Activating Compound | Comment | Organism | Structure |
---|---|---|---|
S-adenosylmethionine | nonessential activator of tripolyphosphatase activity in the range of 0.005-0.100 mM | Leishmania donovani |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli XL-1Blue strain | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | time-dependent inactivation of both MAT activities | Leishmania donovani | |
S-adenosylmethionine | non competitive inhibition | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
MAT II, gel filtration | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q9U6Q8 | - |
- |
Renatured (Comment) | Organism |
---|---|
refolding by a fourfold dilution refolding buffer up to a concentration of 2 M urea | Leishmania donovani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.058 | - |
tripolyphosphase activity | Leishmania donovani |
0.2 | - |
S-adenosylmethionine synthesis activity | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Leishmania donovani | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
tripolyphosphate + H2O | - |
Leishmania donovani | diphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
tripolyphosphate | tripolyphosphase activity | Leishmania donovani | |
0.032 | - |
S-adenosylmethionine | S-adenosylmethionine synthesis activity | Leishmania donovani |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
S-adenosylmethionine | ATP concentrations of 0.5-5 mM | Leishmania donovani | |
4 | - |
S-adenosylmethionine | L-methionine concentrations of 0.5-5 mM | Leishmania donovani |