Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
Crystallization (Comment) | Organism |
---|---|
using 50 mM Tris-HCl pH 8.0, 200 mM KCl, 2% (w/v) ethylene glycol, 1 mM dithiothreitol, 12-16% (w/v) polyethylene glycol 8000 | Campylobacter jejuni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme requires two divalent metal ions in the active site | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Campylobacter jejuni | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-IMAC column chromatography and Source 30Q column chromatography | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Campylobacter jejuni | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Campylobacter jejuni |
Synonyms | Comment | Organism |
---|---|---|
AdoMet synthetase | - |
Campylobacter jejuni |
MAT | - |
Campylobacter jejuni |