Met synthetase 3, S-adenosylmethionine synthase, S-adenosylmethionine synthetase, S-adenosylmethionine synthetase 1, S-adenosylmethionine synthetase 2, S-adenosylmethionine synthetase 3, S-adenosylmethionine synthetase A, S-adenosylmethionine synthetase B, S-adenosylmethionine-L-synthetase, SAM synthase, SAM synthetase, SAM-s, Sam1, SAM2, SAMS, SAMS1, SAMS2, SMAT, SSO0199

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.6 methionine adenosyltransferase

Crystallization

Crystallization on EC 2.5.1.6 - methionine adenosyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using 50 mM Tris-HCl pH 8.0, 200 mM KCl, 2% (w/v) ethylene glycol, 1 mM dithiothreitol, 12-16% (w/v) polyethylene glycol 8000
-
hanging-drop vapor diffusion method at 20°C
hexagonal bipyramid crystals of the pyrophosphate complex grown from a solution containing phosphate, diphosphate, sulfate and Mg2+. Crystals of the ADP complex grown obtained from a solution containing phosphate, ADP, sulfate and Mg2+. Crystals of the BrADP complex obtained by a soaking method: pyrophosphate-MAT crystals obtained from a solution containing phosphate, sulfate and Mg2+, and replaced the mother liquor with a solution containing BrATP, Tris-HCl buffer, Mg+2 and ammonium sulfate
in complex with 5’-adenylyl imido triphosphate and methionine
method of vapor diffusion, hexagonal bipiramid crystals in the presence of Mg2+ and diphosphate
-
quantum mechanical/molecular mechanical calculations, exploiting structures of the active crystalline enzyme, based on PDB files 1P7L and 1RG9
in complex with S-adenosyl-L-methionine, L-methionine, and 4-nitrophenyl phosphate, vapor diffusion method, using 0.1 M HEPES pH 7.5 and 30% (w/v) PEG 600
sitting drop vapor diffusion method
sitting drop vapor diffusion method, using
construction of a 3D-homology model. This model predicts preservation of the chain topology and three-domain organization typical of this family, locates the least stable structural elements at the flat contact surface between monomers, and shows that alterations in all three domains are required for dimer dissociation
hanging drop vapour diffusion method, structural analysis of the Pyrococcus furiosus methionine adenosyltransferase captured in the unliganded, substrate-and product-bound states
in complex with inhibitor (2S,4S)-amino-4,5-epoxypentanoic acid
sitting drop method at 20°C, crystallization of the enzyme in the S-adenosylmethionine bound, S-adenosylethionine bound and unbound forms
sitting drop vapor diffusion method, using 22% (w/v) polyethylene glycol 3350 and 0.2 M sodium citrate
-
hanging drop vapor diffusion method, using 0.01 M iron-(III)-chloride hexahydrate, 0.1 M sodium citrate tribasic dehydrate pH 5.6 and 10% (v/v) Jeffamine M-600