Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0056 - 0.74
L-ethionine
0.0023 - 3.3
L-methionine
2.6
L-methionine methyl esther
-
-
0.3
MgATP2-
-
ATP in form of MgATP2-
0.39 - 2.4
S-(-)-methioninol
0.0013 - 0.026
tripolyphosphate
additional information
additional information
-
0.002
ATP
-
beta form from liver
0.005
ATP
-
alpha form from liver
0.01
ATP
pH 8, 37°C, mutant enzyme I303V/I65V/L186V
0.01
ATP
pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K
0.0145
ATP
pH 8.0, 65°C, cosubstrate: L-methionine
0.026
ATP
G6 mutant, S-adenosylmethionine synthesis
0.045
ATP
RLL and G8 mutants, S-adenosylmethionine synthesis
0.05
ATP
pH 8, 37°C, mutant enzyme I303V/I65V
0.0592
ATP
pH 8.0, 65°C, cosubstrate: L-ethionine
0.065
ATP
-
S-adenosylmethionine synthetase A
0.0686
ATP
pH 8.0, 37°C, cosubstrate: L-methionine
0.069
ATP
G7 mutants, S-adenosylmethionine synthesis
0.073
ATP
wild-type S-adenosylmethionine synthesis
0.083
ATP
wild-type, pH 8.0
0.087
ATP
G5 mutant S-adenosylmethionine synthesis
0.0969
ATP
pH 8.0, 37°C, cosubstrate: L-ethionine
0.1
ATP
mutant W387F/Y170W, 55°C
0.1
ATP
mutant W387F/Y226W, 55°C
0.1
ATP
pH 8, 37°C, mutant enzyme I303V
0.11
ATP
mutant W387F, 55°C
0.11
ATP
mutant W387F/Y371W, 55°C
0.11
ATP
mutant W387F/Y72W, 55°C
0.13
ATP
mutant D107C, pH 8.0
0.13
ATP
mutant G105C, pH 8.0
0.13
ATP
mutant W387F/Y120W, 55°C
0.156
ATP
-
S-adenosylmethionine synthetase B
0.17
ATP
pH 8, 37°C, wild-type enzyme
0.176
ATP
pH 8.4, 37°C, mutant enzyme
0.18
ATP
mutant G105R1, pH 8.0
0.18
ATP
mutant W387F/Y49W, 55°C
0.19
ATP
mutant W387F/Y255W, 55°C
0.2
ATP
mutant W387F/Y233W, 55°C
0.2
ATP
mutant W387F/Y267W, 55°C
0.206
ATP
pH 8.4, 37°C, mutant enzyme
0.21
ATP
mutant W387F/Y85W, 55°C
0.22
ATP
-
saturated with KCl
0.22
ATP
mutant W387F/Y323W, 55°C
0.22
ATP
mutant W387F/Y344W, 55°C
0.24
ATP
mutant W387F/Y273W, 55°C
0.34
ATP
-
37°C, pH 8.0, mutant enzyme I303V
0.43
ATP
-
37°C, pH 8.0, wild-type enzyme
0.493
ATP
mutant C35S, presence of dithiothreitol
0.53
ATP
wild-type, presence of glutathione
0.588
ATP
wild-type, presence of dithiothreitol
0.631
ATP
at pH 8.0 and 35°C
0.68
ATP
pH 8.4, 37°C, mutant enzyme
0.778
ATP
mutant C35S, presence of glutathione
0.92
ATP
in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C
1.72
ATP
-
at pH 8.5 and 45°C
2.07
ATP
at pH 8.5 and 35°C
2.54
ATP
mutant C61S, presence of glutathione
2.84
ATP
-
at pH 8.5 and 40°C
3.25
ATP
mutant C61S, presence of dithiothreitol
4.19
ATP
at pH 8.0 and 70°C
6.54
ATP
-
at pH 8.0 and 37°C
0.0056
L-ethionine
pH 8.0, 37°C
0.007
L-ethionine
pH 8.0, 65°C
0.0023
L-methionine
pH 8.0, 37°C
0.0028
L-methionine
pH 8.0, 65°C
0.0061
L-methionine
-
pH 7.0, 37°C
0.0075
L-methionine
-
erythrocyte extract, alpha and beta subunit
0.01
L-methionine
-
isoenzyme A
0.01
L-methionine
-
S-adenosylmethionine synthetase A
0.0125
L-methionine
-
erythrocyte extract, alpha subunit
0.015
L-methionine
-
endogenous MAT II
0.016
L-methionine
-
alpha2-transfected MAT II, two kinetic forms
0.017
L-methionine
-
alpha form from liver
0.02
L-methionine
-
isoenzyme B
0.02
L-methionine
-
recombinant MAT II co-expressing alpha2 and beta subunits
0.022
L-methionine
-
crude extract
0.022
L-methionine
one kinetic form of alpha-two subunit in the presence of beta subunit
0.022 - 0.024
L-methionine
-
ro subunit
0.024
L-methionine
-
wild-type
0.024
L-methionine
-
S-adenosylmethionine synthetase B
0.03
L-methionine
mutant D249N, pH 8.0
0.03 - 0.038
L-methionine
-
one kinetic form of alpha subunit in the presence of beta subunit
0.031
L-methionine
pH 8.0, 80°C
0.034
L-methionine
pH 8.4, 37°C
0.036
L-methionine
mutant D166N, pH 8.0
0.038
L-methionine
mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.04
L-methionine
wild-type, pH 8.0
0.041
L-methionine
-
MAT-I isoenzyme
0.041
L-methionine
mutant K280A, pH 8.0
0.043
L-methionine
pH 8.4, 37°C, mutant enzyme
0.045
L-methionine
mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.046
L-methionine
pH 8.4, 37°C, mutant enzyme
0.047
L-methionine
pH 8.0, 37°C, mutant enzyme Q113D
0.05
L-methionine
mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.051
L-methionine
pH 8.4, 37°C, mutant enzyme
0.06
L-methionine
-
uninduced E. coli NM522 strain extract alpha subunit at low L-methionine concentrations
0.06 - 0.1
L-methionine
alpha-two subunit
0.065 - 0.08
L-methionine
-
alpha subunit at low L-methionine concentrations
0.074
L-methionine
mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.075
L-methionine
-
alpha2-transfected MAT II, two kinetic forms
0.076
L-methionine
one kinetic form of alpha-two subunit in the presence of beta subunit
0.078
L-methionine
pH 8.0, 37°C, mutant enzyme K289L
0.08
L-methionine
-
S283T mutant
0.08
L-methionine
-
induced E. coli NM522 strain extract alpha subunit at low L-methionine concentrations and uninduced E. coli NM522 strain extract alpha subunit at high L-methionine concentrations
0.08
L-methionine
pH 8, 37°C, wild-type enzyme
0.08 - 0.09
L-methionine
-
alpha subunit at high L-methionine concentrations. Also one kinetic form of alpha subunit in the presence of beta subunit
0.088
L-methionine
-
Q113A mutant, S-adenosylmethionine synthesis
0.092
L-methionine
wild-type, S-adenosylmethionine synthesis
0.096
L-methionine
-
pH 8.2, 37°C
0.11
L-methionine
pH 8.0, 37°C
0.11
L-methionine
wild-type, pH 8.0
0.12
L-methionine
-
pH 8.0, 37°C
0.12
L-methionine
-
37°C, pH 8.0, mutant enzyme I303V
0.13
L-methionine
mutant W387F/Y323W, 55°C
0.13
L-methionine
mutant W387F/Y72W, 55°C
0.137
L-methionine
mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.137
L-methionine
mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.14
L-methionine
pH 8.0, 37°C, wild-type enzyme
0.14
L-methionine
pH 8.0, 58°C
0.141
L-methionine
mutant D282N, pH 8.0
0.147
L-methionine
mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.17
L-methionine
mutant K256A, pH 8.0
0.17
L-methionine
mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.17
L-methionine
pH 8, 37°C, mutant enzyme I303V/I65V/L186V/N104K
0.176
L-methionine
mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.18
L-methionine
mutant D107R1, pH 8.0
0.18
L-methionine
-
37°C, pH 8.0, wild-type enzyme
0.19
L-methionine
mutant D107R1, pH 8.0
0.2
L-methionine
-
induced E. coli NM522 strain extract alpha subunit at high L-methionine concentrations
0.215
L-methionine
-
MAT-III isoenzyme
0.22
L-methionine
-
wild-type
0.23
L-methionine
RLL mutant, S-adenosylmethionine synthesis
0.25
L-methionine
mutant W387F/Y371W, 55°C
0.26
L-methionine
in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C
0.287
L-methionine
wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0
0.288
L-methionine
37°C, pH 8.2
0.29
L-methionine
mutant D107C, pH 8.0
0.3
L-methionine
G6 mutant, S-adenosylmethionine synthesis
0.3
L-methionine
-
saturated with KCl
0.3
L-methionine
mutant W387F/Y226W, 55°C
0.3
L-methionine
mutant W387F/Y273W, 55°C
0.3
L-methionine
pH 8, 37°C, mutant enzyme I303V/I65V/L186V
0.31
L-methionine
-
at pH 8.0 and 37°C
0.31
L-methionine
mutant W387F, 55°C
0.31
L-methionine
wild-type, 55°C
0.33
L-methionine
mutant W387F/Y344W, 55°C
0.42
L-methionine
pH 8, 37°C, mutant enzyme I303V
0.43
L-methionine
pH 8, 37°C, mutant enzyme I303V/I65V
0.45
L-methionine
mutant G105C, pH 8.0
0.45
L-methionine
mutant W387F/Y267W, 55°C
0.47
L-methionine
mutant W387F/Y233W, 55°C
0.49
L-methionine
G7 mutant, S-adenosylmethionine synthesis
0.5
L-methionine
mutant W387F/Y255W, 55°C
0.51
L-methionine
at pH 8.5 and 35°C
0.527
L-methionine
at pH 8.0 and 35°C
0.54
L-methionine
mutant W387F/Y120W, 55°C
0.57
L-methionine
mutant W387F/Y85W, 55°C
0.62
L-methionine
G8 mutant, S-adenosylmethionine synthesis
0.66
L-methionine
mutant W387F/Y49W, 55°C
0.75
L-methionine
mutant G105R1, pH 8.0
0.77
L-methionine
G5 mutant, S-adenosylmethionine synthesis
0.85
L-methionine
-
at pH 8.5 and 45°C
1
L-methionine
mutant W387F/Y170W, 55°C
1.2
L-methionine
at pH 8.0 and 70°C
1.47
L-methionine
-
at pH 8.5 and 40°C
0.0083
methionine
-
MAT-II isoenzyme
0.222
methionine
mutant C35S, presence of dithiothreitol
0.246
methionine
wild-type, presence of dithiothreitol
0.449
methionine
mutant C35S, presence of glutathione
0.5
methionine
-
beta form from liver
0.756
methionine
mutant C61S, presence of dithiothreitol
0.794
methionine
mutant C61S, presence of glutathione
1.12
methionine
wild-type, presence of glutathione
0.006
Mg2+
-
alpha form from liver
0.007
Mg2+
-
beta form from liver
0.39
S-(-)-methioninol
pH 8.0, 37°C, mutant enzyme Q113D
1
S-(-)-methioninol
pH 8.0, 37°C, mutant enzyme K289L
2.4
S-(-)-methioninol
pH 8.0, 37°C, wild-type enzyme
0.0013
tripolyphosphate
wild type, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.0015
tripolyphosphate
G7 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.0016
tripolyphosphate
G8 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.003
tripolyphosphate
wild type, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.0032
tripolyphosphate
G6 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.0053
tripolyphosphate
G5 mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.008
tripolyphosphate
G8 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.011
tripolyphosphate
G6 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.014
tripolyphosphate
RLL mutant, tripolyphosphatase activity, in absence of S-adenosylmethionine
0.015
tripolyphosphate
G7 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.024
tripolyphosphate
G5 mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
0.026
tripolyphosphate
RLL mutant, tripolyphosphatase activity, in the presence of 0.1 mM of S-adenosylmethionine
additional information
additional information
-
high performance liquid chromatography assay method using catechol-O-methyltransferase-coupled fluorometric detection, negative cooperativity of enzyme with Hill coefficinet of 0.5
-
additional information
additional information
-
different isoforms of MAT differ in kinetic and regulatory properties, overview
-