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2.5.1.6: methionine adenosyltransferase

This is an abbreviated version!
For detailed information about methionine adenosyltransferase, go to the full flat file.

Word Map on EC 2.5.1.6

Reaction

ATP
+
L-methionine
 +
H2O
=
phosphate
 +
diphosphate
 +
S-adenosyl-L-methionine

Synonyms

(Met) adenosyltransferase 4, adenosylmethionine synthase, adenosylmethionine synthetase, AdoMet synthase, AdoMet synthease, AdoMet synthetase, AdoMetS, ATP-methionine adenosyltransferase, ATP: L-methionine S-adenosyltransferase, EC 2.4.2.13, MAT, MAT I, MAT II, MAT IIalpha, MAT III, MAT1A, MAT2, MAT2A, MAT2beta, MAT3, MAT4, MATalpha1, MATalpha2, methionine adenosyl transferase 2A, methionine adenosyltransferase, methionine adenosyltransferase 2A, methionine adenosyltransferase 2beta, methionine adenosyltransferase 4, methionine adenosyltransferase alpha1, methionine adenosyltransferase II, methionine adenosyltransferase II-alpha, methionine adenosyltransferase IIalpha, methionine S-adenosyltransferase, methionine-activating enzyme, MetK, Mj-MAT, MJ1208, pDS16, PF1866, PfMAT, S-adenosyl-L-methionine synthetase, S-adenosyl-Lmethionine synthetase, S-adenosyl-Met synthetase 3, S-adenosylmethionine synthase, S-adenosylmethionine synthetase, S-adenosylmethionine synthetase 1, S-adenosylmethionine synthetase 2, S-adenosylmethionine synthetase 3, S-adenosylmethionine synthetase A, S-adenosylmethionine synthetase B, S-adenosylmethionine-L-synthetase, SAM synthase, SAM synthetase, SAM-s, Sam1, SAM2, SAMS, SAMS1, SAMS2, SMAT, SSO0199

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.6 methionine adenosyltransferase

Temperature Stability

Temperature Stability on EC 2.5.1.6 - methionine adenosyltransferase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 40
-
the enzyme activity remains stable after 2 h preincubation at 20-40°C in Tris-HCl (pH 8.9)
22
-
stable for several hours, if protein concentration is above 0.1 mg/ml and ionic strength is at least 50 mM
30 - 40
enzyme activity is significantly reduced at temperatures below 30°C or above 40°C
35
-
the enzyme is stable with a residual activity over 85% after incubation at 35°C for 2 h
37
-
30% loss of activity after 12 h, complete loss of activity after 36-48 h, soluble enzyme. 63% of the initial enzyme activity of immobilized MAT was remained after 168 h incubation. Comparing the t1/2 values at 37 °C, the immobilized enzyme is almost 25fold more stable than the free enzyme
40 - 60
the enzyme activity is unchanged between 40 and 60°C after 24 h, while at 80°C, the enzyme activity drops to zero in 15 min
45
-
stable up to
47 - 51
-
Tm-value, irreversible thermal denaturation
70 - 90
the enzyme retains 60% of its activity after incubation at 70°C for 24 h. The enzyme is nearly inactivated after 1 h and 10 h incubation at 90°C and 80°C, respectively
80
1 h, 20% loss of activity
95
half-life: 23 min
additional information
-
preincubation with ATP protects against thermal inactivation