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Literature summary for 2.5.1.6 extracted from

  • Zano, S.P.; Pavlovsky, A.G.; Viola, R.E.
    Structure of an unusual S-adenosylmethionine synthetase from Campylobacter jejuni (2014), Acta Crystallogr. Sect. D, 70, 442-450.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment) Organism
using 50 mM Tris-HCl pH 8.0, 200 mM KCl, 2% (w/v) ethylene glycol, 1 mM dithiothreitol, 12-16% (w/v) polyethylene glycol 8000 Campylobacter jejuni

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme requires two divalent metal ions in the active site Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Campylobacter jejuni
-
phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IMAC column chromatography and Source 30Q column chromatography Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Campylobacter jejuni phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Subunits

Subunits Comment Organism
homodimer
-
Campylobacter jejuni

Synonyms

Synonyms Comment Organism
AdoMet synthetase
-
Campylobacter jejuni
MAT
-
Campylobacter jejuni