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Literature summary for 2.5.1.6 extracted from
- Biochemical characterization of a thermostable adenosylmethionine synthetase from the archaeon Pyrococcus furiosus with high catalytic power (2015), Appl. Biochem. Biotechnol., 175, 2916-33.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escheriochia coli | Pyrococcus furiosus |
General Stability
| General Stability | Organism |
|---|---|
| limited proteolysis experiments indicate that the proteolytic cleavage site is localized at Lys148 and that the C-terminal peptide is necessary for the integrity of the active site | Pyrococcus furiosus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.031 | - |
L-methionine | pH 8.0, 80°C | Pyrococcus furiosus |  |
| 0.115 | - |
ATP | pH 8.0, 80°C | Pyrococcus furiosus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolutely dependent upon the presence of Mg2+, suggesting that a Mg2+-ATP complex functions as substrate. Maximal activity is obtained at a Mg2+ concentration of 20 mM | Pyrococcus furiosus | |
| additional information | no stimulatory effect by K+ has been observed even at high concentration (40 mM) | Pyrococcus furiosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44279 | - |
2 * 44279, calculated from sequence | Pyrococcus furiosus |
| 45000 | - |
2 * 45000, SDS-PAGE | Pyrococcus furiosus |
| 90000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | Pyrococcus furiosus | the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | midpoint transition is 3.2 M | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | Q8TZW1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
pH 8.0, 80°C | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + H2O | - |
Pyrococcus furiosus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
| ATP + L-methionine + H2O | the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine | Pyrococcus furiosus | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 45000, SDS-PAGE | Pyrococcus furiosus |
| homodimer | 2 * 44279, calculated from sequence | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PF1866 | - |
Pyrococcus furiosus |
| PfMAT | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 105 | 70°C: about 55% of maximal activity, 105°C: about 60% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
1 h, stable | Pyrococcus furiosus |
| 80 | - |
1 h, 20% loss of activity | Pyrococcus furiosus |
| 95 | - |
half-life: 23 min | Pyrococcus furiosus |
| 99 | - |
Tm-value | Pyrococcus furiosus |
| 100 | - |
half-life: 5 min | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.5 | - |
ATP | pH 8.0, 80°C | Pyrococcus furiosus | |
| 7.5 | - |
L-methionine | pH 8.0, 80°C | Pyrococcus furiosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Pyrococcus furiosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | half of the maximal activity at pH 7 and 9 | Pyrococcus furiosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pyrococcus furiosus | calculated from sequence | - |
5.52 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme plays a key role in the biogenesis of S-adenosyl-L-methionine | Pyrococcus furiosus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 65.2 | - |
ATP | pH 8.0, 80°C | Pyrococcus furiosus | |
| 242 | - |
L-methionine | pH 8.0, 80°C | Pyrococcus furiosus | |
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