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Literature summary for 2.5.1.6 extracted from

  • Wang, F.; Singh, S.; Zhang, J.; Huber, T.D.; Helmich, K.E.; Sunkara, M.; Hurley, K.A.; Goff, R.D.; Bingman, C.A.; Morris, A.J.; Thorson, J.S.; Phillips, G.N.
    Understanding molecular recognition of promiscuity of thermophilic methionine adenosyltransferase sMAT from Sulfolobus solfataricus (2014), FEBS J., 281, 224-239.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop method at 20°C, crystallization of the enzyme in the S-adenosylmethionine bound, S-adenosylethionine bound and unbound forms Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 L-methionine pH 8.0, 37°C Saccharolobus solfataricus
0.0028
-
 L-methionine pH 8.0, 65°C Saccharolobus solfataricus
0.0056
-
 L-ethionine pH 8.0, 37°C Saccharolobus solfataricus
0.007
-
 L-ethionine pH 8.0, 65°C Saccharolobus solfataricus
0.0145
-
 ATP pH 8.0, 65°C, cosubstrate: L-methionine Saccharolobus solfataricus
0.0592
-
 ATP pH 8.0, 65°C, cosubstrate: L-ethionine Saccharolobus solfataricus
0.0686
-
 ATP pH 8.0, 37°C, cosubstrate: L-methionine Saccharolobus solfataricus
0.0969
-
 ATP pH 8.0, 37°C, cosubstrate: L-ethionine Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q980S9
-
-
Saccharolobus solfataricus P2 Q980S9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O 10% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid + H2O 10% of the turnover rate compared to L-methionine Saccharolobus solfataricus P2 phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O 30% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid + H2O 30% of the turnover rate compared to L-methionine Saccharolobus solfataricus P2 phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(but-3-yn-1-ylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O 70% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(butylselanyl)butanoic acid + H2O 70% of the turnover rate compared to L-methionine Saccharolobus solfataricus P2 phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(butylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(butylsulfanyl)butanoic acid + H2O 27% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(butylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(ethylselanyl)butanoic acid + H2O 76% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(ethylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(ethylsulfanyl)butanoic acid + H2O 84% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(ethylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid + H2O 30% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid + H2O 27% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-en-1-ylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid + H2O 28% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid + H2O 36% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(prop-2-yn-1-ylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid + H2O 40% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propan-2-ylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid + H2O 53% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propan-2-ylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(propylselanyl)butanoic acid + H2O 66% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-(propylselanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-(propylsulfanyl)butanoic acid + H2O 44% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-(propylsulfanyl)butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid + H2O 50% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-azido ethyl)sulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(2-methyl propyl)sulfanyl] butanoic acid + H2O 57% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methyl propyl)sulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid + H2O 46% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)selanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid + H2O 15% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2-methylprop-2-en-1-yl)sulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid + H2O 27% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-but-2-en-1-ylsulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid + H2O 25% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(2E)-penta-2,4-dien-1-ylsulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid + H2O 60% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-azido propyl)sulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(3-methyl butyl)sulfanyl]butanoic acid + H2O 36% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methyl butyl)sulfanyl] butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid + H2O 44% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)selanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid + H2O 12% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(3-methylbut-2-en-1-yl)sulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(cyanomethyl) sulfanyl]butanoic acid + H2O 68% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[(cyanomethyl)sulfanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid + H2O 12% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[(cyanomethyl)selanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid + H2O 38% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-aminobut-2-en-1-yl]selanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid + H2O 24% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]selanyl]butanoic acid
-
 ?
ATP + (2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid + H2O 28% of the turnover rate compared to L-methionine Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-(2S)-2-amino-4-[[(2E)-4-azidobut-2-en-1-yl]sulfanyl]butanoic acid
-
 ?
ATP + L-ethionine + H2O
-
 Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-L-ethionine
-
 ?
ATP + L-methionine + H2O
-
 Saccharolobus solfataricus phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-methionine + H2O
-
 Saccharolobus solfataricus P2 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?
ATP + L-selenomethionine + H2O as active as L-methionine Saccharolobus solfataricus phosphate + diphosphate + Se-adenosyl-L-selenomethionine
-
 ?
additional information the enzyme has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP Saccharolobus solfataricus ?
-
 ?
additional information the enzyme has the ability to produce a range of differentially alkylated AdoMet analogs in the presence of non-native methionine analogs and ATP Saccharolobus solfataricus P2 ?
-
 ?

Synonyms

Synonyms Comment Organism
SSO0199 locus name Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0328
-
 ATP pH 8.0, 37°C, cosubstrate: L-methionine Saccharolobus solfataricus
0.0343
-
 L-methionine pH 8.0, 37°C Saccharolobus solfataricus
0.039
-
 L-ethionine pH 8.0, 37°C Saccharolobus solfataricus
0.041
-
 ATP pH 8.0, 37°C, cosubstrate: L-ethionine Saccharolobus solfataricus
0.047
-
 ATP pH 8.0, 65°C, cosubstrate: L-methionine Saccharolobus solfataricus
0.049
-
 L-methionine pH 8.0, 65°C Saccharolobus solfataricus
0.05
-
 L-ethionine pH 8.0, 65°C Saccharolobus solfataricus
0.052
-
 ATP pH 8.0, 65°C, cosubstrate: L-ethionine Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.43
-
 ATP pH 8.0, 37°C, cosubstrate: L-ethionine Saccharolobus solfataricus
0.48
-
 ATP pH 8.0, 37°C, cosubstrate: L-methionine Saccharolobus solfataricus
0.87
-
 ATP pH 8.0, 65°C, cosubstrate: L-ethionine Saccharolobus solfataricus
3.23
-
 ATP pH 8.0, 65°C, cosubstrate: L-methionine Saccharolobus solfataricus
6.95
-
 L-ethionine pH 8.0, 37°C Saccharolobus solfataricus
7.12
-
 L-ethionine pH 8.0, 65°C Saccharolobus solfataricus
14.92
-
 L-methionine pH 8.0, 37°C Saccharolobus solfataricus
17.37
-
 L-methionine pH 8.0, 65°C Saccharolobus solfataricus