Information on EC 2.7.13.3 - histidine kinase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY
2.7.13.3
protein-histidine kinases without information on stereospecificity towards histidine
RECOMMENDED NAME
GeneOntology No.
histidine kinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phospho group transfer
-
-
-
-
phospho-group transfer
Q88PG3
-
SYSTEMATIC NAME
IUBMB Comments
ATP:protein-L-histidine N-phosphotransferase
This entry has been included to accommodate those protein-histidine kinases for which the phosphorylation site has not been established (i.e. either the pros- or tele-nitrogen of histidine). A number of histones can act as acceptor.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
adaptive-response sensory-kinase A
-
-
aerobic respiration control sensor protein arcB
P0AEC3
-
aerobic respiration control sensor protein arcB
P58363
-
aerobic respiration control sensor protein arcB
Q8FD66
-
aerobic respiration control sensor protein arcB
Q8FD66
-
-
aerobic respiration control sensor protein arcB homolog
P44578
-
aerobic respiration control sensor/response regulatory protein
-
-
alginate biosynthesis sensor protein KINB
O34206
-
AlHK1p
Q09JB2, Q09JB3, Q09JB4, Q09JB6, Q09JB7, Q09JB8, Q09JB9
-
alkaline phosphatase synthesis sensor protein phoR
P23545
-
alkaline phosphatase synthesis sensor protein phoR
Q8YE51
-
alkaline phosphatase synthesis sensor protein phoR
Q898N3
-
alrO117
-
-
AtoS-AtoC two-component signal transduction system
-
-
autolysin sensor kinase
Q8R6B2
-
BA2291
Q81QX4
-
BA2291
Bacillus anthracis 34F2DELTA118
Q81QX4
-
-
bacteriophytochrome
Q9HWR3
-
bacteriophytochrome (phytochrome-like protein)
Q9RZA4
-
BarA protein
P0AEC7
-
BarA sensor kinase (sensory histidine kinase)
Q9L9E4
-
BOS1
Botryotinia fuckeliana UWS111
-
-
-
C4-dicarboxylate transport sensor protein dctB
P10047
-
C4-dicarboxylate transport sensor protein dctB
P13633
-
C4-dicarboxylate transport sensor protein dctS
P37739
-
chemotaxis histidine kinase
-
-
chemotaxis protein
Q8PX97, Q8Q010
-
chemotaxis protein cheA
P29072
-
chemotaxis protein cheA
Q44737
-
chemotaxis protein cheA
Q893S8
-
chemotaxis protein cheA
Q8FGP1
-
chemotaxis protein cheA
Q8FGP1
-
-
chemotaxis protein cheA
Q92DW2
-
chemotaxis protein cheA
Q48768
-
chemotaxis protein cheA
Q53135
-
chemotaxis protein cheA
Q52880
-
chemotaxis protein cheA
Q56310
-
chemotaxis protein cheA
P96123
-
chemotaxis protein CheA (sensory transducer kinase)
-
-
chemotaxis-specific histidine autokinase CheA
P07363
-
CheN
P29072
-
Chk1p
Candida albicans REP36-1
-
-
-
CinS
Q88KY2
-
CitAP
P52687
recombinant sensory domain of CitA
class III HK
-
-
class III HK
Botryotinia fuckeliana UWS111
-
-
-
copper resistance, histidine kinase
Q8ZQ54
-
cyanobacterial phytochrome A
Q9LCC2
-
cyanobacterial phytochrome B
Q9R6X3
-
cytokinin-independent1
-
-
DctB
Vibrio cholerae MO45
-
-
-
DcuS
P0AEC8
-
DesK
O34757
-
drug sensory protein A
P20169
-
ENVZ
P0AEJ4
-
EspA
Q95434
-
ethanolamine two-component sensor kinase
Q8R6C3
-
ethylene receptor
O49230
-
ethylene receptor (CS-ETR1)
Q9SSY6
-
ethylene receptor (MEETR1) (Cm-ETR1)
O82436
-
ethylene receptor (PE-ETR1)
Q9ZWL6
-
ethylene receptor 1 (LeETR1)
Q41342
-
ethylene receptor 2 (LeETR2)
O49187
-
ethylene receptor 2 (PhETR2)
Q9XH57
-
ethylene receptor12 (PhETR2)
Q9XH58
-
ethylene resistant 1
-
the carboxyl-terminal part of ethylene resistant 1 consists of a histidine kinase domain and a response regulator domain
ETR1 ethylene receptor
-
-
FlgS
Q25026
-
FrzE
-
hybrid protein consisting of a CheA histidine kinase and a CheY receiver domain
gliding motility regulatory protein
P18769
-
group III two-component histidine kinase
Q09JB2, Q09JB3, Q09JB4, Q09JB6, Q09JB7, Q09JB8, Q09JB9
-
heme-nitric oxide oxygen binding proteins-associated histidine kinase
-
-
HHK1
Penicillium marneffei
-
-
high-affinity potassium transport system
Q8ZQW4
-
Hik1
Q9C1U1
-
His kinase
-
-
HISTIDIN kinase (histidine protein kinase PlnB, sensor protein)
Q48828
-
HISTIDIN kinase (histidine protein kinase PlnB, sensor protein)
Lactobacillus plantarum C11
Q48828
-
-
histidine autokinase CheA
P07363
-
histidine kinase
-
-
histidine kinase
Botryotinia fuckeliana UWS111
-
-
-
histidine kinase
-
-
histidine kinase
Q57BR6
-
histidine kinase
Brucella abortus XDB1104
Q57BR6
-
-
histidine kinase
-
-
histidine kinase
-
-
histidine kinase
-
-
histidine kinase
Pseudomonas syringae DC3000
-
-
-
histidine kinase
Q8DN03, Q8DN65, Q8DNC1, Q8DNX7, Q8DPL8, Q8DQN7, Q8DR46, Q8DRK0
-
histidine kinase
Q9F2F5
-
histidine kinase
-
-
histidine kinase
Q9X180
-
histidine kinase BA1351
-
-
histidine kinase BA1356
-
-
histidine kinase BA1478
-
-
histidine kinase BA2291
-
-
histidine kinase BA2636
-
-
histidine kinase BA2644
-
-
histidine kinase BA3702
-
-
histidine kinase BA4223
-
-
histidine kinase BA5029
-
-
histidine kinase CikA
-
-
histidine kinase CKI1
-
-
histidine kinase DivJ
-
-
histidine kinase EnvZ
-
-
histidine kinase Hik10
-
-
histidine kinase Hik16
-
-
histidine kinase Hik33
-
-
histidine kinase Hik34
-
-
histidine kinase Hik41
-
-
histidine kinase PilS
-
-
histidine kinase PleC
-
-
histidine kinase Ppr
-
-
histidine kinase SasA
-
-
histidine kinase SasA
Synechococcus elongatus PCC7942
-
-
-
histidine kinase2
-
-
histidine kinase3
-
-
histidine protein kinase
-
-
histidine protein kinase
-
-
histidine protein kinase
Q8DMW4
-
histidine protein kinase
-
-
histidine protein kinase
Xanthomonas oryzae pv. oryzae PXO99
-
-
-
histidine protein kinase KinB
O34206
-
histidine protein kinase, sensor protein
-
-
histidine sensor kinase
Q88KY2
-
HK cytokinin-independent1
-
-
HP0244
Q25026
-
hybrid histidine kinase
-
-
HydH
Q9APE0
-
iron-sensing histidine kinase
-
-
iron-sensing histidine kinase
Streptomyces reticuli Tue54
-
;
-
KINA
P16497
-
KINA
Bacillus subtilis 168
-
-
-
KINA
Bacillus subtilis 1A40
P16497
-
-
LeETR1
Q41342
-
light sensor histidine kinase
-
-
limited host range virA protein(LHR virA)
P07167
-
LuxQ
P54302
-
multidomain membrane sensor kinase
P0AEC8
-
NarX
P0AFA2
-
Ni2+-sensing histidine kinase NrsS
-
-
NikA
Emericella nidulans BPU1
-
-
-
nisin biosynthesis sensor protein nisK
P42707
-
nitrate-nitrite sensor protein
Q9ZC64
-
nitrate-responsive histidine kinase
P0AFA2
-
nitrate/nitrite sensor protein
Q9ZC64
-
nitrate/nitrite sensor protein narQ
P27896
-
nitrate/nitrite sensor protein narQ
Q8FF85
-
nitrate/nitrite sensor protein narQ
Q8FF85
-
-
nitrate/nitrite sensor protein narQ
Q8Z4S5
-
nitrate/nitrite sensor protein narX
-
-
nitrate/nitrite sensor protein narX
Q8FHZ2
-
nitrate/nitrite sensor protein narX
Q8FHZ2
-
-
nitrogen regulation protein NR(II)
P0AFB6
-
nitrogen regulation protein NR(II)
Q8FBG6
-
nitrogen regulation protein NR(II)
Q8FBG6
-
-
nitrogen regulation protein NR(II)
P28788
-
nitrogen regulation protein NR(II)
P0A2D9
-
nitrogen regulation protein ntrB
P45670
-
nitrogen regulation protein ntrB
P10578
-
nitrogen regulation protein ntrB
P10578
-
-
nitrogen regulation protein ntrB
Q8YHD6
-
nitrogen regulation protein ntrB
P06218
-
nitrogen regulation protein ntrB
P41503
-
nitrogen regulation protein ntrB
P09431
-
nitrogen regulation protein ntrB
Q52977
-
nitrogen regulation protein ntrB
P19906
-
nitrogen regulation protein ntrY
Q04850
-
nitrogen regulation protein ntrY
Q8YHD4
-
nitrogen regulation protein ntrY homolog
P45675
-
nodulation protein V
P15939
-
nodulation protein V
Bradyrhizobium japonicum USDA110
P15939
-
-
NodV protein
P15939
-
NreB
Staphylococcus carnosus M1
-
-
-
ornithine decarboxylase antizyme
Q06067
-
osmolarity sensor protein (protein histidine)
-
-
osmolarity sensor protein envZ
Q8YIF2
-
osmolarity sensor protein envZ
Q8FCU0
-
osmolarity sensor protein envZ
Q8FCU0
-
-
osmolarity two-component system protein SLN1
P39928
-
osmosensor group III histidine kinase
Q66WP9
-
PaBphP-PCD
Q9HWR3
-
PdhS
Q57BR6
-
PdhS
Brucella abortus XDB1104
Q57BR6
-
-
PhoR1
Q9Z5F5
-
phosphate regulon sensor protein phoR
Q8YF98
-
phosphate regulon sensor protein phoR
Q895Y4
-
phosphate regulon sensor protein phoR
P08400
-
phosphate regulon sensor protein phoR
Q8FKD0
-
phosphate regulon sensor protein phoR
Q8FKD0
-
-
phosphate regulon sensor protein phoR
P71380
-
phosphate regulon sensor protein phoR
P45608
-
phosphate regulon sensor protein phoR
P23621
-
phosphate regulon sensor protein phoR
P45609
-
phosphate regulon sensor protein phoR
-
-
phosphate-sensing histidine kinase SphS
-
-
phosphoglycerate transport system sensor protein pgtB
P37433
-
photoactive yellow protein phytochrome-related protein
-
-
phytochrome A
-
-
phytochrome-like protein cph1 (light-regulated histidine)
Q55168
-
PleC-DivJ homolog sensor
Q57BR6
-
PleC-DivJ homolog sensor
Brucella abortus XDB1104
Q57BR6
-
-
PleCHKD
Q2GJD7
the PleC kinase domain has histidine kinase activity
positive and negative sensor protein for pho regulon
-
-
PYP phytochrome-related protein
-
-
RaxH
Xanthomonas oryzae pv. oryzae PXO99
-
-
-
receptor histidine kinase
-
-
SaeS
Staphylococcus aureus Newman
Q840P7
-
-
SasA
Synechococcus elongatus PCC7942
-
-
-
secretion system regulator:sensor component
Q8ZPP5
-
SenS
Streptomyces reticuli Tue54
-
;
-
sensor histidine kinase
A0KND7
-
sensor histidine kinase
Q81QX4
-
sensor histidine kinase
Bacillus anthracis 34F2DELTA118
Q81QX4
-
-
sensor histidine kinase
-
-
sensor histidine kinase
Q45614
-
sensor histidine kinase
-
-
sensor histidine kinase
P52687
-
sensor histidine kinase
-
-
sensor histidine kinase
Q88PG3
two-component system formed by sensor histidine kinase and a response regulator
sensor histidine kinase
Q840P7
-
sensor histidine kinase
Staphylococcus aureus Newman
Q840P7
-
-
sensor histidine kinase A
-
-
sensor histidine kinase A
Bacillus subtilis 168
-
-
-
sensor histidine kinase mtrB
Q9CCV1
-
sensor histidine kinase mtrB
Q50496
-
sensor histidine kinase regB (PrrB protein)
Q3J6C1
-
sensor kinase
Q2GJD7
-
sensor kinase citA
P52687
-
sensor kinase cusS
P77485
-
sensor kinase cusS
Q8FK37
-
sensor kinase cusS
Q8FK37
-
-
sensor kinase dpiB
Q894l7
-
sensor kinase dpiB
Q8FJZ9
-
sensor kinase dpiB
Q8FJZ9
-
-
sensor kinase dpiB (sensor kinase citA)
P77510
-
sensor protein afsQ2
Q04943
-
sensor protein atoS
Q06067
-
sensor protein atoS
Q8FFP8
-
sensor protein atoS
Q8FFP8
-
-
sensor protein baeS
P30847
-
sensor protein baeS
Q8FG01
-
sensor protein baeS
Q8FG01
-
-
sensor protein basS/pmrB
P30844
-
sensor protein basS/pmrB
Q8FAU6
-
sensor protein basS/pmrB
Q8FAU6
-
-
sensor protein chvG
Q07737
-
sensor protein chvG
Q8YE43
-
sensor protein chvG (histidine kinase sensory protein)
P72292
-
sensor protein ciaH
P0A4I6
-
sensor protein citS
Q9RC53
-
sensor protein citS
O34427
-
sensor protein copS
Q02541
-
sensor protein creC
P08401
-
sensor protein creC
Q8FA38
-
sensor protein creC
Q8FA38
-
-
sensor protein cssS
O32193
-
sensor protein cutS
P0A4I7
-
sensor protein czcS
Q44007
-
sensor protein dcuA
P59342
-
sensor protein dcuS
P59340
-
sensor protein dcuS
P59340
-
-
sensor protein dcuS
P59341
-
sensor protein degS
P13799
-
sensor protein degS
P54663
-
sensor protein degS
Q8YFD9
-
sensor protein divL
Q9RQQ9
-
sensor protein evgS precursor
P30855
-
sensor protein evgS precursor
P58402
-
sensor protein evgS precursor
Q8CVU5
-
sensor protein evgS precursor
Q8CVU5
-
-
sensor protein fixL
P26489
-
sensor protein fixL
P23222
-
sensor protein fixL
P10955
-
sensor protein for basR
-
-
sensor protein gacS
P48027
-
sensor protein irlS
O31396
-
sensor protein kdpD
P94608
-
sensor protein kdpD
P21865
-
sensor protein kdpD
Q8FJV6
-
sensor protein kdpD
Q8FJV6
-
-
sensor protein kdpD
P96372
-
sensor protein kinase (sensor protein PhoQ)
-
-
sensor protein luxN
P0C5S6
-
sensor protein luxQ
P54302
-
sensor protein narQ homolog
P44604
-
sensor protein pfeS
Q04804
-
sensor protein phoQ
Q8YG26
-
sensor protein phoQ
P23837
-
sensor protein phoQ
Q8FIB8
-
sensor protein phoQ
Q8FIB8
-
-
sensor protein qseC
P40719
-
sensor protein qseC
Q8FDJ7
-
sensor protein qseC
Q8X524
-
sensor protein qseC
Q8FDJ7
-
-
sensor protein qseC
P45336
-
sensor protein rcsC
Q8FFP9
-
sensor protein rcsC
Q8FFP9
-
-
sensor protein rcsC (capsular synthesis regulator)
P14376
-
sensor protein rcsC (capsular synthesis regulator)
Escherichia coli O9:K30:H12
P14376
-
-
sensor protein rcsC (capsular synthesis regulator)
Q56128
-
sensor protein rcsC (capsular synthesis regulator)
P58662
-
sensor protein resE
P35164
-
sensor protein resE
Q893B1, Q893K0, Q894W4
-
sensor protein roxS
Q88PG3
-
sensor protein rprX
Q08408
-
sensor protein rstB
P18392
-
sensor protein rstB
Q8FHA9
-
sensor protein rstB
Q8FHA9
-
-
sensor protein sphS
P39664
-
sensor protein torS
P58356
-
sensor protein torS
Q8FJ55
-
sensor protein torS
Q8FJ55
-
-
sensor protein uhpB
P09835
-
sensor protein uhpB
Q8FBX4
-
sensor protein uhpB
Q8FBX4
-
-
sensor protein vanS (vancomycin resistance protein vanS)
Q06240
-
sensor protein vanSB (vancomycin B-type resistance)
Q47745
-
sensor protein yycG
Q45614
-
sensor protein zraS
P14377
-
sensor protein zraS
Q8FB70
-
sensor protein zraS
Q8X614
-
sensor protein zraS
Q8FB70
-
-
sensor protein zraS
Q9APE0
-
sensor with histidine kinase
-
-
sensor with histidine kinase
Emericella nidulans ABPU1
-
-
-
sensor-like histidine kinase senX3
P0A601
-
sensor-like histidine kinase senX3
P54883
-
sensor-like histidine kinase senX3
P0A600
-
sensory histidine kinase
-
-
sensory histidine kinase
-
-
sensory histidine kinase
-
-
sensory histidine kinase
-
-
sensory histidine kinase in two-component regulatory system with ArcA
Q8ZLR5
-
sensory histidine kinase in two-component regulatory system with DcuR, senses fumarate/C4-dicarboxylate
Q8ZKD8
-
sensory histidine kinase in two-component regulatory system with NarP
Q8ZN78, Q8ZP35, Q8ZPL6
-
sensory HK
-
-
sensory kinase (alternative) in two-component regulatory system with CreB (or alternatively PhoB), senses catabolite repression
Q8ZJU5
-
sensory kinase in multi-component regulatory system with TorR
Q8ZKZ3
-
sensory kinase in two-component regulatory system with CpxR, senses misfolded proteins in bacterial envelope
Q8XG60
-
sensory kinase in two-component regulatory system with PhoB, regulates pho regulon
Q8ZRE1
-
sensory kinase in two-component regulatory system wtih KdpE, regulates kdp operon
Q8ZQW4
-
sensory transduction histidine kinase
Q8YBU4, Q8YC53, Q8YDX2, Q8YFB6, Q8YG34, Q8YG37, Q8YH57, Q8YIR9
-
sensory transduction protein kinase
Q8YER2, Q8YF74
-
sensory transduction protein kinase
Q892E7, Q893C2, Q893l4, Q894P4, Q896X1, Q897D4, Q899I6, Q899l3
-
sensory transduction protein kinase
Q8R5N6, Q8R5P0
-
sensory transduction protein kinase
Q8PTR6, Q8PVN2
-
sensory/regulatory protein rpfC
P0C0F6
-
signal transduction histidine kinase
-
-
Slr1759
-
Slr1759 is a multidomain hybrid histidine kinase
sporulation histidine kinase
-
-
sporulation kinase A (stage II sporulation protein J)
P16497
-
sporulation kinase B
Q08430
-
sporulation kinase C
P39764
-
sporulation kinase C (sensor kinase)
Q8CXG3
-
subtilin biosynthesis sensor protein spaK
P33113
-
subtilin biosynthesis sensor protein spaK
Q897U6
-
subtilin biosynthesis sensor protein spaK
P33639
-
tetrathionate reductase complex: sensory transduction histidine kinase
Q8ZPP6
-
thermosensor histidine kinase
O34757
-
ThkA
Q9X180
-
TMAO reductase S
P39453
-
TMAO reductase S
-
-
TMAO reductase S
Vibrio parahaemolyticus EB101
-
-
-
TorS
P39453
sensor histidine kinase
TorS
-
; a sensor histidine kinase
TorS
Vibrio parahaemolyticus EB101
-
; a sensor histidine kinase
-
transmembrane sensor histidine kinase transcription regulator protein
Q8XVU1
-
tricarboxylic transport: regulatory protein
Q8ZMM5
-
trimethylamine-N-oxide reductase S
P39453
-
trimethylamine-N-oxide reductase S
-
-
trimethylamine-N-oxide reductase S
Vibrio parahaemolyticus EB101
-
-
-
TWO component response regulator transcription regulator protein
Q8XVU0
-
two component sensor kinase/response regulator protein RcsC
-
-
two component system histidine kinase
Q8R6C4
-
two component system histidine kinase
Q8PSH8, Q8PSI0, Q8PTE2, Q8PV00
-
two-component regulatory protein
-
-
two-component regulatory protein sensor kinase KdpD
-
-
two-component sensor kinase
Q8Z1N4, Q8Z1P5
-
two-component sensor kinase czcS
Q8R693
-
two-component sensor kinase yesM
Q893H6
-
two-component sensor kinase yesM
Q8R6B7
-
two-component system sensor protein
-
-
VicK
Streptococcus pneumoniae (D39)
Q9S1J9
-
-
virulence sensor protein bvgS precursor
P26762
-
virulence sensor protein bvgS precursor
P40330
-
virulence sensor protein bvgS precursor
P16575
-
VncS, histidine kinase
Q8DQR9
-
wide host range virA protein (WHR virA)
P07168
-
wide host range virA protein (WHR virA)
P10799
-
wide host range virA protein (WHR virA)
P18540
-
wide host range virA protein (WHR virA)
Agrobacterium tumefaciens 15955
P10799
-
-
YycG
Q45614
-
methanol utilization control sensor protein moxY
P29905
-
additional information
-
AHK5 belongs to the the canonical HK class of proteins in Arabidopsis thaliana
additional information
P0AEC8
DcuS is a member of the periplasmic sensing histidine kinases
CAS REGISTRY NUMBER
COMMENTARY
99283-67-7
protein-histidine kinases, EC 2.7.13.1, EC 2.7.13.2, and EC 2.7.13.3 are not distinguished in Chemical Abstracts
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
histidine kinase AtBphP1; histidine kinase AtBphP2
-
-
Manually annotated by BRENDA team
strain 15955
SwissProt
Manually annotated by BRENDA team
strain C58 /ATCC 33970
SwissProt
Manually annotated by BRENDA team
strain C58 /ATCC 33970
SwissProt
Manually annotated by BRENDA team
Agrobacterium tumefaciens 15955
strain 15955
SwissProt
Manually annotated by BRENDA team
strains Abra40 and Abra363H5M
UniProt
Manually annotated by BRENDA team
19 different isolates, gene AlHK1
UniProt
Manually annotated by BRENDA team
PCC 7120
-
-
Manually annotated by BRENDA team
strain PCC 7120
SwissProt
Manually annotated by BRENDA team
histidine kinase BA1351; histidine kinase BA1356; histidine kinase BA1478; histidine kinase BA2291; histidine kinase BA2636; histidine kinase BA2644; histidine kinase BA3702; histidine kinase BA4223; histidine kinase BA5029
-
-
Manually annotated by BRENDA team
strain 34F2DELTA118, gene BA2291, the organism contains nine genes encoding putative sporulation histidine kinases
UniProt
Manually annotated by BRENDA team
Bacillus anthracis 34F2DELTA118
strain 34F2DELTA118, gene BA2291, the organism contains nine genes encoding putative sporulation histidine kinases
UniProt
Manually annotated by BRENDA team
strain 168
-
-
Manually annotated by BRENDA team
strain 1A40
SwissProt
Manually annotated by BRENDA team
wild-type strain JH642 and conditional ftsZ expression strain KP444, gene yycG
SwissProt
Manually annotated by BRENDA team
Bacillus subtilis 168
strain 168
-
-
Manually annotated by BRENDA team
Bacillus subtilis 1A40
strain 1A40
SwissProt
Manually annotated by BRENDA team
UWS111 strain
-
-
Manually annotated by BRENDA team
Botryotinia fuckeliana UWS111
UWS111 strain
-
-
Manually annotated by BRENDA team
strain USDA110
SwissProt
Manually annotated by BRENDA team
Bradyrhizobium japonicum USDA110
strain USDA110
SwissProt
Manually annotated by BRENDA team
strain RP501 parasponia
SwissProt
Manually annotated by BRENDA team
strain RP501 parasponia
SwissProt
Manually annotated by BRENDA team
all strains derived from Brucella abortus 544 NalR, XDB1104 strain
SwissProt
Manually annotated by BRENDA team
Brucella abortus XDB1104
all strains derived from Brucella abortus 544 NalR, XDB1104 strain
SwissProt
Manually annotated by BRENDA team
strain REP36-1
-
-
Manually annotated by BRENDA team
Candida albicans REP36-1
strain REP36-1
-
-
Manually annotated by BRENDA team
histidine kinase DivJ; histidine kinase PleC
-
-
Manually annotated by BRENDA team
strain ATCC 13032
-
-
Manually annotated by BRENDA team
var. reticulatus
SwissProt
Manually annotated by BRENDA team
; strain ABPU1
-
-
Manually annotated by BRENDA team
strain BPU1
-
-
Manually annotated by BRENDA team
Emericella nidulans ABPU1
strain ABPU1
-
-
Manually annotated by BRENDA team
Emericella nidulans BPU1
strain BPU1
-
-
Manually annotated by BRENDA team
strain V583, a clinical isolate from humans
-
-
Manually annotated by BRENDA team
strains ATCC 29212 and ATCC 51299
-
-
Manually annotated by BRENDA team
Enterococcus faecium BM4147
BM4147
SwissProt
Manually annotated by BRENDA team
-
P14376
SwissProt
Manually annotated by BRENDA team
K-12
SwissProt
Manually annotated by BRENDA team
strain K-12
SwissProt
Manually annotated by BRENDA team
strain K-12
-
-
Manually annotated by BRENDA team
strain K-12
UniProt
Manually annotated by BRENDA team
strain O157:H7
SwissProt
Manually annotated by BRENDA team
strain O9:K30:H12
P14376
SwissProt
Manually annotated by BRENDA team
strains ATCC 25922 and ATCC 43300
-
-
Manually annotated by BRENDA team
gene dcuS
UniProt
Manually annotated by BRENDA team
Escherichia coli O9:K30:H12
strain O9:K30:H12
P14376
SwissProt
Manually annotated by BRENDA team
gene HP0244
UniProt
Manually annotated by BRENDA team
strain 26695
-
-
Manually annotated by BRENDA team
strain J99
-
-
Manually annotated by BRENDA team
Lactobacillus plantarum C11
strain C11
SwissProt
Manually annotated by BRENDA team
subsp. lactis
SwissProt
Manually annotated by BRENDA team
-
P54883, Q9CCV1
SwissProt
Manually annotated by BRENDA team
wild-type strain DZ2, gene espA
UniProt
Manually annotated by BRENDA team
var. Xanthi, histidine kinase NTHK2
Uniprot
Manually annotated by BRENDA team
strain PCC 73102
-
-
Manually annotated by BRENDA team
Penicillium marneffei
-
-
-
Manually annotated by BRENDA team
strain ATCC 27853
-
-
Manually annotated by BRENDA team
sensor protein, the two components are encoded by PP_0887 (roxS, sensor protein) and PP_0888 (roxR, response regulator) which are transcribed in a single unit.; strain KT2440
UniProt
Manually annotated by BRENDA team
strain KT2440
UniProt
Manually annotated by BRENDA team
pv. syringae
SwissProt
Manually annotated by BRENDA team
strain DC3000
-
-
Manually annotated by BRENDA team
Pseudomonas syringae DC3000
strain DC3000
-
-
Manually annotated by BRENDA team
biovar phaseoli
SwissProt
Manually annotated by BRENDA team
strain ATCC 29213
-
-
Manually annotated by BRENDA team
strain Newman
UniProt
Manually annotated by BRENDA team
Staphylococcus aureus Newman
strain Newman
UniProt
Manually annotated by BRENDA team
Staphylococcus carnosus M1
strain M1
-
-
Manually annotated by BRENDA team
strain (D39) ATCC 7466
UniProt
Manually annotated by BRENDA team
strain ATCC BAA-255/R6
SwissProt
Manually annotated by BRENDA team
Streptococcus pneumoniae (D39)
strain (D39) ATCC 7466
UniProt
Manually annotated by BRENDA team
strain A3(2) (ATCC BAA471D)
-
-
Manually annotated by BRENDA team
strain Tue54, gene senS
-
-
Manually annotated by BRENDA team
strain Tue54; strain Tue54, gene senS
-
-
Manually annotated by BRENDA team
Streptomyces reticuli Tue54
strain Tue54, gene senS
-
-
Manually annotated by BRENDA team
Streptomyces reticuli Tue54
strain Tue54; strain Tue54, gene senS
-
-
Manually annotated by BRENDA team
PCC 7942, histidine kinase CikA
-
-
Manually annotated by BRENDA team
Synechococcus elongatus PCC7942
PCC7942
-
-
Manually annotated by BRENDA team
strain PCC 7942
SwissProt
Manually annotated by BRENDA team
PCC 6803
-
-
Manually annotated by BRENDA team
PCC 6803, histidine kinase Hik34
-
-
Manually annotated by BRENDA team
PCC6803, histidine kinase Hik10; PCC6803, histidine kinase Hik16; PCC6803, histidine kinase Hik33; PCC6803, histidine kinase Hik34; PCC6803, histidine kinase Hik41
-
-
Manually annotated by BRENDA team
strain PCC 6803
SwissProt
Manually annotated by BRENDA team
strain PCC 6803
SwissProt
Manually annotated by BRENDA team
strain PCC 6803, histidine kinase Hik16; strain PCC 6803, histidine kinase Hik34; strain PCC 6803, histidine kinase Hik41
-
-
Manually annotated by BRENDA team
strain MO45, gene dctB
-
-
Manually annotated by BRENDA team
Vibrio cholerae MO45
strain MO45, gene dctB
-
-
Manually annotated by BRENDA team
; strain EB101
-
-
Manually annotated by BRENDA team
Vibrio parahaemolyticus EB101
strain EB101
-
-
Manually annotated by BRENDA team
pv. campestris
P0C0F6
SwissProt
Manually annotated by BRENDA team
Xanthomonas oryzae pv. oryzae PXO99
strain PXO99
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
Arabidopsis mutants lacking HK5 show reduced stomatal closure in response to H2O2, overexpression of HK5 results in constitutively less stomatal closure
malfunction
-
HP0244 deletion abolishes urease activation and assembly, impairs cytoplasmic and periplasmic pH homeostasis, and depolarizes the cells, with an about 7-log loss of survival at pH 2.5, even in 10 mM urea
malfunction
-
genetic modifications of cytokinin-independent1 activity in Arabidopsis cause dysfunction of the two-component signaling pathway and defects in procambial cell maintenance, loss-of-function histidine kinase2 and histidine kinase3 mutants show defects in procambium proliferation and an absence of secondary growth
metabolism
-
DcuS and CitA are involved in the regulation of carboxylate metabolism
physiological function
-
functional HK5 histidine kinase is required for H2O2 responses in stomatal guard cells
physiological function
P39453
the TorS histidine kinase activates the trimethylamine-N-oxide reductase pathway when sensing trimethylamine-N-oxide in the environment
physiological function
-
the TorS histidine kinase activates the trimethylamine-N-oxide reductase pathway when sensing trimethylamine-N-oxide in the environment
physiological function
Q66WP9
the NIK1 gene is involved in ambruticin susceptibility
physiological function
-
histidine kinase Ppr functions as a UV-red light sensor
physiological function
-
NikA is responsible for the responses to fungicides such as iprodione and fludioxonil, NikA functions as sensor upstream of the response regulators SskA and SrrA in response to fungicides
physiological function
-
histidine kinase Chk1p HK is part of a functionally similar but parallel pathway to the Sho1p-Cek1p pathway that confers resistance to the cell wall inhibitors Congo red and calcofluor white
physiological function
-
the VirA receiver domain is required for efficient vir gene expression, including the transcriptional induction of virG, VirA's receiver domain acts as a recruitment and/or alignment factor that increases the availability of VirG for phosphate transfer from VirA's kinase region to the VirG receiver domain
physiological function
-
CckA has a central role in establishing the cell cycle periodicity of CtrA activity by controlling both its phosphorylation and stability
physiological function
-
HP0244 is required for inner membrane assembly of UreA, UreB, and UreE and for urease activity
physiological function
-
the histidine kinase SphS is involved in transcriptional activation of the phosphate (Pi)-acquisition system
physiological function
-
histidine kinase KinA promotes the initiation of sporulation when nutrients are limiting
physiological function
-
the AgrC receptor histidine kinase detects its autoinducing peptide ligand and generates an intracellular signal resulting in secretion of virulence factors
physiological function
-
KdpD regulates the expression of potassium transporters in response to osmolarity
physiological function
-
FixL utilizes a heme bound to an N-terminal PAS domain to detect the amount of oxygen present in the cytoplasm through direct binding
physiological function
Penicillium marneffei
-
HHK1 regulates polar growth, sporulation, and cell wall composition
physiological function
-
cytokinin-independent1 and Arabidopsis histidine kinase2 and 3 regulate vascular tissue development in Arabidopsis shoots, the cytokinin-independent activity of CKI1 and cytokinin-induced histidine kinase2 and histidine kinase3 are important for vascular bundle formation in Arabidopsis
physiological function
-
the histidine kinase-related domain of phytochrome A controls the spectral sensitivity and the subcellular distribution of the photoreceptor
physiological function
Candida albicans REP36-1
-
histidine kinase Chk1p HK is part of a functionally similar but parallel pathway to the Sho1p-Cek1p pathway that confers resistance to the cell wall inhibitors Congo red and calcofluor white
-
physiological function
Emericella nidulans BPU1
-
NikA is responsible for the responses to fungicides such as iprodione and fludioxonil, NikA functions as sensor upstream of the response regulators SskA and SrrA in response to fungicides
-
physiological function
Vibrio parahaemolyticus EB101
-
the TorS histidine kinase activates the trimethylamine-N-oxide reductase pathway when sensing trimethylamine-N-oxide in the environment
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ArcA + ATP
?
show the reaction diagram
-
ArcB undergoes autophosphorylation at the expense of ATP and subsequently transphosphorylates its cognate response regulator ArcA through a His to Asp to His to Asp phosphorelay pathway
-
-
?
ArcA + ATP
?
show the reaction diagram
-
ArcB undergoes autophosphorylation at the expense of ATP and subsequently transphosphorylates its cognate response regulator ArcA through a His to Asp to His to Asp phosphorelay pathway
-
-
-
ArcA + ATP
?
show the reaction diagram
-
the arcB gene encodes a sensor-regulator protein for anaerobic repression of the arc modulon
-
-
-
ArcA + ATP
?
show the reaction diagram
-
the ArcB and ArcA proteins constitute a two-component signal transduction system that plays a broad role in transcriptional regulation. Under anoxic or environmentally reducing conditions, the sensor kinase ArcB is stimulated to autophosphorylate at the expense of ATP and subsequently transphosphorylates the response regulator ArcA
-
-
-
ArcA + ATP
?
show the reaction diagram
-
phosphoryl group transfer from phosphorylated ArcB to ArcA, signal transmission occurs solely by His-Asp-His-Asp phosphorelay
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q44007
the two-component regulatory system CzcS/CzcR is involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
kinase of the alternate pathway for phosphorylating the SpoOF protein
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
P0A4I6
enzyme is involved in early steps of competence regulation
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
photosynthesis gene expression in Rhodobacter sphaeroides is controlled in part by the two-component regulatory system composed of a membrane-bound sensor kinase PrrB and a response regulator PrrA
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
regB is part of a two-component system and encodes a sensor kinase involved in the global regulation of both anoxygenic light-dependent- and oxygenic light-independent CO2 fixation as well as anoxygenic photosystem biosynthesis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the tyrosine kinase DivL function in cell cycle and developmental regulation is mediated, at least in part, by the global response regulator CtrA, the enzyme is essential for cell viability and division
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q48768
enzyme is involved in signal transduction controlling chemotaxis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the essential two-component regulatory system yycF/yycG modulates expression of the ftsAZ operon in Bacillus subtilis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
enzyme is involved in chemotaxis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q53135
enzyme is involved in chemotaxis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q9RZA4
Deinococcus radiodurans bacteriophytochrome functions as a light-regulated histidine kinase, which helps protect the bacterium from visible light
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q3J6C1
PrrB is responsive to the removal of oxygen and functions through the response regulator PrrA. Together, prrB and prrA provide the major signal involved in synthesis of the specialized intracytoplasmic membrane, harboring components essential to the light reactions of photosynthesis. PrrB is a global regulator of photosynthesis gene expression
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q08408
regulation of the levels of OmpF and OmpC is normally controlled by a multicomponent signal-transducing regulatory pair of proteins, EnvZ and OmpR. The effect RprX and RprY have on OmpF expression is mediated at the level of transcription. Thus, RprX and RprY may be interfering with the normal regulation of OmpF by OmpR and EnvZ
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the two-component sensory transduction system chvG/chvI is required for virulence of Agrobacterium tumefaciens
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q06240
the two-component regulatory system VanS-VanR activates a promoter used for cotranscription of the vanH, vanA, and vanX resistance genes
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the two-component signal transduction system yycF/yycG is essential for growth of Bacillus subtilis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Enterococcus faecium BM4147
Q06240
the two-component regulatory system VanS-VanR activates a promoter used for cotranscription of the vanH, vanA, and vanX resistance genes
-
-
-
ATP + BctC
?
show the reaction diagram
-
-
-
-
?
ATP + CpdR-L-histidine
ADP + CpdR-N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + CtrA-L-histidine
ADP + CtrA-N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + DivK
?
show the reaction diagram
-
DivJ is the main kinase of DivK
-
-
?
ATP + FixJ
?
show the reaction diagram
-
-
-
-
?
ATP + histidine kinase EnvZ
ADP + histidine kinase EnvZ N-phospho-L-histidine
show the reaction diagram
-
autophosphorylation. Probing catalytically essential domain orientation in histidine kinase EnvZ by targeted disulfide crosslinking
-
-
?
ATP + histidine kinase Hik34
ADP + histidine kinase Hik34 N-phospho-L-histidine
show the reaction diagram
-
histidine kinase Hik34 might negatively regulate the expression of certain heat shock genes that might by related to thermotolerance in Synechocystis, autophosphorylation, in vitro at physiological temperatures, but not at elevated temperatures, such as 44C
-
-
?
ATP + PhoN protein L-histidine
ADP + PhoN protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
-
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
O34757
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
Q66WP9
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
AtBphP1 contains a typical two-component histidine kinase domain at its C-terminus whose activity is repressed after photoconversion to Pfr, AtBphP2 is repressed after photoconversion to Pr
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
histidine kinase BA1351, histidine kinase BA1356 is capable of inducing sporulation, histidine kinase BA1478, histidine kinase BA2291 acts as a phosphatase on the sporulation phosphorelay, histidine kinase BA2636, histidine kinase BA2644, histidine kinase BA3702, histidine kinase BA4223 is capable of inducing sporulation in Bacillus anthracis, histidine kinase BA5029
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
histidine kinase CikA resets the circadian clock
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
histidine kinases DivJ and PleC initiate signal transduction pathways that regulate an early cell division cycle step and the gain of motility later in the Caulobacter crescentus cell cycle
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals, in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals. The Hik16/Hik41 system responds only to NaCl
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
O48929
induced by dehydration and CaCl2. NTHK2 possesses Ser/Thr kinase activity in presence of Mn2+ and histidine kinase activity in presence of Ca2+
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
istidine kinases DivJ and PleC initiate signal transduction pathways that regulate an early cell division cycle step and the gain of motility later in the Caulobacter crescentus cell cycle
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
RodK may regulate multiple temporally separated events during fruiting body formation including stimulation of early developmental gene expression, inhibition of A-signal production and inhibition of the intercellular C-signal transduction pathway
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the circadian clock-associated histidine kinase SasA is necessary for rubustness of the circadian rhythm of gene expression and involved in clock output
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
Q9Z5F5
the gene-disrupted mutant is unable to produce normal mature fruiting bodies and produces fewer spores
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the three-component system of histidine kinases and response regulator, His16-Hik41-Rre17, acts as transducer of hyperosmotic stress
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the two-component histidine kinase alrO117 is involved in heterocyst development
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the two-component system of histidine kinase and response regulator, His10-Rre3, acts as transducer of hyperosmotic stress, the two-component system of histidine kinase and response regulator, His33-Rre31, acts as transducer of hyperosmotic stress, the two-component systes of histidine kinase and response regulator, His34-Rre1, acts as transducer of hyperosmotic stress
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
Synechococcus elongatus PCC7942
-
the circadian clock-associated histidine kinase SasA is necessary for rubustness of the circadian rhythm of gene expression and involved in clock output
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
Streptomyces reticuli Tue54
-
-
-
-
?
ATP + Spo0F protein L-histidine
ADP + Spo0F protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + VicR protein L-histidine
ADP + VicR protein N-phospho-L-histidine
show the reaction diagram
Streptococcus pneumoniae, Streptococcus pneumoniae (D39)
Q9S1J9
-
-
-
?
ATP + VirG-L-histidine
ADP + VirG-N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + YycF
ADP + phospho-YycF
show the reaction diagram
Q45614
the formation of the division septum is necessary for YycG phosphorylation of YycF, a response regulator/transcription factor
-
-
?
BvgA + ATP
?
show the reaction diagram
-
-
-
-
?
BvgA + ATP
?
show the reaction diagram
-
the phosphorylated, purified C-terminal domain alone is sufficient for phosphotransfer to BvgA
-
-
?
BvgA + ATP
?
show the reaction diagram
-
the cytoplasmic portion of BvgS ('BvgS)
-
-
?
BvgA + ATP
?
show the reaction diagram
-
one hybrid histidine kinase consisting of the BvgS transmitter and HPt domains and of the EvgS receiver domain BvgS-TO-EvgS-R is able to phosphorylate BvgA but not EvgA. In contrast, the hybrid protein consisting of the BvgS transmitter and the EvgS receiver and HPt domains BvgS-T-EvgS-RO is unable to phosphorylate BvgA but efficiently phosphorylates EvgA
-
-
?
CitB + ATP
?
show the reaction diagram
-
a fusion protein MalE-CitAC is composed of the maltose-binding protein and the CitA kinase domain shows constitutive autokinase activity and transfers the gamma-phosphate group of ATP to its cognate response regulator CitB
-
-
?
CtrA + ATP
?
show the reaction diagram
Q9RQQ9
-
-
-
?
DcuR + ATP
?
show the reaction diagram
-
the phosphoryl group of DcuS is rapidly transferred to the response regulator DcuR. Upon phosphorylation, DcuR binds specifically to dcuB promoter DNA
-
-
?
EvgA + ATP
?
show the reaction diagram
-
one hybrid histidine kinase consisting of the BvgS transmitter and HPt domains and of the EvgS receiver domain BvgS-TO-EvgS-R is able to phosphorylate BvgA but not EvgA. In contrast, the hybrid protein consisting of the BvgS transmitter and the EvgS receiver and HPt domains BvgS-T-EvgS-RO is unable to phosphorylate BvgA but efficiently phosphorylates EvgA
-
-
?
GTP + protein-L-histidine
GDP + protein N-phospho-L-histidine
show the reaction diagram
-, Q81QX4
the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview
-
-
r
GTP + protein-L-histidine
GDP + protein N-phospho-L-histidine
show the reaction diagram
-, Q81QX4
BA2291 is absolutely specific for guanine nucleotides in both the forward and reverse reactions, nucleotide specificity of BA2291, overview
-
-
r
GTP + protein-L-histidine
GDP + protein N-phospho-L-histidine
show the reaction diagram
Bacillus anthracis 34F2DELTA118
Q81QX4
the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview, BA2291 is absolutely specific for guanine nucleotides in both the forward and reverse reactions, nucleotide specificity of BA2291, overview
-
-
r
GTP + Spo0F L-histidine
GDP + Spo0F N-phospho-L-histidine
show the reaction diagram
-, Q81QX4
a protein purified from Bacillus subtilis
-
-
r
protein + ATP
?
show the reaction diagram
-
the cytoplasmic portion of BvgS autophosphorylates with the gamma-phosphate from [gamma-32P]ATP
-
-
?
protein + ATP
?
show the reaction diagram
-
autophosphorylation
-
-
?
protein + ATP
?
show the reaction diagram
O34206
autophosphorylation
-
-
?
protein + ATP
?
show the reaction diagram
-
autophosphorylation
-
-
?
protein + ATP
?
show the reaction diagram
-
autophosphorylation
-
-
-
protein + ATP
?
show the reaction diagram
-
autophosphorylation
-
-
?
protein + ATP
?
show the reaction diagram
-
autophosphorylation
-
-
?
protein + ATP
?
show the reaction diagram
-
autophosphorylation
-
-
?
protein + ATP
?
show the reaction diagram
P39664
autophosphorylation
-
-
?
protein + ATP
?
show the reaction diagram
-
a model of the mechanism of FrzE phosphorylation: autophosphorylation initially occurs at a conserved His residue within the "CheA" domain and then, via an intramolecular transphosphorylation, is transferred to a conserved aspartate residue within the "CheY" domain
-
-
?
protein + ATP
?
show the reaction diagram
-
DivL protein is homologous to the ubiquitous bacterial histidine protein kinases, it differs from previously studied members of this protein kinase family in that it contains a tyrosine residue Tyr550 in the conserved H-box instead of a histidine residue, which is the expected site of autophosphorylation. DivL is autophosphorylated on Tyr-550 in vitro, and this tyrosine residue is essential for cell viability and regulation of the cell division cycle
-
-
?
protein + ATP
?
show the reaction diagram
-
H243 is a site of autophosphorylation as well as transphosphorylation to the conserved D55 residue of response regulator OmpR
-
-
?
Rcp1 + ATP
?
show the reaction diagram
-
Cph1 is a light-regulated histidine kinase that mediates red, far-red reversible phosphorylation of the a small response regulator Rcp1
-
-
-, ?
regulator protein OmpR + ATP
?
show the reaction diagram
P0AEJ4
-
-
-
?
regulator protein OmpR + ATP
?
show the reaction diagram
-
H243 is the a site of autophosphorylation as well as transphosphorylation to the conserved D55 residue of response regulator OmpR
-
-
?
TorR + ATP
?
show the reaction diagram
-
TorS is a sensor that contains three phosphorylation sites and transphosphorylates TorR via a four-step phosphorelay, His443 to Asp723 to His850 to Asp(TorR). TorS can dephosphorylate phospho-TorR when trimethylamine N-oxide is removed. Dephosphorylation probably occurs by a reverse phosphorelay, Asp(TorR) to His850 to Asp723
-
-
?
GTP + Spo0F L-histidine
GDP + Spo0F N-phospho-L-histidine
show the reaction diagram
Bacillus anthracis 34F2DELTA118
Q81QX4
a protein purified from Bacillus subtilis
-
-
r
additional information
?
-
-
mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regulatory proteins. Spo0F protein is a much better phosphoreceptor for this kinase than Spo0A protein in vitro
-
-
-
additional information
?
-
-
BarA/UvrY system activated biofilm formation. UvrY resides downstream from csrA in a signaling pathway for csrB and CsrA stimulates UvrY-dependent activation of csrB expression by BarA-dependent and BarA-independent mechanisms
-
-
-
additional information
?
-
-
DNA sequences of plnB reveals that the product closely resembles members of bacterial two-component signal transduction systems. The finding that plnABCD are transcribed from a common promoter suggests that the biological role played by the bacteriocin is somehow related to the regulatory function of the two-component system located on the same operon
-
-
-
additional information
?
-
-
ompR-envZ is a two component regulatory system that plays an important role in the regulation of Vi polysaccharide synthesis in Salmonella typhi. One of the environmental signals for this regulation may be osmolarity
-
-
-
additional information
?
-
P0AEC7
E. coli BarA-UvrY two-component system is required for efficient switching between glycolytic and gluconeogenic carbon sources
-
-
-
additional information
?
-
-
purified BarA protein is able to autophosphorylate when incubated with [gamma-(32)P]ATP but not with [alpha-(32)P]ATP or [gamma-(32)P]GTP. Phosphorylated BarA, in turn, acts as an efficient phosphoryl group donor to UvrY. BarA and UvrY constitute a new two-component system for gene regulation in Escherichia coli
-
-
-
additional information
?
-
-
enzyme is involved in adaptive responses in E. coli
-
-
-
additional information
?
-
-
the VanR B-VanS B two-component regulatory system activates a promoter located immediately downstream from the vanS B gene
-
-
-
additional information
?
-
-
CpxA functions as a transmembrane sensory protein
-
-
-
additional information
?
-
-
EnvZ modulates expression of the ompF and ompC genes through phosphotransfer signal transduction in Escherichia coli
-
-
-
additional information
?
-
-
enzyme controls the osmoregulated biosynthesis of the porin proteins OmpF and OmpC
-
-
-
additional information
?
-
-
the enzyme plays a central role in osmoregulation, a cellular adaptation process involving the His-Asp phosphorelay signal transduction system. Dimerization of the transmembrane protein is essential for its autophosphorylation and phosphorelay signal transduction functions
-
-
-
additional information
?
-
-, O82436
Cm-ETR1 mRNA is very high in the seeds and placenta. Marked increase of Cm-ETR1 mRNA parallels climacteric ethylene production. Cm-ETR1 plays a specific role not only in ripening but also in the early development of melon fruit
-
-
-
additional information
?
-
-
the enzyme plays an important role in coupling signals received from membrane-bound receptors to changes in the swimming behavior of the cells in order to respond appropriately to environmental signals
-
-
-
additional information
?
-
-
a complex of the proteins CheA (CheAL and CheAS) and CheW constitutes a functional unit that responds to the signaling state of the chemoreceptors. The autophosphorylation rate of CheAL is much greater when CheAL and CheAS are complexed with CheW. Moreover, the presence of mutant chemoreceptors that cause cells to tumble increases this rate. At wild-type levels of expression, the isolated CheAL/CheAS/CheW complex accounts for about 10% of the total number of CheAL, CheAS, and CheW molecules and exists in a 1:1:1 stoichiometry. This complex is also required for CheAL/CheAS and CheW binding to the phosphorylation substrate, CheY
-
-
-
additional information
?
-
P07167, P07168
it is proposed that VirA acts as an environmental sensor of plant-derived inducer molecules and transmits this information to the level of vir gene expression
-
-
-
additional information
?
-
O31396
the two-component regulatory system irlR-irlS is involved in invasion of eukaryotic cells and heavy-metal resistance in Burkholderia pseudomallei
-
-
-
additional information
?
-
O32193
two-component regulatory system CssR-CssS, is required for the cell to survive the severe secretion stress caused by a combination of high-level production of the alpha-amylase AmyQ and reduced levels of the extracytoplasmic folding factor PrsA. The Css system is required to degrade misfolded exported proteins at the membrane-cell wall interface. CssS represents the first identified sensor for extracytoplasmic protein misfolding in a Gram-positive eubacterium
-
-
-
additional information
?
-
-
the CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis
-
-
-
additional information
?
-
-
the protein is involved in osmoregulation of OmpF and OmpC. EnvZ is considered to be an osmosensor which transmits signals across the membrane to OmpR, a transcriptional activator for ompF and ompC
-
-
-
additional information
?
-
-
UhpB and perhaps UhpC play both positive and negative roles in the control of uhpT transcription
-
-
-
additional information
?
-
P27668
UhpB and perhaps UhpC play both positive and negative roles in the control of uhpT transcription
-
-
-
additional information
?
-
-
regulation of nitrogen fixation genes in Rhizobium meliloti is mediated by two proteins, FixL and FixJ, in response to oxygen availability, oxygen sensor
-
-
-
additional information
?
-
-
the gene regulates transcription of the nifHDK operon and so limits the expression of nitrogen fixation activity to periods of low environmental concentrations of both oxygen and fixed nitrogen
-
-
-
additional information
?
-
-
FixL senses an environmental signal and transduces it to FixJ, a transcriptional activator of nif and fix genes
-
-
-
additional information
?
-
P10047
required for the activation of the C4-dicarboxylate transport structural gene dctA in free-living Rhizobium leguminosarum
-
-
-
additional information
?
-
P09384
during bacterial chemotaxis, the binding of stimulatory ligands to chemoreceptors at the cell periphery leads to a response at the flagellar motor. Three proteins appear to be required for receptor-mediated control of swimming behavior, the products of the cheA, cheW, and cheY genes
-
-
-
additional information
?
-
P08982
enzyme is required for the proper expression of the outer membrane proteins OmpC and OmpF
-
-
-
additional information
?
-
-
enzyme has an enhancing effect on the transcription of phoA, primary function may not be connected to the phosphate regulon
-
-
-
additional information
?
-
P10799
membrane-bound sensor of plant signal molecules
-
-
-
additional information
?
-
P18769
FrzE is a second messenger that relays information between the signaling protein FrzCD and the gliding motor
-
-
-
additional information
?
-
-
mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regulatory proteins
-
-
-
additional information
?
-
P14376
RcsC is the sensor components of the two-component regulatory system which regulates expression of the slime polysaccharide colanic acid. rcs system is essential for expression of high levels of the group I capsular polysaccharide in lon+ E. coli K30
-
-
-
additional information
?
-
-
the two-component regulatory system phoP/phoQ controls Salmonella typhimurium virulence
-
-
-
additional information
?
-
P14376
colanic acid capsule synthesis in Escherichia coli K-12 is regulated by RcsB and RcsC. RcsC acts as the sensor and RcsB acts as the receiver or effector to stimulate capsule synthesis from cps genes
-
-
-
additional information
?
-
-
bvgS and bvgA control the expression of the virulence-associated genes in Bordetella species by a system similar to the two-component systems used by a variety of bacterial species to respond to environmental stimuli
-
-
-
additional information
?
-
P14147
the PhoP-PhoQ system exerts a master regulatory function for preventing bacterial overgrowth within fibroblasts
-
-
-
additional information
?
-
P14377
the HydH/G system senses high periplasmic Zn2+ and Pb2+ concentrations and contributes to metal tolerance by activating the expression of zraP
-
-
-
additional information
?
-
Q9APE0
the HydH/G system senses high periplasmic Zn2+ and Pb2+ concentrations and contributes to metal tolerance by activating the expression of zraP
-
-
-
additional information
?
-
P15939
NodV and NodW proteins are members of the family of two-component regulatory systems, NodV responds to an environmental stimulus and, after signal transduction, NodW may be required to positively regulate the transcription of one or several unknown genes involved in the nodulation process
-
-
-
additional information
?
-
P20169
the enzyme is involved in chemical sensing
-
-
-
additional information
?
-
-
genes dctB and dctD form a two-component system which responds to the presence of C4-dicarboxylates to regulate expression of a transport protein encoded by dctA
-
-
-
additional information
?
-
-
narL and narX mediate nitrate induction of nitrate reductase synthesis and nitrate repression of fumarate reductase synthesis
-
-
-
additional information
?
-
-
in free-living cells, the regulatory dctBD genes are absolutely required for the expression of the dctA gene
-
-
-
additional information
?
-
-
dctB-encoded protein includes a putative periplasmic N-terminal domain that senses the presence of dicarboxylates and a C-terminal cytoplasmic domain that activates the dctD-encoded protein
-
-
-
additional information
?
-
-
enzyme is involved in signal transduction
-
-
-
additional information
?
-
-
the enzyme is a regulator of chemotaxis
-
-
-
additional information
?
-
P33113
enzyme is responsible for regulation of subtilin biosynthesis
-
-
-
additional information
?
-
-
enzyme is responsible for regulation of subtilin biosynthesis
-
-
-
additional information
?
-
-
enzyme is involved in the regulation of expression of phosphoglycerate transport in Salmonella typhimurium. pgtB and pgtC genes are involved in the induction of the pgtP expression by modulating derepressor activity
-
-
-
additional information
?
-
P29905
moxY is part of the two-component regulatory system controlling methanol dehydrogenase synthesis
-
-
-
additional information
?
-
-
either of two functionally redundant sensor proteins, NarX and NarQ, is sufficient for nitrate regulation in Escherichia coli K-12. NarQ and NarX may have subtle functional differences
-
-
-
additional information
?
-
-
activation role for ResD, and to a lesser extent ResE, in global regulation of aerobic and anaerobic respiration in Bacillus subtilis
-
-
-
additional information
?
-
P37433
PgtB and PgtC polypeptides modulate PgtA activity
-
-
-
additional information
?
-
-
narQ is a nitrate sensor for nitrate-dependent gene regulation in Escherichia coli
-
-
-
additional information
?
-
-
the enzyme is a biological oxygen sensors that restricts the expression of specific genes to hypoxic conditions
-
-
-
additional information
?
-
P21865
the two-component sensor-effector system KdpD /KdpE controls expression of the kdpABC operon
-
-
-
additional information
?
-
-
the two-component sensor-effector system KdpD /KdpE controls expression of the kdpABC operon
-
-
-
additional information
?
-
-
FixL and FixJ proteins are members of the two-component sensor/regulator family
-
-
-
additional information
?
-
P33639
PilS/PilR is a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa. PilS is a sensor protein which when stimulated by the appropriate environmental signals activates PilR through kinase activity. PilR then activates transcription of pilA, probably by interacting with RNA polymerase containing RpoN
-
-
-
additional information
?
-
-
the TorS/TorR two-component system induces the expression of the tor structural operon encoding the trimethylamine N-oxide reductase respiratory system in response to substrate availability. TorS belongs to a sensor subfamily that includes a classical transmitter domain, a receiver, and a C-terminal alternative transmitter domain
-
-
-
additional information
?
-
P49333
ETR1 acts as an ethylene receptor
-
-
-
additional information
?
-
-
ETR1 acts as an ethylene receptor
-
-
-
additional information
?
-
-
TorS is a sensor that contains three phosphorylation sites and transphosphorylates TorR via a four-step phosphorelation, His443--Asp723--His850--Asp(TorR)
-
-
-
additional information
?
-
P40719, Q8X524
QseBC is a two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli strains EHEC and K-12
-
-
-
additional information
?
-
-
TorS mediates the induction of the tor structural genes in response to trimethylamine N-oxide
-
-
-
additional information
?
-
-
enzyme is involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris
-
-
-
additional information
?
-
P45608
enzyme is involved in regulation of the phosphate regulon
-
-
-
additional information
?
-
P45609
enzyme is involved in regulation of the phosphate regulon
-
-
-
additional information
?
-
-
the two-component regulatory system DcuSR of Escherichia coli controls the expression of genes of C4-dicarboxylate metabolism in response to extracellular C4-dicarboxylates such as fumarate or succinate. The phosphoryl group of DcuS is rapidly transferred to the response regulator DcuR. Upon phosphorylation, DcuR binds specifically to dcuB promoter DNA
-
-
-
additional information
?
-
P52687
citrate, Na+, and oxygen exert their regulatory effects via the CitA/CitB system. In the presence of these signals, the citAB gene products induce their own synthesis. The positive autoregulation occurrs via co-transcription of citAB with citS and oadGAB
-
-
-
additional information
?
-
-
the genes encoding the anaerobic fumarate respiratory system are transcriptionally regulated by C4-dicarboxylates. The regulation is effected by a two-component regulatory system, DcuSR, consisting of a sensory histidine kinase DcuS and a response regulator DcuR
-
-
-
additional information
?
-
-
periplasmic loop of DcuS serves as a C4-dicarboxylate sensor. The cytosolic region of DcuS contains two domains: a central PAS domain possibly acting as a second sensory domain and a C-terminal transmitter domain
-
-
-
additional information
?
-
-
the two-component system regulates an osmosensing MAP kinase cascade
-
-
-
additional information
?
-
P77485
expression of cusC is induced by high concentrations of copper ions, the cusRS two-component signal transduction system is required for copper-induced expression of pcoE, a plasmid-borne gene from the E. coli copper resistance operon pco. The genes cusRS are also required for the copper-dependent expression of at least one chromosomal gene, designated cusC, which is allelic to the recently identified virulence gene ibeB in E. coli K1. The cus locus may comprise a copper ion efflux system
-
-
-
additional information
?
-
Q06067
the antizyme is a bifunctional protein serving as both an inhibitor of polyamine biosynthesis as well as a transcriptional regulator of an as yet unknown set of genes
-
-
-
additional information
?
-
-
the enzyme is involved in chemotaxis
-
-
-
additional information
?
-
Q02541
two-component regulatory system CopR/CopS is required for copper-inducible expression of the copper resistance operon
-
-
-
additional information
?
-
Q04850
the two-component regulatory system, NtrY/NtrX is involved in nitrogen fixation and metabolism. NtrY is likely to represent the transmembrane sensor protein element in a two-component regulatory system
-
-
-
additional information
?
-
Q04943
the two-component regulatory system afsQ1/afsQ2 is involved in secondary metabolism
-
-
-
additional information
?
-
P77510
the two-component systemDpiA/DpiB is involved in regulation of plasmid inheritance
-
-
-
additional information
?
-
P0A4I7
the cutR-cutS operon regulates copper metabolism in Streptomyces
-
-
-
additional information
?
-
P52687
rhe periplasmic domain of the histidine autokinase CitA functions as a highly specific citrate receptor
-
-
-
additional information
?
-
-
the ExoS-ChvI two-component regulatory system regulates succinoglycan production. ChvG is the sensor protein of the ChvG-ChvI two-component regulatory system
-
-
-
additional information
?
-
P0C5S6
the enzyme is involved in regulation of density-dependent expression of luminescence in Vibrio harveyi
-
-
-
additional information
?
-
P54302
the enzyme is involved in regulation of density-dependent expression of luminescence in Vibrio harveyi
-
-
-
additional information
?
-
-
the two-component regulatory system PfeR/PfeS is involved in the expression of the ferric enterobactin receptor PfeA
-
-
-
additional information
?
-
-
deletion of PilS results in a non-pilated phenotype
-
-
-
additional information
?
-
Q9X180
autophosphorylation activity
-
-
-
additional information
?
-
-
autophosphorylation of MtrB-Strep in proteoliposomes in the presence of ATP
-
-
-
additional information
?
-
-
full-length His-RaxH shows autophosphorylation activities
-
-
-
additional information
?
-
-
neither HK1 nor HK2 is able to autophosphorylate itself, HK1 is an ATP binding protein, acts as a functional kinase and phosphorylates HK2 by interacting with it, transfer of a phosphoryl group from HK2 to the response regulator TcrA
-
-
-
additional information
?
-
-
no autophosphorylation of wild-type, is caused by the rate of dephosphorylation being higher than the rate of autophosphorylation
-
-
-
additional information
?
-
-
upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation
-
-
-
additional information
?
-
P0AEC8
a chemical or other stimulus is detected by the periplasmic sensor domain of a transmembrane histidine kinase sensor, which in turn relays a signal through a phosphotransfer cascade to the cognate cytoplasmic response regulator. Such systems lead ultimately to changes in gene expression or cell motility. Mechanisms of ligand binding and signal transduction through the cell membrane, overview
-
-
-
additional information
?
-
-, Q09JB2, Q09JB3, Q09JB4, Q09JB6, Q09JB7, Q09JB8, Q09JB9
AlHK1p is important for, but not the sole factor responsible for Alternaria longipes resistance to dicarboximide fungicides, e.g. dimethachlon, molecular basis of the fungicide resistance, overview
-
-
-
additional information
?
-
-
DcuS is the C4-dicarboxylate sensor of Escherichia coli catalyzing transmembrane sensing
-
-
-
additional information
?
-
-, Q81QX4
genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay
-
-
-
additional information
?
-
-
histidine kinases are part of two-component signal transduction systems that act to integrate environmental stimuli into a cellular response via a phosphotransfer relay mechanism, e.g. involved in stomatal guard cell response to H2O2, regulation of the physiological function, overview
-
-
-
additional information
?
-
Q95434
Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview
-
-
-
additional information
?
-
-
role of the C-terminal domains in the photocycle of a light sensor histidine kinase Ppr having a photoactive yellow protein, PYP, domain as the photosensor domain, photocycles of the PYP domain of Ppr and of the full-length Ppr. The, overview
-
-
-
additional information
?
-
Q9HWR3
signal transduction mechanism in the bacteriophytochrome, overview
-
-
-
additional information
?
-
Q88PG3
significant number of genes are influenced directly or indirectly by the RoxSR two-component system. 173 genes are found showing reduced expression in EU58 (RoxSR mutant strain) in comparison to the wild type, whereas 84 genes are upregulated in the mutant
-
-
-
additional information
?
-
Q25026
the enzyme HP0244 is not only required to regulate flagellar gene expression via its cognate response regulator, HP0703, but also to generate a response to declining medium pH. Although not required for survival at pH 4.5, HP0244 is required for survival at pH 2.5, overview
-
-
-
additional information
?
-
P16497
the N-terminal Per-ARNT-Sim domain plays a critical role in the catalytic activity of this enzyme, a significant decrease occurs of the autophosphorylation rate of a KinA protein lacking this domain
-
-
-
additional information
?
-
-
the SenS/SenR system of Streptomyces reticuli regulates the expression of the redox regulator FurS, the catalase-peroxidase CpeB and the heme-binding protein HbpS. SenS/SenR also participates in sensing redox changes, mediated by HbpS. The heme-binding protein HbpS regulates the activity of the Streptomyces reticuli iron-sensing histidine kinase SenS in a redox-dependent manner, presence of SenS/SenR leads to the synthesis of extracellular redox active proteins, overview
-
-
-
additional information
?
-
Q45614
The sensor histidine kinase YycG acts in a two-component system with response regulator/transcription factor YycF in Bacillus subtilis controling the synthesis of autolysins and autolysin inhibitors, that function in cell wall remodelling and cell separation, YycG sensor histidine kinase is a component of and perceives infirmation at the division septum in growing cells constituting a positive feedback loop, that serves to co-ordinate cell division with cell wall homeostasis, overview
-
-
-
additional information
?
-
-
the sensory histidine kinase acts in the two-component system with the response regulator as RR-HK17, EF1633-EF1632, in the regulation of ethanolamine utilization, which can be the sole carbon source for the organism, mechanism, overview
-
-
-
additional information
?
-
Q88PG3
The two-component system is implicated in redox signaling and cytochrome oxidase activity, in expression of the cell density-dependent gene ddcA and in bacterial colonization of plant surfaces. The RoxS/RoxR regulon includes genes involved in sugar and amino acid metabolism and the sulfur starvation response and elements of the respiratory chain (a cbb3 cytochrome oxidase, Fe-S clusters, and cytochrome c-related proteins) or genes participating in the maintenance of the redox balance. The RoxS/RoxR system controls a broad set of functions that have an influence on energy metabolism, such as formaldehyde and formate dehydrogenases.
-
-
-
additional information
?
-
-, Q81QX4
BA2291 performs GTP-dependent autophosphorylation
-
-
-
additional information
?
-
-, Q09JB2, Q09JB3, Q09JB4, Q09JB6, Q09JB7, Q09JB8, Q09JB9
histidine kinase domain and response regulator domain form a two-component system
-
-
-
additional information
?
-
P16497
KinA performs autophosphorylation
-
-
-
additional information
?
-
-
SenS performs autophosphorylation. HbpS/SenS interaction analysis, overview
-
-
-
additional information
?
-
Q9HWR3
structure-function relationship: the bacteriophytochrome possesses a histidine kinase domain and undergoes conformational changes during photoconversion, local structural changes originating in the photosensory domain modulate interactions between long, crossdomain signaling helices at the dimer interface and are transmitted to the spatially distant effector domain, thereby regulating its histidine kinase activity, overview
-
-
-
additional information
?
-
-
the enzyme performs regulatory autophosphorylation
-
-
-
additional information
?
-
-
Cek1p phosphorylation via Sho1p does not require histidine kinase Chk1p
-
-
-
additional information
?
-
-
FrzE autophosphorylates the kinase domain at His-49, and phosphoryl groups are transferred to aspartate residues (Asp-52 and Asp-220) in the two receiver domains of FrzA. The FrzE receiver domain inhibits autophosphorylation of the FrzE kinase domain
-
-
-
additional information
?
-
Escherichia coli O9:K30:H12
P14376
RcsC is the sensor components of the two-component regulatory system which regulates expression of the slime polysaccharide colanic acid. rcs system is essential for expression of high levels of the group I capsular polysaccharide in lon+ E. coli K30
-
-
-
additional information
?
-
Pseudomonas syringae DC3000
-
upon illumination in the presence of ATP, the enzyme undergoes autophosphorylation
-
-
-
additional information
?
-
Agrobacterium tumefaciens 15955
P10799
membrane-bound sensor of plant signal molecules
-
-
-
additional information
?
-
Lactobacillus plantarum C11
-
DNA sequences of plnB reveals that the product closely resembles members of bacterial two-component signal transduction systems. The finding that plnABCD are transcribed from a common promoter suggests that the biological role played by the bacteriocin is somehow related to the regulatory function of the two-component system located on the same operon
-
-
-
additional information
?
-
Xanthomonas oryzae pv. oryzae PXO99
-
full-length His-RaxH shows autophosphorylation activities
-
-
-
additional information
?
-
Streptomyces reticuli Tue54
-
the SenS/SenR system of Streptomyces reticuli regulates the expression of the redox regulator FurS, the catalase-peroxidase CpeB and the heme-binding protein HbpS. SenS/SenR also participates in sensing redox changes, mediated by HbpS. The heme-binding protein HbpS regulates the activity of the Streptomyces reticuli iron-sensing histidine kinase SenS in a redox-dependent manner, presence of SenS/SenR leads to the synthesis of extracellular redox active proteins, overview, SenS performs autophosphorylation. HbpS/SenS interaction analysis, overview
-
-
-
additional information
?
-
Streptomyces reticuli Tue54
-
the SenS/SenR system of Streptomyces reticuli regulates the expression of the redox regulator FurS, the catalase-peroxidase CpeB and the heme-binding protein HbpS. SenS/SenR also participates in sensing redox changes, mediated by HbpS. The heme-binding protein HbpS regulates the activity of the Streptomyces reticuli iron-sensing histidine kinase SenS in a redox-dependent manner, presence of SenS/SenR leads to the synthesis of extracellular redox active proteins, overview, SenS performs autophosphorylation. HbpS/SenS interaction analysis, overview
-
-
-
additional information
?
-
Bacillus anthracis 34F2DELTA118
Q81QX4
genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay, BA2291 performs GTP-dependent autophosphorylation
-
-
-
additional information
?
-
Bacillus subtilis 1A40
P16497
the N-terminal Per-ARNT-Sim domain plays a critical role in the catalytic activity of this enzyme, a significant decrease occurs of the autophosphorylation rate of a KinA protein lacking this domain, KinA performs autophosphorylation
-
-
-
additional information
?
-
Candida albicans REP36-1
-
Cek1p phosphorylation via Sho1p does not require histidine kinase Chk1p
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ArcA + ATP
?
show the reaction diagram
-
ArcB undergoes autophosphorylation at the expense of ATP and subsequently transphosphorylates its cognate response regulator ArcA through a His to Asp to His to Asp phosphorelay pathway
-
-
-
ArcA + ATP
?
show the reaction diagram
-
the arcB gene encodes a sensor-regulator protein for anaerobic repression of the arc modulon
-
-
-
ArcA + ATP
?
show the reaction diagram
-
the ArcB and ArcA proteins constitute a two-component signal transduction system that plays a broad role in transcriptional regulation. Under anoxic or environmentally reducing conditions, the sensor kinase ArcB is stimulated to autophosphorylate at the expense of ATP and subsequently transphosphorylates the response regulator ArcA
-
-
-
ArcA + ATP
?
show the reaction diagram
-
phosphoryl group transfer from phosphorylated ArcB to ArcA, signal transmission occurs solely by His-Asp-His-Asp phosphorelay
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q44007
the two-component regulatory system CzcS/CzcR is involved in transcriptional control of heavy-metal homoeostasis in Alcaligenes eutrophus
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
kinase of the alternate pathway for phosphorylating the SpoOF protein
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
P0A4I6
enzyme is involved in early steps of competence regulation
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
photosynthesis gene expression in Rhodobacter sphaeroides is controlled in part by the two-component regulatory system composed of a membrane-bound sensor kinase PrrB and a response regulator PrrA
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
regB is part of a two-component system and encodes a sensor kinase involved in the global regulation of both anoxygenic light-dependent- and oxygenic light-independent CO2 fixation as well as anoxygenic photosystem biosynthesis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the tyrosine kinase DivL function in cell cycle and developmental regulation is mediated, at least in part, by the global response regulator CtrA, the enzyme is essential for cell viability and division
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q48768
enzyme is involved in signal transduction controlling chemotaxis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the essential two-component regulatory system yycF/yycG modulates expression of the ftsAZ operon in Bacillus subtilis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
enzyme is involved in chemotaxis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q53135
enzyme is involved in chemotaxis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q9RZA4
Deinococcus radiodurans bacteriophytochrome functions as a light-regulated histidine kinase, which helps protect the bacterium from visible light
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q3J6C1
PrrB is responsive to the removal of oxygen and functions through the response regulator PrrA. Together, prrB and prrA provide the major signal involved in synthesis of the specialized intracytoplasmic membrane, harboring components essential to the light reactions of photosynthesis. PrrB is a global regulator of photosynthesis gene expression
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q08408
regulation of the levels of OmpF and OmpC is normally controlled by a multicomponent signal-transducing regulatory pair of proteins, EnvZ and OmpR. The effect RprX and RprY have on OmpF expression is mediated at the level of transcription. Thus, RprX and RprY may be interfering with the normal regulation of OmpF by OmpR and EnvZ
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the two-component sensory transduction system chvG/chvI is required for virulence of Agrobacterium tumefaciens
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Q06240
the two-component regulatory system VanS-VanR activates a promoter used for cotranscription of the vanH, vanA, and vanX resistance genes
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
-
the two-component signal transduction system yycF/yycG is essential for growth of Bacillus subtilis
-
-
-
ATP + a protein
ADP + a phosphoprotein
show the reaction diagram
Enterococcus faecium BM4147
Q06240
the two-component regulatory system VanS-VanR activates a promoter used for cotranscription of the vanH, vanA, and vanX resistance genes
-
-
-
ATP + histidine kinase Hik34
ADP + histidine kinase Hik34 N-phospho-L-histidine
show the reaction diagram
-
histidine kinase Hik34 might negatively regulate the expression of certain heat shock genes that might by related to thermotolerance in Synechocystis
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
O34757
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
Q66WP9
-
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
AtBphP1 contains a typical two-component histidine kinase domain at its C-terminus whose activity is repressed after photoconversion to Pfr, AtBphP2 is repressed after photoconversion to Pr
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
canonical histidine kinase activity of the transmitter domain of the ETR1 ethylene receptor from Arabidopsis is not required for signal transmission
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
histidine kinase BA1351, histidine kinase BA1356 is capable of inducing sporulation, histidine kinase BA1478, histidine kinase BA2291 acts as a phosphatase on the sporulation phosphorelay, histidine kinase BA2636, histidine kinase BA2644, histidine kinase BA3702, histidine kinase BA4223 is capable of inducing sporulation in Bacillus anthracis, histidine kinase BA5029
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
histidine kinase CikA resets the circadian clock
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
histidine kinases DivJ and PleC initiate signal transduction pathways that regulate an early cell division cycle step and the gain of motility later in the Caulobacter crescentus cell cycle
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals, in Synechocystis sp. PCC 6803 four histidine kinases, Hik16, Hik33, Hik34, and Hik41, perceive and transduce salt signals. The Hik16/Hik41 system responds only to NaCl
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
O48929
induced by dehydration and CaCl2. NTHK2 possesses Ser/Thr kinase activity in presence of Mn2+ and histidine kinase activity in presence of Ca2+
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
istidine kinases DivJ and PleC initiate signal transduction pathways that regulate an early cell division cycle step and the gain of motility later in the Caulobacter crescentus cell cycle
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
RodK may regulate multiple temporally separated events during fruiting body formation including stimulation of early developmental gene expression, inhibition of A-signal production and inhibition of the intercellular C-signal transduction pathway
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the circadian clock-associated histidine kinase SasA is necessary for rubustness of the circadian rhythm of gene expression and involved in clock output
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
Q9Z5F5
the gene-disrupted mutant is unable to produce normal mature fruiting bodies and produces fewer spores
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the three-component system of histidine kinases and response regulator, His16-Hik41-Rre17, acts as transducer of hyperosmotic stress
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the two-component histidine kinase alrO117 is involved in heterocyst development
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
-
the two-component system of histidine kinase and response regulator, His10-Rre3, acts as transducer of hyperosmotic stress, the two-component system of histidine kinase and response regulator, His33-Rre31, acts as transducer of hyperosmotic stress, the two-component systes of histidine kinase and response regulator, His34-Rre1, acts as transducer of hyperosmotic stress
-
-
?
ATP + protein L-histidine
ADP + protein N-phospho-L-histidine
show the reaction diagram
Synechococcus elongatus PCC7942
-
the circadian clock-associated histidine kinase SasA is necessary for rubustness of the circadian rhythm of gene expression and involved in clock output
-
-
?
ATP + YycF
ADP + phospho-YycF
show the reaction diagram
Q45614
the formation of the division septum is necessary for YycG phosphorylation of YycF
-
-
?
GTP + protein-L-histidine
GDP + protein N-phospho-L-histidine
show the reaction diagram
-, Q81QX4
the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview
-
-
r
GTP + protein-L-histidine
GDP + protein N-phospho-L-histidine
show the reaction diagram
Bacillus anthracis 34F2DELTA118
Q81QX4
the enzyme is involved the transition between the vegetative cycle and sporulation, multiple sensor histidine kinases are induced to autophosphorylate in response to sporulation specific signals in the phosphorelay, the BA2291 protein may act as a phosphatase on the sporulation phosphorelay when present at elevated levels, overview
-
-
r
GTP + Spo0F L-histidine
GDP + Spo0F N-phospho-L-histidine
show the reaction diagram
-, Q81QX4
a protein purified from Bacillus subtilis
-
-
r
Rcp1 + ATP
?
show the reaction diagram
-
Cph1 is a light-regulated histidine kinase that mediates red, far-red reversible phosphorylation of the a small response regulator Rcp1
-
-
-
GTP + Spo0F L-histidine
GDP + Spo0F N-phospho-L-histidine
show the reaction diagram
Bacillus anthracis 34F2DELTA118
Q81QX4
a protein purified from Bacillus subtilis
-
-
r
additional information
?
-
-
BarA/UvrY system activated biofilm formation. UvrY resides downstream from csrA in a signaling pathway for csrB and CsrA stimulates UvrY-dependent activation of csrB expression by BarA-dependent and BarA-independent mechanisms
-
-
-
additional information
?
-
-
DNA sequences of plnB reveals that the product closely resembles members of bacterial two-component signal transduction systems. The finding that plnABCD are transcribed from a common promoter suggests that the biological role played by the bacteriocin is somehow related to the regulatory function of the two-component system located on the same operon
-
-
-
additional information
?
-
-
ompR-envZ is a two component regulatory system that plays an important role in the regulation of Vi polysaccharide synthesis in Salmonella typhi. One of the environmental signals for this regulation may be osmolarity
-
-
-
additional information
?
-
P0AEC7
E. coli BarA-UvrY two-component system is required for efficient switching between glycolytic and gluconeogenic carbon sources
-
-
-
additional information
?
-
-
purified BarA protein is able to autophosphorylate when incubated with [gamma-(32)P]ATP but not with [alpha-(32)P]ATP or [gamma-(32)P]GTP. Phosphorylated BarA, in turn, acts as an efficient phosphoryl group donor to UvrY. BarA and UvrY constitute a new two-component system for gene regulation in Escherichia coli
-
-
-
additional information
?
-
-
enzyme is involved in adaptive responses in E. coli
-
-
-
additional information
?
-
-
the VanR B-VanS B two-component regulatory system activates a promoter located immediately downstream from the vanS B gene
-
-
-
additional information
?
-
-
CpxA functions as a transmembrane sensory protein
-
-
-
additional information
?
-
-
EnvZ modulates expression of the ompF and ompC genes through phosphotransfer signal transduction in Escherichia coli
-
-
-
additional information
?
-
-
enzyme controls the osmoregulated biosynthesis of the porin proteins OmpF and OmpC
-
-
-
additional information
?
-
-
the enzyme plays a central role in osmoregulation, a cellular adaptation process involving the His-Asp phosphorelay signal transduction system. Dimerization of the transmembrane protein is essential for its autophosphorylation and phosphorelay signal transduction functions
-
-
-
additional information
?
-
-, O82436
Cm-ETR1 mRNA is very high in the seeds and placenta. Marked increase of Cm-ETR1 mRNA parallels climacteric ethylene production. Cm-ETR1 plays a specific role not only in ripening but also in the early development of melon fruit
-
-
-
additional information
?
-
-
the enzyme plays an important role in coupling signals received from membrane-bound receptors to changes in the swimming behavior of the cells in order to respond appropriately to environmental signals
-
-
-
additional information
?
-
-
a complex of the proteins CheA (CheAL and CheAS) and CheW constitutes a functional unit that responds to the signaling state of the chemoreceptors. The autophosphorylation rate of CheAL is much greater when CheAL and CheAS are complexed with CheW. Moreover, the presence of mutant chemoreceptors that cause cells to tumble increases this rate. At wild-type levels of expression, the isolated CheAL/CheAS/CheW complex accounts for about 10% of the total number of CheAL, CheAS, and CheW molecules and exists in a 1:1:1 stoichiometry. This complex is also required for CheAL/CheAS and CheW binding to the phosphorylation substrate, CheY
-
-
-
additional information
?
-
P07167, P07168
it is proposed that VirA acts as an environmental sensor of plant-derived inducer molecules and transmits this information to the level of vir gene expression
-
-
-
additional information
?
-
O31396
the two-component regulatory system irlR-irlS is involved in invasion of eukaryotic cells and heavy-metal resistance in Burkholderia pseudomallei
-
-
-
additional information
?
-
O32193
two-component regulatory system CssR-CssS, is required for the cell to survive the severe secretion stress caused by a combination of high-level production of the alpha-amylase AmyQ and reduced levels of the extracytoplasmic folding factor PrsA. The Css system is required to degrade misfolded exported proteins at the membrane-cell wall interface. CssS represents the first identified sensor for extracytoplasmic protein misfolding in a Gram-positive eubacterium
-
-
-
additional information
?
-
-
the CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis
-
-
-
additional information
?
-
-
the protein is involved in osmoregulation of OmpF and OmpC. EnvZ is considered to be an osmosensor which transmits signals across the membrane to OmpR, a transcriptional activator for ompF and ompC
-
-
-
additional information
?
-
-
UhpB and perhaps UhpC play both positive and negative roles in the control of uhpT transcription
-
-
-
additional information
?
-
P27668
UhpB and perhaps UhpC play both positive and negative roles in the control of uhpT transcription
-
-
-
additional information
?
-
-
regulation of nitrogen fixation genes in Rhizobium meliloti is mediated by two proteins, FixL and FixJ, in response to oxygen availability, oxygen sensor
-
-
-
additional information
?
-
-
the gene regulates transcription of the nifHDK operon and so limits the expression of nitrogen fixation activity to periods of low environmental concentrations of both oxygen and fixed nitrogen
-
-
-
additional information
?
-
-
FixL senses an environmental signal and transduces it to FixJ, a transcriptional activator of nif and fix genes
-
-
-
additional information
?
-
P10047
required for the activation of the C4-dicarboxylate transport structural gene dctA in free-living Rhizobium leguminosarum
-
-
-
additional information
?
-
P09384
during bacterial chemotaxis, the binding of stimulatory ligands to chemoreceptors at the cell periphery leads to a response at the flagellar motor. Three proteins appear to be required for receptor-mediated control of swimming behavior, the products of the cheA, cheW, and cheY genes
-
-
-
additional information
?
-
P08982
enzyme is required for the proper expression of the outer membrane proteins OmpC and OmpF
-
-
-
additional information
?
-
-
enzyme has an enhancing effect on the transcription of phoA, primary function may not be connected to the phosphate regulon
-
-
-
additional information
?
-
P10799
membrane-bound sensor of plant signal molecules
-
-
-
additional information
?
-
P18769
FrzE is a second messenger that relays information between the signaling protein FrzCD and the gliding motor
-
-
-
additional information
?
-
-
mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regulatory proteins
-
-
-
additional information
?
-
P14376
RcsC is the sensor components of the two-component regulatory system which regulates expression of the slime polysaccharide colanic acid. rcs system is essential for expression of high levels of the group I capsular polysaccharide in lon+ E. coli K30
-
-
-
additional information
?
-
-
the two-component regulatory system phoP/phoQ controls Salmonella typhimurium virulence
-
-
-
additional information
?
-
P14376
colanic acid capsule synthesis in Escherichia coli K-12 is regulated by RcsB and RcsC. RcsC acts as the sensor and RcsB acts as the receiver or effector to stimulate capsule synthesis from cps genes
-
-
-
additional information
?
-
-
bvgS and bvgA control the expression of the virulence-associated genes in Bordetella species by a system similar to the two-component systems used by a variety of bacterial species to respond to environmental stimuli
-
-
-
additional information
?
-
P14147
the PhoP-PhoQ system exerts a master regulatory function for preventing bacterial overgrowth within fibroblasts
-
-
-
additional information
?
-
P14377
the HydH/G system senses high periplasmic Zn2+ and Pb2+ concentrations and contributes to metal tolerance by activating the expression of zraP
-
-
-
additional information
?
-
Q9APE0
the HydH/G system senses high periplasmic Zn2+ and Pb2+ concentrations and contributes to metal tolerance by activating the expression of zraP
-
-
-
additional information
?
-
P15939
NodV and NodW proteins are members of the family of two-component regulatory systems, NodV responds to an environmental stimulus and, after signal transduction, NodW may be required to positively regulate the transcription of one or several unknown genes involved in the nodulation process
-
-
-
additional information
?
-
P20169
the enzyme is involved in chemical sensing
-
-
-
additional information
?
-
-
genes dctB and dctD form a two-component system which responds to the presence of C4-dicarboxylates to regulate expression of a transport protein encoded by dctA
-
-
-
additional information
?
-
-
narL and narX mediate nitrate induction of nitrate reductase synthesis and nitrate repression of fumarate reductase synthesis
-
-
-
additional information
?
-
-
in free-living cells, the regulatory dctBD genes are absolutely required for the expression of the dctA gene
-
-
-
additional information
?
-
-
dctB-encoded protein includes a putative periplasmic N-terminal domain that senses the presence of dicarboxylates and a C-terminal cytoplasmic domain that activates the dctD-encoded protein
-
-
-
additional information
?
-
-
enzyme is involved in signal transduction
-
-
-
additional information
?
-
-
the enzyme is a regulator of chemotaxis
-
-
-
additional information
?
-
P33113
enzyme is responsible for regulation of subtilin biosynthesis
-
-
-
additional information
?
-
-
enzyme is responsible for regulation of subtilin biosynthesis
-
-
-
additional information
?
-
-
enzyme is involved in the regulation of expression of phosphoglycerate transport in Salmonella typhimurium. pgtB and pgtC genes are involved in the induction of the pgtP expression by modulating derepressor activity
-
-
-
additional information
?
-
P29905
moxY is part of the two-component regulatory system controlling methanol dehydrogenase synthesis
-
-
-
additional information
?
-
-
either of two functionally redundant sensor proteins, NarX and NarQ, is sufficient for nitrate regulation in Escherichia coli K-12. NarQ and NarX may have subtle functional differences
-
-
-
additional information
?
-
-
activation role for ResD, and to a lesser extent ResE, in global regulation of aerobic and anaerobic respiration in Bacillus subtilis
-
-
-
additional information
?
-
P37433
PgtB and PgtC polypeptides modulate PgtA activity
-
-
-
additional information
?
-
-
narQ is a nitrate sensor for nitrate-dependent gene regulation in Escherichia coli
-
-
-
additional information
?
-
-
the enzyme is a biological oxygen sensors that restricts the expression of specific genes to hypoxic conditions
-
-
-
additional information
?
-
P21865
the two-component sensor-effector system KdpD /KdpE controls expression of the kdpABC operon
-
-
-
additional information
?
-
-
the two-component sensor-effector system KdpD /KdpE controls expression of the kdpABC operon
-
-
-
additional information
?
-
-
FixL and FixJ proteins are members of the two-component sensor/regulator family
-
-
-
additional information
?
-
P33639
PilS/PilR is a two-component transcriptional regulatory system controlling expression of type 4 fimbriae in Pseudomonas aeruginosa. PilS is a sensor protein which when stimulated by the appropriate environmental signals activates PilR through kinase activity. PilR then activates transcription of pilA, probably by interacting with RNA polymerase containing RpoN
-
-
-
additional information
?
-
-
the TorS/TorR two-component system induces the expression of the tor structural operon encoding the trimethylamine N-oxide reductase respiratory system in response to substrate availability. TorS belongs to a sensor subfamily that includes a classical transmitter domain, a receiver, and a C-terminal alternative transmitter domain
-
-
-
additional information
?
-
P49333
ETR1 acts as an ethylene receptor
-
-
-
additional information
?
-
-
ETR1 acts as an ethylene receptor
-
-
-
additional information
?
-
-
TorS is a sensor that contains three phosphorylation sites and transphosphorylates TorR via a four-step phosphorelation, His443--Asp723--His850--Asp(TorR)
-
-
-
additional information
?
-
P40719, Q8X524
QseBC is a two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli strains EHEC and K-12
-
-
-
additional information
?
-
-
TorS mediates the induction of the tor structural genes in response to trimethylamine N-oxide
-
-
-
additional information
?
-
-
enzyme is involved in positive regulation of synthesis of extracellular enzymes and polysaccharide in Xanthomonas campestris pathovar campestris
-
-
-
additional information
?
-
P45608
enzyme is involved in regulation of the phosphate regulon
-
-
-
additional information
?
-
P45609
enzyme is involved in regulation of the phosphate regulon
-
-
-
additional information
?
-
-
the two-component regulatory system DcuSR of Escherichia coli controls the expression of genes of C4-dicarboxylate metabolism in response to extracellular C4-dicarboxylates such as fumarate or succinate. The phosphoryl group of DcuS is rapidly transferred to the response regulator DcuR. Upon phosphorylation, DcuR binds specifically to dcuB promoter DNA
-
-
-
additional information
?
-
P52687
citrate, Na+, and oxygen exert their regulatory effects via the CitA/CitB system. In the presence of these signals, the citAB gene products induce their own synthesis. The positive autoregulation occurrs via co-transcription of citAB with citS and oadGAB
-
-
-
additional information
?
-
-
the genes encoding the anaerobic fumarate respiratory system are transcriptionally regulated by C4-dicarboxylates. The regulation is effected by a two-component regulatory system, DcuSR, consisting of a sensory histidine kinase DcuS and a response regulator DcuR
-
-
-
additional information
?
-
-
periplasmic loop of DcuS serves as a C4-dicarboxylate sensor. The cytosolic region of DcuS contains two domains: a central PAS domain possibly acting as a second sensory domain and a C-terminal transmitter domain
-
-
-
additional information
?
-
-
the two-component system regulates an osmosensing MAP kinase cascade
-
-
-
additional information
?
-
P77485
expression of cusC is induced by high concentrations of copper ions, the cusRS two-component signal transduction system is required for copper-induced expression of pcoE, a plasmid-borne gene from the E. coli copper resistance operon pco. The genes cusRS are also required for the copper-dependent expression of at least one chromosomal gene, designated cusC, which is allelic to the recently identified virulence gene ibeB in E. coli K1. The cus locus may comprise a copper ion efflux system
-
-
-
additional information
?
-
Q06067
the antizyme is a bifunctional protein serving as both an inhibitor of polyamine biosynthesis as well as a transcriptional regulator of an as yet unknown set of genes
-
-
-
additional information
?
-
-
the enzyme is involved in chemotaxis
-
-
-
additional information
?
-
Q02541
two-component regulatory system CopR/CopS is required for copper-inducible expression of the copper resistance operon
-
-
-
additional information
?
-
Q04850
the two-component regulatory system, NtrY/NtrX is involved in nitrogen fixation and metabolism. NtrY is likely to represent the transmembrane sensor protein element in a two-component regulatory system
-
-
-
additional information
?
-
Q04943
the two-component regulatory system afsQ1/afsQ2 is involved in secondary metabolism
-
-
-
additional information
?
-
P77510
the two-component systemDpiA/DpiB is involved in regulation of plasmid inheritance
-
-
-
additional information
?
-
P0A4I7
the cutR-cutS operon regulates copper metabolism in Streptomyces
-
-
-
additional information
?
-
P52687
rhe periplasmic domain of the histidine autokinase CitA functions as a highly specific citrate receptor
-
-
-
additional information
?
-
-
the ExoS-ChvI two-component regulatory system regulates succinoglycan production. ChvG is the sensor protein of the ChvG-ChvI two-component regulatory system
-
-
-
additional information
?
-
P0C5S6
the enzyme is involved in regulation of density-dependent expression of luminescence in Vibrio harveyi
-
-
-
additional information
?
-
P54302
the enzyme is involved in regulation of density-dependent expression of luminescence in Vibrio harveyi
-
-
-
additional information
?
-
-
the two-component regulatory system PfeR/PfeS is involved in the expression of the ferric enterobactin receptor PfeA
-
-
-
additional information
?
-
-
deletion of PilS results in a non-pilated phenotype
-
-
-
additional information
?
-
P0AEC8
a chemical or other stimulus is detected by the periplasmic sensor domain of a transmembrane histidine kinase sensor, which in turn relays a signal through a phosphotransfer cascade to the cognate cytoplasmic response regulator. Such systems lead ultimately to changes in gene expression or cell motility. Mechanisms of ligand binding and signal transduction through the cell membrane, overview
-
-
-
additional information
?
-
-, Q09JB2, Q09JB3, Q09JB4, Q09JB6, Q09JB7, Q09JB8, Q09JB9
AlHK1p is important for, but not the sole factor responsible for Alternaria longipes resistance to dicarboximide fungicides, e.g. dimethachlon, molecular basis of the fungicide resistance, overview
-
-
-
additional information
?
-
-
DcuS is the C4-dicarboxylate sensor of Escherichia coli catalyzing transmembrane sensing
-
-
-
additional information
?
-
-, Q81QX4
genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay
-
-
-
additional information
?
-
-
histidine kinases are part of two-component signal transduction systems that act to integrate environmental stimuli into a cellular response via a phosphotransfer relay mechanism, e.g. involved in stomatal guard cell response to H2O2, regulation of the physiological function, overview
-
-
-
additional information
?
-
Q95434
Myxococcus xanthus undergoes a complex starvation-induced developmental program that results in cells forming multicellular fruiting bodies by aggregating into mounds and then differentiating into spores. This developmental program involves EspA, which plays a key role in the timing of expression of genes necessary for progression of cells through the developmental program, overview
-
-
-
additional information
?
-
-
role of the C-terminal domains in the photocycle of a light sensor histidine kinase Ppr having a photoactive yellow protein, PYP, domain as the photosensor domain, photocycles of the PYP domain of Ppr and of the full-length Ppr. The, overview
-
-
-
additional information
?
-
Q9HWR3
signal transduction mechanism in the bacteriophytochrome, overview
-
-
-
additional information
?
-
Q88PG3
significant number of genes are influenced directly or indirectly by the RoxSR two-component system. 173 genes are found showing reduced expression in EU58 (RoxSR mutant strain) in comparison to the wild type, whereas 84 genes are upregulated in the mutant
-
-
-
additional information
?
-
Q25026
the enzyme HP0244 is not only required to regulate flagellar gene expression via its cognate response regulator, HP0703, but also to generate a response to declining medium pH. Although not required for survival at pH 4.5, HP0244 is required for survival at pH 2.5, overview
-
-
-
additional information
?
-
P16497
the N-terminal Per-ARNT-Sim domain plays a critical role in the catalytic activity of this enzyme, a significant decrease occurs of the autophosphorylation rate of a KinA protein lacking this domain
-
-
-
additional information
?
-
-
the SenS/SenR system of Streptomyces reticuli regulates the expression of the redox regulator FurS, the catalase-peroxidase CpeB and the heme-binding protein HbpS. SenS/SenR also participates in sensing redox changes, mediated by HbpS. The heme-binding protein HbpS regulates the activity of the Streptomyces reticuli iron-sensing histidine kinase SenS in a redox-dependent manner, presence of SenS/SenR leads to the synthesis of extracellular redox active proteins, overview
-
-
-
additional information
?
-
Q45614
The sensor histidine kinase YycG acts in a two-component system with response regulator/transcription factor YycF in Bacillus subtilis controling the synthesis of autolysins and autolysin inhibitors, that function in cell wall remodelling and cell separation, YycG sensor histidine kinase is a component of and perceives infirmation at the division septum in growing cells constituting a positive feedback loop, that serves to co-ordinate cell division with cell wall homeostasis, overview
-
-
-
additional information
?
-
-
the sensory histidine kinase acts in the two-component system with the response regulator as RR-HK17, EF1633-EF1632, in the regulation of ethanolamine utilization, which can be the sole carbon source for the organism, mechanism, overview
-
-
-
additional information
?
-
Q88PG3
The two-component system is implicated in redox signaling and cytochrome oxidase activity, in expression of the cell density-dependent gene ddcA and in bacterial colonization of plant surfaces. The RoxS/RoxR regulon includes genes involved in sugar and amino acid metabolism and the sulfur starvation response and elements of the respiratory chain (a cbb3 cytochrome oxidase, Fe-S clusters, and cytochrome c-related proteins) or genes participating in the maintenance of the redox balance. The RoxS/RoxR system controls a broad set of functions that have an influence on energy metabolism, such as formaldehyde and formate dehydrogenases.
-
-
-
additional information
?
-
Escherichia coli O9:K30:H12
P14376
RcsC is the sensor components of the two-component regulatory system which regulates expression of the slime polysaccharide colanic acid. rcs system is essential for expression of high levels of the group I capsular polysaccharide in lon+ E. coli K30
-
-
-
additional information
?
-
Agrobacterium tumefaciens 15955
P10799
membrane-bound sensor of plant signal molecules
-
-
-
additional information
?
-
Lactobacillus plantarum C11
-
DNA sequences of plnB reveals that the product closely resembles members of bacterial two-component signal transduction systems. The finding that plnABCD are transcribed from a common promoter suggests that the biological role played by the bacteriocin is somehow related to the regulatory function of the two-component system located on the same operon
-
-
-
additional information
?
-
Streptomyces reticuli Tue54
-
the SenS/SenR system of Streptomyces reticuli regulates the expression of the redox regulator FurS, the catalase-peroxidase CpeB and the heme-binding protein HbpS. SenS/SenR also participates in sensing redox changes, mediated by HbpS. The heme-binding protein HbpS regulates the activity of the Streptomyces reticuli iron-sensing histidine kinase SenS in a redox-dependent manner, presence of SenS/SenR leads to the synthesis of extracellular redox active proteins, overview
-
-
-
additional information
?
-
Bacillus anthracis 34F2DELTA118
Q81QX4
genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay
-
-
-
additional information
?
-
Bacillus subtilis 1A40
P16497
the N-terminal Per-ARNT-Sim domain plays a critical role in the catalytic activity of this enzyme, a significant decrease occurs of the autophosphorylation rate of a KinA protein lacking this domain
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
FAD
-
Slr1759-Var I protein contains 0.11-0.26 mM cofactor per 1 mM protein
flavin
-
flavin-containing histidine kinase functions as a photoreceptor, the flavin chromophore, upon illumination, forms a cysteinyl-flavin adduct, which is the signaling state that activates the histidine kinase, regulates Brucella abortus virulence
flavin
-
flavin-containing histidine kinase functions as a photoreceptor, the flavin chromophore, upon illumination, forms a cysteinyl-flavin adduct, which is the signaling state that activates the histidine kinase
GDP
-, Q81QX4
this kinase is uniquely specific for GTP in the forward and GDP in the reverse reaction. The G1 motif of BA2291 is highly modified from ATP specific histidine kinases, binding structure, modeling, overview
GTP
-, Q81QX4
this kinase is uniquely specific for GTP in the forward and GDP in the reverse reaction. The G1 motif of BA2291 is highly modified from ATP specific histidine kinases, binding structure, modeling, overview
heme
-
FixL is an oxygen-binding hemoprotein, the heme domain serves as the dioxygen switch in the FixL/FixJ two-component system
heme
-
the oxygen-detecting domain is a heme binding region that controls the activity of an attached histidine kinase. In the absence of bound ligand, the heme domain permits kinase activity. In the presence of bound ligand, this domain turns off kinase activity
additional information
-, Q81QX4
no activity with ATP, TTP, or CTP in autophosphorylation
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ag+
Q88KY2
specifically activated by silver
Cu2+
Q88KY2
specifically activated by 0.01 mM copper
Cu2+
-
essential cofactor
Fe
-
enzyme contains 2-labile 1-2 iron-sulphur [4Fe-4S]2+ clusters of the FNR-type
Fe2+
-
the presence of iron ions alone or in combination with H2O2 or dithiothreitol leads to significantly increased phosphorylation levels of SenS. 0.005 mM Fe2+ is able to enhance the phosphorylation level of SenS by a factor of 10 relative to the basal level, a combination of FeCl2 and H2O2 increases autophosphorylation activity by a factor of 20
Fe3+
-
0.005 mM Fe3+ is able to enhance the phosphorylation level of SenS by a factor of 10 relative to the basal level
FeCl2
-
enhances SenS autokinase activity in presence of HbpS, the activation is unaffected by DTT, but is enhanced by H2O2
FeCl3
-
enhances SenS autokinase activity strongly in presence of HbpS, H2O2 and DTT inhibit the activation
Mg2+
-
required for HK1 activity
additional information
-
the autokinase activity of SenS is not affeted by Zn2+, H2O2, and DTT
additional information
-
the autokinase activity of SenS is not affeted by Zn2+, H2O2, and DTT; the combination of 5 mM dithiothreitol and 0.005 mM FeCl2, or 5 mM dithiothreitol and 0.005 mM FeCl3, or 0.005 mM FeCl3 and 5 mM H2O2 has no effect on phosphorylation level of SenS, H2O2 prevents the activation by Fe3+; ZnCl2 is not able to change the autokinase activity of SenS
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(3-decanoyl-4,5,7-trihydroxynaphthalen-2-yl)acetic acid
-
IC50 of 0.0095 mg/ml
(4-[2-[4-(4-cyanobenzyl)-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl]ethoxy]phenyl)methanaminium chloride
-
-
2-(3,4-dimethoxyphenyl)-N-[(5-phenylfuran-2-yl)methyl]ethanaminium
Q9S1J9
-
2-(4-[2-[4-(4-chlorobenzyl)-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl]ethoxy]phenyl)ethanaminium chloride
-
-
2-(4-[2-[4-(4-cyanobenzyl)-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl]ethoxy]phenyl)ethanaminium chloride
-
-
2-[[(4-amino-1,2,5-oxadiazol-3-yl)carbonyl]amino]-N-[4-(thiophen-2-ylmethoxy)benzyl]ethanaminium
Q9S1J9
-
2-[[(4-amino-1,2,5-oxadiazol-3-yl)carbonyl]amino]-N-[[5-(3-chloro-4-methoxyphenyl)furan-2-yl]methyl]ethanaminium
Q9S1J9
-
3,6-diamino-5-cyano-4-phenyl-thieno[2,3-b]pyridine-2-carboxylic acid (4-bromo-phenyl)amide
-
competitive inhibitor of the coupled reaction of histidine kinase HpkA HK and the cognate response regulator DrrA RR
Art1
-
IC50 of 0.0095 mg/ml
cyanide
-
autokinase activity of the purified ethylene resistant 1 is completely abolished by 2 mM cyanide
ethylene
-
autokinase activity of the purified ethylene resistant 1 is completely abolished by 2 mM ethylene
Fe(II)-CO complex
-
the Fe(II)-CO complex of the heme nitric oxide/oxygen protein inhibits the autophosphorylation of the operon-associated histidine kinase
Fe(II)-NO complex
-
the Fe(II)-NO complex of the heme nitric oxide/oxygen protein inhibits the autophosphorylation of the operon-associated histidine kinase, whereas the ligand-free heme nitric oxide/oxygen protein has no effect on the kinase
HbpS
-
heme-free HbpS represses the autokinase activity of SenS
-
HCl
-
cytoplasmic phosphorylated AtoS is sensitive to treatment with 1 N HCl but stable in the presence of 1 N NaOH
heme-binding protein HbpS
-
inhibits autophosphorylation activity of the enzyme in absence of redox reagents and hemin, but activates slightly in presence of hemin and strongly in presence of FeCl2 and FeCl3, DTT increases the activation with FeCl2, but inhibits activation with FeCl3, while H2O2 strongly increases the activation by FeCl2 and completely inhibits activation with FeCl3, overview
-
kinase inhibitor protein
-
the kinase inhibitor protein KipI prevents sporulation by binding KinA and inhibiting the autophosphorylation reaction
-
N,N'-bis(1H-benzimidazol-2-yl)biphenyl-4,4'-diamine
Q9S1J9
-
N-[[5-(2-fluorophenyl)furan-2-yl]methyl]-3-[(1-methyl-1H-tetrazol-5-yl)sulfanyl]propan-1-aminium
Q9S1J9
-
N-[[5-(3-chloro-4-fluorophenyl)furan-2-yl]methyl]-3-[(1-methyl-1H-tetrazol-5-yl)sulfanyl]propan-1-aminium
Q9S1J9
-
Sda
-
potent inhibitor of KinB autophosphorylation, Sda directly inhibits sporulation histidine kinase in response to DNA damage and replication defects, Sda acts to sterically block communication between the catalytic ATP-binding and dimerization/histidinephosphotransfer domains required for autophosphorylation, as well as to sterically block communication between the response regulator Spo0F and DHp domain required for phosphotransfer and phosphatase activities
-
suppressor of dnaA
-
Sda, inhibits KinA by directly binding to the autokinase domain
-
Mg2+
-
PhoQ is strongly repressed in divalent cation-replete medium at 10 mM Mg2+
additional information
-
thermal transition of HK1 is a two-state process and that of HK2 is a three-state process. Urea denaturation of HK1 and HK2 is a three-state and two-state process, respectively
-
additional information
-
in the dark, roughly an order of magnitude fewer bacteria survive, a survival rate no better than that of the LOV-HK knockout mutant
-
additional information
-, Q81QX4
the virulence plasmid-encoded sensor domains of BA2291 have a regulatory effect in vivo on the activity of full-length BA2291, by converting it from a normally functional sporulation kinase to an enzyme that inhibits sporulation, molecular mechanism
-
additional information
-
not inhibited by Fe2+
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
alanine
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
auto-inducible protein I
-
dose-dependent activation by autoinducing peptide
-
betaine
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
citrate
-
effector of CitA
DivK
-
acts as a stimulator of both PleC and DivJ kinase activity
-
ethanolamine
-
induces a 15fold increase in the rate of autophosphorylation in vitro of the HK17 sensor histidine kinase
FixT
-
stimulates FixL-mediated dephosphorylation of FixJ
-
fructose
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
fumarate
-
effector of DcuS
glucose
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
glutamate
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
heme-binding protein HbpS
-
inhibits autophosphorylation activity of the enzyme in absence of redox reagents and hemin, but activates slightly in presence of hemin and strongly in presence of FeCl2 and FeCl3, DTT increases the activation with FeCl2, but inhibits activation with FeCl3, while H2O2 strongly increases the activation by FeCl2 and completely inhibits activation with FeCl3, overview
-
Hemin
-
activates slightly in presence of HbpS
L-glutamate
-
stimulates, more efficient stimulation of MtrB than MtrA
LprF
-
direct regulator of KdpD
-
LprJ
-
direct regulator of KdpD
-
lysine
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
maltoheptaose
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
maltose
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
NaCl
-
provides minor activation
proline
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
Ssl3451 protein
-
enhances the autophosphorylation activity of the histidine kinase Hik33 by associating with it with a 1:1 stoichiometry both in vitro and in vivo, but does not affect its dephosphorylation
-
Sucrose
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
trehalose
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
UhpC
-
UhpC stimulates UhpB autophosphorylation in the presence of D-glucose 6-phosphate
-
maltotriose
-
stimulates, stimulation pattern of MtrA is similar to that of MtrB
additional information
-
liposome shrinkage is not necessary for MtrB activation, MtrB is activated upon the addition of various chemical compounds, like sugars, amino acids, and polyethylene glycols, these may act via a change of the hydration state of the protein shifting MtrB into the active state, for activation it is essential that solutes are added at the same side as the cytoplasmic domains of the kinase are located
-
additional information
-
light-activated
-
additional information
-
light-activated, 2fold increase in transcription of the LOV-HK gene at a pH similar to that within infected macrophages
-
additional information
-
light-activated
-
additional information
-, Q81QX4
the virulence plasmid-encoded sensor domains of BA2291 have a regulatory effect in vivo on the activity of full-length BA2291, by converting it from a normally functional sporulation kinase to an enzyme that inhibits sporulation, molecular mechanism
-
additional information
-
hemin-treated HbpS enhances SenS autophosphorylation by 30fold under redox conditions using either H2O2 or dithiothreitol
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.1
-
ATP
-
mutant D657N/D782N/D909N
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.000167
-
ATP
-
mutant D657N/D782N/D909N
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00062
-
3,6-diamino-5-cyano-4-phenyl-thieno[2,3-b]pyridine-2-carboxylic acid (4-bromo-phenyl)amide
-
-
0.1
-
AMP-PNP
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.084
-
Fe(II)-CO complex
-
pH 8.0, 25C
0.009
-
Fe(II)-NO complex
-
pH 8.0, 25C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1.3
-
P16497
KinA autophosphorylation, purified recombinant mutant I95A
2.3
-
P16497
KinA autophosphorylation, purified recombinant wild-type enzyme
2.8
-
P16497
KinA autophosphorylation, purified recombinant mutant I108A
4
-
P16497
KinA autophosphorylation, purified recombinant mutant Y29A
additional information
-
-
activities of wild-type and truncated mutant SenS under different conditions, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.8
-
Q95434
assay at
7.5
-
-
assay at
7.5
-
P16497
assay at
7.5
-
-
assay at
7.5
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.4
8.1
-
the light/dark difference spectra of Ppr-PYP are pH sensitive, pH-dependent equilibrium change between Ppr-PYPL and Ppr-PYPM, overview
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
22
-
Q95434
assay at room temperature
22
-
-
assay at room temperature
25
-
P16497
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
histidine kinases of the organism show temporally and spatially different expression during the life cycle, e.g. expression of HK genes during sexual and asexual development, overview
Manually annotated by BRENDA team
Emericella nidulans ABPU1
-
histidine kinases of the organism show temporally and spatially different expression during the life cycle, e.g. expression of HK genes during sexual and asexual development, overview
-
Manually annotated by BRENDA team
Emericella nidulans ABPU1
-
-
-
Manually annotated by BRENDA team
Emericella nidulans ABPU1
-
conidial head
-
Manually annotated by BRENDA team
Q9XH57, Q9XH58
expressed in geranium florets long before they are receptive to pollination and transcript levels remain constant throughout floral development; expressed in geranium florets long before they are receptive to pollination and transcript levels remain constant throughout floral development
Manually annotated by BRENDA team
-, Q9ZWL6
the level of expression of PE-ETR1 does not significantly change over the course of ripening, however, the mRNA levels of PE-ETR1 is much higher in arils than in seeds
Manually annotated by BRENDA team
-
; expressed at low levels in guard cells
Manually annotated by BRENDA team
Emericella nidulans ABPU1
-
-
-
Manually annotated by BRENDA team
O49187
LeETR2 mRNA expression is down-regulated in senescing leaf petioles
Manually annotated by BRENDA team
-, O82436
Cm-ETR1 mRNA is very high
Manually annotated by BRENDA team
O49187
LeETR2 mRNA is expressed at low levels throughout the plant but is induced in imbibing seeds prior to germination
Manually annotated by BRENDA team
-, O82436
Cm-ETR1 mRNA is very high
Manually annotated by BRENDA team
O49187
LeETR2 mRNA expression is down-regulated in elongating seedlings
Manually annotated by BRENDA team
-
vascular tissues of inflorescence stems
Manually annotated by BRENDA team
-
RodK is present in vegetative cells and remains present until the late aggregation stage, after which the level decreases in a manner that depends on the intracellular A-signal
Manually annotated by BRENDA team
additional information
O49187
LeETR1 is expressed constitutively in all plant tissues examined
Manually annotated by BRENDA team
additional information
-
FrzE is clearly present during vegetative growth and at much lower levels during development
Manually annotated by BRENDA team
additional information
-
mRNA is constitutively expressed in vegetative and reproductive tissues
Manually annotated by BRENDA team
additional information
Q95434
EspA developmental expression pattern, mutational analysis, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Vibrio cholerae MO45
-
-
-
-
Manually annotated by BRENDA team
-
RodK is a soluble cytoplasmic protein, which contains an N-terminal sensor domain, a histidine protein kinase domain and three receiver domains
Manually annotated by BRENDA team
Q57BR6
is localized at the old pole of the large cell, after division and growth, the small cell acquires PdhS at its old pole, polar localization of PdhS is maintained during a cellular infection by bovine macrophages
Manually annotated by BRENDA team
Brucella abortus XDB1104
-
is localized at the old pole of the large cell, after division and growth, the small cell acquires PdhS at its old pole, polar localization of PdhS is maintained during a cellular infection by bovine macrophages
-
Manually annotated by BRENDA team
-
enzyme is anchored to the cytoplasmic membrane by the amino-terminal region
-
Manually annotated by BRENDA team
-
structure-function relationship in the cytoplasmic PAS domain, the multidomain protein DcuS possesses functional domains in the periplasm, within the membrane and in the cytoplasm, the localization depends on the functional state, overview
Manually annotated by BRENDA team
Staphylococcus carnosus M1
-
-
-
Manually annotated by BRENDA team
-
ETR1 of Arabidopsis contains transmembrane domains responsible for ethylene binding and membrane localization
Manually annotated by BRENDA team
-
the enzyme is distributed evenly about the membrane of Escherichia coli
-
Manually annotated by BRENDA team
-
bound, PilS is retained to the poles of Pseudomonas aeruginosa
-
Manually annotated by BRENDA team
-
EnvZ with a N-terminal cytoplasmic tail (residues 1-15), two transmembrane domains (residues 16-34 and residues 163-179) flanking a periplasmic domain (residues 48-162), and a cytoplasmic domain (residues 180-450). The cytoplasmic domain can be further dissected into a linker or HAMP domain (residues 180-222), domain A (dimerization and histidine containing domain, residues 223-289) and domain B (catalysis assisting and ATP binding domain, residues 290-450), UhpB contains eight transmembrane segments
-
Manually annotated by BRENDA team
O34206
NH2-terminal periplasmic domain
Manually annotated by BRENDA team
-
cytoplasmic side of inner membrane
Manually annotated by BRENDA team
-
EnvZ is a transmembrane protein with histidine kinase activity in its cytoplasmic region. The cytoplasmic region contains two functional domains: domain A, residues 223-289, contains the conserved histidine residue H243, a site of autophosphorylation as well as transphosphorylation to the conserved D55 residue of response regulator OmpR
Manually annotated by BRENDA team
-
transmembrane regions of EnvZ play roles in transmembrane signaling
Manually annotated by BRENDA team
-
transmembrane protein
Manually annotated by BRENDA team
-
the region between the hydrophobic segments of CpxA is periplasmic, whereas the region carboxy-terminal to the second such segment is cytoplasmic. CpxA functions as a trans-membrane sensory protein
Manually annotated by BRENDA team
-
may be a membrane protein
Manually annotated by BRENDA team
-
a large periplasmic domain is lacking and an extended cytoplasmic domain is present besides the kinase domain
Manually annotated by BRENDA team
-
EnvZ contained two hydrophobic stretches typical of transmembrane regions
Manually annotated by BRENDA team
P10047
N-terminal region may be located in the periplasm and its C-terminal region in the cytoplasm
Manually annotated by BRENDA team
-
FixL has features of a transmembrane protein
Manually annotated by BRENDA team
-
FixL is a membrane protein containing four possible transmembrane segments
Manually annotated by BRENDA team
-
dctB-encoded protein includes a putative periplasmic N-terminal domain that senses the presence of dicarboxylates and a C-terminal cytoplasmic domain that activates the dctD-encoded protein
Manually annotated by BRENDA team
-
transmembrane protein
Manually annotated by BRENDA team
-
KdpD is anchored to the membrane by four membrane-spanning segments near its middle, with both C-terminal and N-terminal portions in the cytoplasm
Manually annotated by BRENDA team
-
KdpD has four membrane-spanning segments in the middle of the polypeptide chain, whereas N and C terminus are both cytoplasmic
Manually annotated by BRENDA team
-
membrane-bound protein comprising at least three cytoplasmic domains
Manually annotated by BRENDA team
P37739
membrane-bound sensor-kinase with two potential membrane-spanning sequences in the N-terminal region
Manually annotated by BRENDA team
-
DcuS contains two putative transmembrane helices flanking an approximately 140-residue N-terminal domain apparently located in the periplasm
Manually annotated by BRENDA team
-
DcuS is a membrane-integral sensor kinase, and the sensory and kinase domains are located on opposite sides of the cytoplasmic membrane
Manually annotated by BRENDA team
-
CitA contains in the N-terminal half, two putative transmembrane helices which enclosed a presumably periplasmic domain of about 130 amino acids
Manually annotated by BRENDA team
-
CitA represents a membrane-bound sensor kinase consisting of a periplasmic domain flanked by two transmembrane helices, a linker domain and the conserved kinase or transmitter domain
Manually annotated by BRENDA team
Q04850
NtrY is likely to represent the transmembrane sensor protein element in the two-component regulatory system
Manually annotated by BRENDA team
-
transmembrane enzyme
Manually annotated by BRENDA team
-
FixL contains four transmembrane segments
Manually annotated by BRENDA team
-
Hik33 perceives the cold signal via rigidification of membrane lipids
Manually annotated by BRENDA team
-
membrane-integral protein, DcuS and CitA accumulate at the cell poles
Manually annotated by BRENDA team
-
integral membrane protein
Manually annotated by BRENDA team
Agrobacterium tumefaciens 15955
-
-
-
Manually annotated by BRENDA team
-
the multidomain protein DcuS possesses functional domains in the periplasm, within the membrane and in the cytoplasm, the localization depends on the functional state, overview
-
Manually annotated by BRENDA team
-
the multidomain protein DcuS possesses functional domains in the periplasm, within the membrane and in the cytoplasm, the localization depends on the functional state, overview
Manually annotated by BRENDA team
Streptomyces reticuli Tue54
-
;
-
Manually annotated by BRENDA team
additional information
P0AEC8
periplasmic sensor domains
-
Manually annotated by BRENDA team
additional information
-
periplasmic sensor domains
-
Manually annotated by BRENDA team
additional information
Q45614
YycG is located at the division septum in growing cells, YycG localization is dependent upon FtsZ, but independent of YycH and YycI
-
Manually annotated by BRENDA team
additional information
Vibrio cholerae MO45
-
periplasmic sensor domains
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
Burkholderia pseudomallei (strain 1710b)
Burkholderia pseudomallei (strain K96243)
Burkholderia pseudomallei (strain K96243)
Burkholderia pseudomallei (strain K96243)
Burkholderia pseudomallei (strain K96243)
Caulobacter crescentus (strain ATCC 19089 / CB15)
Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809)
Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath)
Nostoc sp. (strain PCC 7120 / UTEX 2576)
Polaromonas sp. (strain JS666 / ATCC BAA-500)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain UCBPP-PA14)
Pseudomonas syringae pv. tomato (strain DC3000)
Rhizobium meliloti (strain 1021)
Rhizobium meliloti (strain 1021)
Rhizobium meliloti (strain 1021)
Rhizobium meliloti (strain 1021)
Rhizobium meliloti (strain 1021)
Rhizobium meliloti (strain 1021)
Rhizobium meliloti (strain 1021)
Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
Rhodopseudomonas palustris (strain TIE-1)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Salmonella typhimurium (strain SL1344)
Salmonella typhimurium (strain SL1344)
Shewanella oneidensis (strain MR-1)
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Synechococcus elongatus (strain PCC 7942)
Synechococcus elongatus (strain PCC 7942)
Synechococcus sp. (strain JA-2-3B'a(2-13))
Synechococcus sp. (strain JA-2-3B'a(2-13))
Synechococcus sp. (strain JA-2-3B'a(2-13))
Synechocystis sp. (strain PCC 6803 / Kazusa)
Synechocystis sp. (strain PCC 6803 / Kazusa)
Syntrophus aciditrophicus (strain SB)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
31500
-
-
truncated His-tagged RaxH, predicted
46000
-
-
YbdK-transmembrane domain dodecyl-phosphocholine complex, solution state NMR T1/T2 relaxation analysis
48900
-
-
His-tagged RaxH, predicted
50000
-
Q2GJD7
SDS-PAGE
51000
-
Q9X180
delta517ThkA, experimental estimates
75000
-
P39453
gel filtration
75000
-
-
gel filtration
76000
-
Q9X180
delta408ThkA, experimental estimates
88000
-
-, Q81QX4
about, gel filtration
130000
-
Q66WP9
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 102452, calculation from nucleotide sequence
?
O34206
x * 66000
?
-
x * 50000
?
P06218
x * 38409, calculation from nucleotide sequence
?
-
x * 52000
?
-
x * 49666
?
-
x * 49772, calculation from nucleotide sequence
?
-
x * 67275, calculation from nucleotide sequence
?
-
x * 69170
?
-
x * 83000
?
-
x * 74500
?
-
x * 99000
?
-
x * 55290
?
-
x * 99000, SDS-PAGE
?
-
x * 44600, calculation from nucleotide sequence
?
P39664
x * 46389, calculation from nucleotide sequence
?
-
x * 48846, calculation from nucleotide sequence
?
P54663
x * 43800
?
-
x * 50597, calculation from nucleotide sequence
?
-
x * 47774, calculation from nucleotide sequence
?
-
x * 54000, SDS-PAGE of MtrB
?
Q95434
x * 83000, SDS-PAGE
dimer
-
2 * 79000
dimer
-
the Cph1 protein forms dimers through the C-terminal region
dimer
Q9X180
crystallography
dimer
P16497
wild-type KinA PAS-A, NMR, SDS-PAGE and gel filtration
dimer
-, Q81QX4
2 * 44000, SDS-PAGE
dimer
-
the signal transduction histidine kinase domain, H-NOXA revealing a Per-Arnt-Sim fold, monomers dimerize in a parallel arrangement juxtaposing their N-terminal helices and preceding residues, overview
dimer
Q9HWR3
dimeric PaBphP-PCD structure with PAS, GAF, and PHY domains, interdomain and histidine kinase domain, domain structure, overview
dimer
P39453
the isolated TorS sensor domain dimerizes in solution
dimer
-
the isolated TorS sensor domain dimerizes in solution
dimer
-
SDS-PAGE
dimer
-
x-ray crystallography
dimer
-
x-ray crystallography
dimer
-
AgrC forms ligand-independent dimers that undergo trans-autophosphorylation upon interaction with autoinducing peptide
dimer
Bacillus anthracis 34F2DELTA118
-
2 * 44000, SDS-PAGE
-
dimer
Bacillus subtilis 1A40
-
wild-type KinA PAS-A, NMR, SDS-PAGE and gel filtration
-
dimer
Vibrio parahaemolyticus EB101
-
the isolated TorS sensor domain dimerizes in solution
-
homodimer
-
YbdK-transmembrane domain forms homodimers in dodecyl-phosphocholine micelles, cross-linking assay and analytical ultracentrifugation analysis
homodimer
O34757
x-ray crystallography
monomer
Q749I7
gel filtration
monomer
P0AFA2
gel filtration
additional information
-
the periplasmic region of histidine kinase EnvZ(Ala38-Arg162) forms a dimer in solution
additional information
P16497
determination of the importance of several residues at the dimer interface for KinA enzymatic activity in vitro and in vivo, KinA architecture and domain structure, and PAS-A domain organization and secondary structure, overview
additional information
-
the N-terminus of HK17 forms a GAF domain, structure of the RR-HK17 two-component system
additional information
-
the N-terminal domain in an STHK from Nostoc punctiforme is homologous to the central region in soluble guanylyl cyclase, the main receptor for nitric oxide, structure-function analysis and dimerization mechanism, overview
additional information
P0AEC8
DcuS shows a mixed alpha/beta-structure containing two subdomains of similar folds, each consisting of a five-stranded antiparallel beta-sheet flanked by helices on either side, structure analysis, residues 42-181 comprise the sensory domain of the enzyme, dimerization of DcuS-42181, overview
additional information
-
DctB shows a mixed alpha/beta-structure containing two subdomains of similar folds, each consisting of a five-stranded antiparallel beta-sheet flanked by helices on either side, structure analysis, residues 28-286 comprise the sensory domain of the enzyme, overview
additional information
-
NMR structure analysis, localization and structural model, detailed overview
additional information
-
Ppr is composed of three domains, a PYP domain, a bacteriophytochrome Bph domain, and a histidine kinase domain, in the order from the N- to the C-terminal of Ppr
additional information
Q9HWR3
modeling of the full-length bacteriophytochrome structure, including its output histidine kinase domain, structure-function relationship, overview
additional information
Bacillus subtilis 1A40
-
determination of the importance of several residues at the dimer interface for KinA enzymatic activity in vitro and in vivo, KinA architecture and domain structure, and PAS-A domain organization and secondary structure, overview
-
additional information
Vibrio cholerae MO45
-
DctB shows a mixed alpha/beta-structure containing two subdomains of similar folds, each consisting of a five-stranded antiparallel beta-sheet flanked by helices on either side, structure analysis, residues 28-286 comprise the sensory domain of the enzyme, overview
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
phosphoprotein
-
autophosphorylation
phosphoprotein
-, Q81QX4
BA2291 performs GTP-dependent autophosphorylation
phosphoprotein
Bacillus anthracis 34F2DELTA118
-
BA2291 performs GTP-dependent autophosphorylation
-
phosphoprotein
-
autophosphorylation
phosphoprotein
P16497
KinA performs autophosphorylation
phosphoprotein
Bacillus subtilis 1A40
-
KinA performs autophosphorylation
-
phosphoprotein
-
autophosphorylation
phosphoprotein
-
-
phosphoprotein
-
phosphorylation of CckA is required for function but is not important for polar localization
phosphoprotein
-
-
phosphoprotein
-
the enzyme HK17 performs autophosphorylation stimulated by ethanolamine
phosphoprotein
-
His243 is the major site of phosphorylation on EnvZ
phosphoprotein
-
autophosphorylation; enzyme undergoes phosphorylation in the presence of ATP
phosphoprotein
-
purified BarA protein is able to autophosphorylate when incubated with [gamma-(32)P]ATP but not with [alpha-(32)P]ATP or [gamma-(32)P]GTP. Phosphorylated BarA, in turn, acts as an efficient phosphoryl group donor to UvrY. BarA and UvrY constitute a new two-component system for gene regulation in Escherichia coli
phosphoprotein
-
autophosphorylation; His243 is the a site of autophosphorylation as well as transphosphorylation to the conserved D55 residue of response regulator OmpR
phosphoprotein
-
autophosphorylation; the major site at which NRII is autophosphorylated is contained within a peptide consisting of amino acid residues 136-142 of NRII, and thus probably corresponds to H139. A minor site of phosphorylation, accounting for about 2% of the phosphate in NRII-P, is found in a peptide that corresponds to residues 158-169
phosphoprotein
-
the phosphotransfer domain, CheA1-134, contains the site of phosphorylation, His48, and two other histidine residues, His26 and His67
phosphoprotein
-
autophosphorylation
phosphoprotein
-
autophosphorylation, histidine-398 in the conserved H-box is the phospho-accepting site
phosphoprotein
-
-
phosphoprotein
-
autophosphorylation in the presence of ATP
phosphoprotein
-
low pH triggers the autophosphorylation of the histidine kinase ArsS
phosphoprotein
-
low pH triggers the autophosphorylation of the histidine kinase ArsS
-
phosphoprotein
-
autophosphorylation
phosphoprotein
Q95434
the enzyme autophosphorylates in vitro on the conserved histidine residue and then transfers the phosphoryl group to the conserved aspartate residue in the associated receiver domain
phosphoprotein
-
FrzCD stimulates FrzE autophosphorylation
phosphoprotein
O34206
autophosphorylation; in the presence of [gamma-32P]ATP, the purified COOH-terminal KinB protein undergoes progressive autophosphorylation in vitro. Substitutions of the residues conserved among histidine protein kinases abolishes KinB autophosphorylation
phosphoprotein
-
histidine kinases autophosphorylate prior to transferring the phosphate to a response regulator
phosphoprotein
-
-
phosphoprotein
-
AgrC forms ligand-independent dimers that undergo trans-autophosphorylation upon interaction with autoinducing peptide
phosphoprotein
-
SenS performs autophosphorylation
phosphoprotein
-
; SenS performs autophosphorylation
phosphoprotein
Streptomyces reticuli Tue54
-
; SenS performs autophosphorylation; SenS performs autophosphorylation
-
phosphoprotein
-
autophosphorylation
phosphoprotein
-
the Slr1759 histidine kinase domain undergoes autophosphorylation in vitro
phosphoprotein
-
autophosphorylation
phosphoprotein
-
-
phosphoprotein
Q66WP9
-
additional information
-
the enzyme performs regulatory autophosphorylation
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging drop vapor diffusion method, using 2.54 M ammonium sulfate, 15.9% (v/v) glycerol, and 0.1 M MES buffer pH 5.05
-
purified recombinant residues 10-117 of KinA, fused to an N-terminal His6Gbeta1-tag and a TEV protease site in the tag-protein linker, free or as selenomethionine-tagged variant, hanging-drop vapor-diffusion method, 20C, mixing of 0.001 ml 7 mg/mL protein solution containing 25 mM Tris pH 8.0, 100 mM NaCl, with 0.001 ml well solution containing 13-15% w/v PEG 10000, 0.1 M ammonium acetate, and 0.1 M bis-Tris, pH 5.5, and with 20 mM DTT in case of the selenomethionine-labeled protein, 4 days, cryoprotetion using 25% v/v glycerol, X-ray diffraction structure determination and analysis at 1.7-2.0 A resolution
P16497
the phosphorylated cytoplasmic domain of DesK is crystallized by hanging drop vapor diffusion, using 10% (w/v) PEG 3000, 0.1 M CHES, pH 9.5, and 10 mM MgCl2, at 18C
O34757
crystal structure at 2.0 A resolution of the complex of the Escherichia coli chemotaxis response regulator CheY and the phosphoacceptor-binding domain P2 of the kinase CheA
-
crystal structure of the C-terminal HPt domain of ArcB
-
crystal structure of the histidine-containing phosphotransfer domain
-
crystal structure, at 2.95 A resolution, of the response regulator of bacterial chemotaxis, CheY, bound to the recognition domain from its cognate histidine kinase, CheA
-
crystallization of a complex between a novel C-terminal transmitter, HPt domain, of the anaerobic sensor kinase ArcB and the chemotaxis response regulator CheY
-
free and in complex with nitrate, hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5) and 2 M NH4H2PO4 at 4C (enzyme in complex with nitrate) or using 23% (w/v) polyethylene glycol 3350, 1% (v/v) isopropanol, and 0.1 M HEPES (pH 7.5) at 4C (free apo-enzyme)
P0AFA2
hanging drop vapor diffusion method, at 4C against a buffer containing 5%10% (v/v) 1-4 butanediol, 0.1 M Na-aetate (pH 5.5), 25100 mM NH4SO4
P39453
purified recombinant selenomethionine-labeled and wild-type GST-tagged DcuS residues 42-181 in complex with C4-dicarboxylate ligand, 18.5 mg/ml protein, hanging-drop vapor diffusion, using a reservoir buffer containing 20-24% PEG monoethyl ether 2000, 15% isopropyl alcohol, 0.2 M ammonium citrate, and 0.1 M sodium acetate, pH 4.5, at 4 C, 1 week, X-ray diffraction structure determination and analysis
P0AEC8
in complex with inhibitor Sda, crystals only form in the presence of ADP-Mg2+ (but not with ATP or AMPPNP, and not in the absence of nucleotide)
-
hanging drop vapor diffusion method, using 8% (v/v) isopropanol, 2% (w/v) PEG 3350, 150 mM calcium acetate, and 100 mM MES, pH 6.0, at 4C
Q749I7
citrate-free or citrate-bound CitA, hanging drop vapor diffusion method, using either 0.1 M HEPES, pH 7.5, 1.6 M (NH4)2SO4, and 5 mM sodium citrate or using 20 mM HEPES, pH 7.5, 0.63 M NaH2PO4 and 0.63 M KH2PO4
-
hanging drop vapor diffusion method
O53473
dimerized signal transduction histidine kinase domain H-NOXA domain residues 8-121, 4C, sitting-drop vapor diffusion, 20 mg/ml NpSTHK8121 in 5 mM Tris, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, is mixed with an equal volume of reservoir solution containing 1.7-1.9 M ammonium sulfate, 100 mM Tris-HCl, pH 7.7-9.0, for crystallization of the full-length enzyme, 15 mg/ml protein is mixed with equal volume of 0.1 M HEPES, pH 7.5, and 1.5 M lithium sulfate monohydrate, crystals appear after 3 days at 20C, X-ray diffraction structure determination and analysis at 2.1 A resolution
-
wild-type and mutant bacteriophytochrome with an intact, fully photoactive photosensory core domain in its darkadapted Pfr state, X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular replacement
Q9HWR3
ThkA complexed with the response regulator TrrA, by the batch method, X-ray crystallography at a resolution of 4.2 A, 2fold symmetry, two monomeric TrrAs bind to the ThkA dimer
Q9X180
recombinant selenomethionine-labeled DctB residues 28-286 in complex with C4-dicarboxylate ligand, by hanging-drop vapor diffusion at 4C against a buffer containing 6% isopropyl alcohol, 0.2 M calcium acetate, and 0.1 M Tris-HCl, pH 7.5, the protein concentration is 8.5 mg/ml with a protein to buffer ratio of 1:1, 2 weeks, X-ray diffraction structure determination and analysis
-
hanging drop vapor diffusion method, at 4C against a buffer containing 7%13% (w/v) PEG 4000, 0.1 M Tris (pH 7.5)
-
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
-
HK2 is stable up to 40C and reaches an intermediate state at 60C. On raising the temperature to 100C, the second stage of denaturation is observed
60
-
-
HK1 relatively stable up to 60C, gets completely denatured at 90C
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, 50 mM Tris/HCl buffer, pH 8.0, 500 mM NaCl, 10% glycerol, 10 mM beta-mercaptoethanol
-
-20C, 50 mM Tris-HCl buffer, pH 7.650 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 2530% glycerol
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
amylose resin column chromatography
Q2GJD7
HiTrap Zn2+-chelating column chromatography, Superdex 75 gel filtration
-
Ni-NTA column chromatography and G25 gel filtration
-
recombinant His6-tagged BA2291 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-, Q81QX4
DEAE-agarose column chromatography, Ni-NTA column chromatography, glutathione-agarose resin column chromatography, and Superdex 200 gel filtration
-
Ni-NTA column chromatography and gel filtration
-
Ni2+ affinity column chromatography and Superdex 75 gel filtration
O34757
Ni2+-NTA agarose column chromatography
-
cytoplasmic portion of BvgS ('BvgS)
-
on bialaphos selective medium
-
full-length protein affinity-purified
-
Talon metal affinity resin chromatography
-
by gel filtration
-
full-length protein affinity-purified
-
HiTrap Ni2+-chelating column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration
P39453
IMAC and Ni2+-NTA agarose column chromatography
-
Ni2+-affinity column chromatography, MonoQ column chromatography, glutathione Sepharose column chromatography, Superdex 200 gel filtration, and Superdex 75 gel filtration
P0AFA2
recombinant periplasmic domain of histidine kinase EnvZ(Ala38-Arg162)
-
recombinant selenomethionine-labeled GST-tagged DcuS residues 42-181 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and gel filtration
P0AEC8
Ni2+-affinity column chromatography, followed by anion exchange chromatography and gel filtration
-
glutathione Sepharose column chromatography and Superdex 75 gel filtration
Q749I7
by Ni-NTA affinity column, HK1 purified to homogeneity
-
by Ni-NTA column and gel filtration
-
HisTrap HP column chromatography
O53473
HisTrap column chromatography
-
recombinant FrzE protein is overproduced in Escherichia coli and purified from inclusion bodies
-
histidine kinase NTHK2
O48929
recombinant His-tagged 1-121 and truncated DELTA7 H-NOXA domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
a KinB derivative containing the COOH terminus of KinB
O34206
full-length protein affinity-purified
-
Ni-chelating Sepharose column chromatography and Sephacryl S300 gel filtration; recombinant His10-tagged full-length wild-type Ppr from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
Ni-NTA resin column chromatography and S200 HiLoad resin gel filtration
-
Ni-NTA resin column chromatography, Q-Sepharose resin column chromatography, and Superdex 75 gel filtration
-
Ni2+-NTA column chromatography, gel filtration; recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
coexpressed with a bacterial thioredoxin in Escherichia coli
-
HiTrap chelating column chromatography
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and glutathione Sepharose column chromatography
-
recombinant
-
by gel filtration
Q9X180
recombinant selenomethionine-labeled DctB residues 28-286 from Escherichia coli strain BL21(DE3) by anion exchange chromatography and gel filtration, followed by a second step of anion exchange chromatography
-
HiTrap Ni2+-chelating column chromatography, Mono Q column chromatography, and Superdex 75 gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
subcloning of the entire vir regulon
-
expressed in Saccharomyces cerevisiae
Q66WP9
cloning of gene AlHK1 from 19 different isolates, DNA and amino acid sequence determination and analysis, sequence comparisons; cloning of gene AlHK1 from 19 different isolates, DNA and amino acid sequence determination and analysis, sequence comparisons; cloning of gene AlHK1 from 19 different isolates, DNA and amino acid sequence determination and analysis, sequence comparisons; cloning of gene AlHK1 from 19 different isolates, DNA and amino acid sequence determination and analysis, sequence comparisons; cloning of gene AlHK1 from 19 different isolates, DNA and amino acid sequence determination and analysis, sequence comparisons; cloning of gene AlHK1 from 19 different isolates, DNA and amino acid sequence determination and analysis, sequence comparisons; cloning of gene AlHK1 from 19 different isolates, DNA and amino acid sequence determination and analysis, sequence comparisons
-, Q09JB2, Q09JB3, Q09JB4, Q09JB6, Q09JB7, Q09JB8, Q09JB9
expressed in Escherichia coli BL21(DE3) cells
Q2GJD7
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli strains BL21(DE3), C41 (DE3), and C43 (DE3)
-
gene ahk5, transient expression of GST-tagged full-length AHK5 in Arabidopsis thaliana protoplasts and Nicotiana benthamiana leaf cells the fusion protein in the cytoplasm, independent of whether the GFP tag is fused to the N-terminus or C-terminus of AHK5, expression profile of AHK5 in different tissues and cell types by semi-quantitative RT-PCR
-
kanamycin cartridges are inserted into the cloned fixL gene and recombined into the host genome
-
expression in Bacillus subtilis
-
gene BA2291, genes for orthologs of the sensor domain of the BA2291 kinase exist in virulence plasmids in this organism, and these proteins, when expressed, inhibit sporulation by converting BA2291 to an apparent phosphatase of the sporulation phosphorelay. Expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-, Q81QX4
expressed in Escherichia coli
O34757
expressed in Escherichia coli BL21(DE3) cells
-
expression of residues 10-117 of KinA, fused to an N-terminal His6Gbeta1-tag and a TEV protease site in the tag-protein linker, in Escherichia coli strain BL21(DE3), expresssion of the selenomethionine variant in Escherichia coli strain B834
P16497
expression of these proteins from a multicopy plasmid vector in Escherichia coli
Q08408
cytoplasmic portion of BvgS ('BvgS) is overexpressed
-
pBOS1delta linearized and used to transform protoplasts of the UWS111 wild-type strain
-
low-copy plasmid pRH324 expressing Brucella abortus pdhS fused to cfp into Sinorhizobium meliloti or Caulobacter crescentus
Q57BR6
LOV-HK expressed in Escherichia coli
-
expressed in Escherichia coli C41(DE3) cells
-
Escherichia coli BL21(DE3) cells transformed with different pASK-IBA7-mtrB strep derivatives
-
genes phkA, tcsB, nikA, fphA, hk-8-2, and hk-8-5, expression of GFP-tagged enzyme in Aspergillus nidulans strain ABPU1 under the control of an HK promoter, subcloning in Escherichia coli strain JM109, analysis of the temporally and spatially different expression during the cell cycle, overview
-
cloning of the RR-HK17 TCS system from strain V583
-
EL368-LOV-HK and EL346-LOV-HK expressed in Escherichia coli
-
cytoplasmic expression of TorSS as a fusion protein, containing a hexahistidine tag on the N-terminus separated by a separated by a thrombin protease recognition sequence, in Escherichia coli BL21(DE3) cells
P39453
expressed in Escherichia coli BL21(DE3) cells
-
expression of the periplasmic domain of histidine kinase EnvZ(Ala38-Arg162) in Escherichia coli
-
mutant enzymes cloned and expressed in Escherichia coli strain BL21(DE3)
-
overexpression of a 36-kDa truncated EnvZ protein, Glu106 to Gly450, that forms inclusion bodies in the cell
-
phoP-phoQ operon cloned and expressed in Escherichia coli
-
expression of selenomethionine-labeled DcuS residues 42-181 in Escherichia coli strain BL21(DE3) as GST-tagged protein
P0AEC8
expressed in Escherichia coli Rosetta2 DE3 cells
-
expressed in Escherichia coli BL21(DE3) cells
Q749I7
into vector pGEM-T, then cloned genes disrupted in HP0244, HP1364, and HP0165 sequences. The resulting plasmids, which are unable to replicate in Helicobacter pylori, introduced into Helicobacter pylori J99
-
profiling of a HP0244 deletion mutant grown at pH 7.4, contribution of HP0244 to sigma(54) activation via HP0703 to coordinate flagellar biosynthesis by a pH-independent regulon that includes 14 flagellar genes. Microarray analysis of cells grown at pH 4.5 without urea reveals an additional 22 genes, including 4 acid acclimation genes, e.g. ureA, ureB, ureI, and amiE, that are positively regulated by HP0244, overview
Q25026
expressed in Escherichia coli BL21(DE3) cells
-
heterologous expression of the plnABCD operon in a Lactobacillus sake strain
-
expression of Hik1 can confer fungicide-sensitivity to Saccharomyces cerevisiae. This requires both the histidine kinase and the response response regulator domain of Hik1
Q9C1U1
into pQE30 and overexpressed in Escherichia coli M15
-
the sensing GAF domain of DosT is expressed in Escherichia coli BL21gold DE3 cells
O53473
expressed in Escherichia coli Tuner cells
-
expresssion of GST-tagged wild-type and mutant enzymes, quantitative expression analysis, overview
Q95434
wild-type and mutants introduced into deltarodK strain SA1708, His6-tagged wild-type and mutants expressed in Escherichia coli
-
expression of His-tagged 1-121 and truncated DELTA7 H-NOXA domains in Escherichia coli strain BL21(DE3)
-
isolation of cDNA; isolation of cDNA
Q9XH57, Q9XH58
no heterologous expression of NRII in Escherichia coli
P28788
Expression of wild-type and RoxRS mutant strain EU58 (mutant of two-component system) in Escherichia coli
Q88PG3
LOV-HK expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells; expression of His10-tagged full-length wild-type Ppr in Escherichia coli strain BL21(DE3), overexpression of the PYP domain of Ppr in Escherichia coli, co-expression of the full-length enzyme with tyrosine ammonia-lyase and p-coumaric acid ligase
-
cloned from a Salmonella typhi Ty2 cosmid bank and characterized by DNA sequence analysis
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
; cloned from a root cDNA library
O49187
expressed in Escherichia coli BL21(DE3) cells
Q9S1J9
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells; gene senS, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue
-
gene senS, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain XL-1 Blue
-
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli BL21(DE3) cells
-
overexpression in Escherichia coli, histidine kinase Hik34
-
expressed in Escherichia coli BL21 (DE3) CodonPlus RIL-X
Q9X180
expression of selenomethionine-labeled DctB residues 28-286 in Escherichia coli strain BL21(DE3)
-
cytoplasmic expression of TorSS as a fusion protein, containing a hexahistidine tag on the N-terminus separated by a separated by a thrombin protease recognition sequence, in Escherichia coli BL21(DE3) cells
-
downstream of a histidine tag into vector pET15b, His-tagged full-length RaxH and RaxR overexpressed in Escherichia coli BL21 cells in the presence of sorbitol and glycine betaine to produce soluble His-RaxH
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
during Anaplasma phagocytophilum development in human promyelocytic HL-60 cells, PleC is downregulated prior to extracellular release
Q2GJD7
during Anaplasma phagocytophilum development in human promyelocytic HL-60 cells, PleC is upregulated at the exponential growth stage
Q2GJD7
DesK is cold-activated
O34757
promoters of fphA, hk-8-2, and hk-8-5 preferentially function in conidial heads, the promoter of phkA preferentially functions in cleistothecia, and the promoters of tcsB and nikA function in both conidial heads and cleistothecia
-
promoters of fphA, hk-8-2, and hk-8-5 preferentially function in conidial heads, the promoter of phkA preferentially functions in cleistothecia, and the promoters of tcsB and nikA function in both conidial heads and cleistothecia
Emericella nidulans ABPU1
-
-
histidine kinase mRNA accumulation is induced by 140 mM acetosyringone
-
PhoQ is activated by acidic pH (pH 5.5)
-
Ppr is downregulated in the dark
-
red and white light upregulate the Ppr autokinase activity
-
PhoQ is activated by acidic pH (pH 5.5)
-
2.5fold enhanced transcription of SaeS after incubation with Perform and SDS
Q840P7
2.5fold enhanced transcription of SaeS after incubation with Perform and SDS
Staphylococcus aureus Newman
-
-
regulated by the small molecule autoinducer AI-2
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
S147L
Bacillus anthracis 34F2DELTA118
-
a BA2291 mutant, which mimics the behavior of the overexpressed wild-type protein.The S147L mutation does not affect the interaction of the kinase with Spo0F or the rate of phosphoryl group transfer to it
-
A413R
-
the mutation impairs KinA activity, folding or stability
Ala413L
-
the mutation impairs KinA activity, folding or stability
F432C
-
the substitution has less effect on Sda binding
F436S
-
the mutation increases the efficiency of sporulation in cells overexpressing KinA inhibitor Sda by about 10fold while actually reducing the efficiency of sporulation in cells that lack sda
H188V
O34757
the mutant retains the phosphatase activity of the wild type protein, the mutation triggers formation of an N-terminal 2-helical coiled-coil and extensive dimerization and histidine phosphotransfer domain-ATP-binding domain association
H430C
-
the substitution has less effect on Sda binding
I108A
P16497
the point mutation does not affect KinA autophosphorylation activity, but interfers with KinA PAS-A dimerization
I95A
P16497
the point mutation slightly reduces the KinA autophosphorylation activity and interfers with KinA PAS-A dimerization
P410L
-
the mutation increases the efficiency of sporulation in cells overexpressing KinA inhibitor Sda by about 10fold while actually reducing the efficiency of sporulation in cells that lack sda
P410L/F436S
-
the mutant has a low affinity for inhibitor Sda
Y29A
P16497
the point mutation is an activating mutation in full-length KinA, but interfers with KinA PAS-A dimerization shifting the PAS-A monomer/dimer equilibrium significantly toward the monomeric form
I108A
Bacillus subtilis 1A40
-
the point mutation does not affect KinA autophosphorylation activity, but interfers with KinA PAS-A dimerization
-
I95A
Bacillus subtilis 1A40
-
the point mutation slightly reduces the KinA autophosphorylation activity and interfers with KinA PAS-A dimerization
-
Y29A
Bacillus subtilis 1A40
-
the point mutation is an activating mutation in full-length KinA, but interfers with KinA PAS-A dimerization shifting the PAS-A monomer/dimer equilibrium significantly toward the monomeric form
-
D286C
-
t1/2 at room temperature is 16 min, compared to 12 min for wild-type enzyme. 71% of the wild-type phosphatase activity
E257C
-
t1/2 at room temperature is 30 min, compared to 12 min for wild-type enzyme. 31% of the wild-type phosphatase activity
E261C
-
t1/2 at room temperature is 15 min, compared to 12 min for wild-type enzyme. 77% of the wild-type phosphatase activity
E268C
-
t1/2 at room temperature is 23 min, compared to 12 min for wild-type enzyme. 45% of the wild-type phosphatase activity
E275C
-
t1/2 at room temperature is 28 min, compared to 12 min for wild-type enzyme. 34% of the wild-type phosphatase activity
E276C
-
t1/2 at room temperature is 68 min, compared to 12 min for wild-type enzyme. 5% of the wild-type phosphatase activity
E282C
-
t1/2 at room temperature is 15 min, compared to 12 min for wild-type enzyme. 77% of the wild-type phosphatase activity
G240C
-
t1/2 at room temperature is 10 min, compared to 12 min for wild-type enzyme. 123% of the wild-type phosphatase activity
G264C
-
t1/2 at room temperature is 12 min, compared to 12 min for wild-type enzyme. As active as wild-type enzyme
H243K
-
inactive mutant protein
H243N
-
inactive mutant protein
H243S
-
inactive mutant protein
H243V
-
inactive mutant protein
H243X
-
the His residue at position 243 of the EnvZ protein is changed by means of site-directed mutagenesis. The mutant EnvZ protein is defective in its in vitro ability not only as to EnvZ-autophosphorylation but also OmpR-phosphorylation and OmpR-dephosphorylation. This particular mutant EnvZ protein seems to exhibit null functions as to the in vivo osmoregulatory phenotype
H243Y
-
inactive mutant protein
H398L
-
the mutant retains itscapability to bind ATP and is competent to catalyze the transphosphorylation of an AtoS G-box (G565A) mutant protein which otherwise fails to autophosphorylate due to its inability to bind ATP
K272C
-
t1/2 at room temperature is 55 min, compared to 12 min for wild-type enzyme. 10% of the wild-type phosphatase activity
L236C
-
t1/2 at room temperature is 25 min, compared to 12 min for wild-type enzyme. 40% of the wild-type phosphatase activity
N248A
-
site-directed mutagenesis, the mutation activates the enzyme
N248D
-
site-directed mutagenesis, the mutation activates the enzyme
N248G
-
site-directed mutagenesis, the mutation activates the enzyme
N271C
-
t1/2 at room temperature is 36 min, compared to 12 min for wild-type enzyme. 23% of the wild-type phosphatase activity
N278C
-
t1/2 at room temperature is 45 min, compared to 12 min for wild-type enzyme. 15% of the wild-type phosphatase activity
Q262C
-
t1/2 at room temperature is 11 min, compared to 12 min for wild-type enzyme. 111% of the wild-type phosphatase activity
Q283C
-
t1/2 at room temperature is 13 min, compared to 12 min for wild-type enzyme. 91% of the wild-type phosphatase activity
R246C
-
t1/2 at room temperature is 62 min, compared to 12 min for wild-type enzyme. 7% of the wild-type phosphatase activity
S242C
-
t1/2 at room temperature is 39 min, compared to 12 min for wild-type enzyme. 20% of the wild-type phosphatase activity
S260C
-
t1/2 at room temperature is 13 min, compared to 12 min for wild-type enzyme. 91% of the wild-type phosphatase activity
S269C
-
t1/2 at room temperature is 20 min, compared to 12 min for wild-type enzyme. 54% of the wild-type phosphatase activity
T235C
-
t1/2 at room temperature is 14 min, compared to 12 min for wild-type enzyme. 84% of the wild-type phosphatase activity
T247R
-
inactive mutant protein
T250C
-
t1/2 at room temperature is 23 min, compared to 12 min for wild-type enzyme. 45% of the wild-type phosphatase activity
T256C
-
t1/2 at room temperature is 27 min, compared to 12 min for wild-type enzyme. 36% of the wild-type phosphatase activity
Y265C
-
t1/2 at room temperature is 20 min, compared to 12 min for wild-type enzyme. 54% of the wild-type phosphatase activity
H44A
-
the mutation may increase the side chain pKa of acidic amino acids in the input domain, whose protonation contributes to the activation of ArsS
H44A
-
the mutation may increase the side chain pKa of acidic amino acids in the input domain, whose protonation contributes to the activation of ArsS
-
H94A
-
H94 in the input domain of ArsS is crucial for acid perception
-
D56N
-
phosphorylation of CitB is inhibited by a D56N exchange. In the presence of ATP, CitB-D56N forms a stable complex with MalE-CitAC
H350L
-
autokinase activity of CitA is abolished by an H350L exchange
D657N
-
substitution in the first receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, rare sporulation outside fruiting bodies, but normal fruiting body morphology on CF agar, loose aggregates of fruiting bodies in submerged culutre
D657N/D782N
-
delayed timing of aggregation, frequent sporulation outside fruiting bodies, and loose mounds of fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, no autophosphorylation
D657N/D782N/D909N
-
delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates
D657N/D909N
-
delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular and small fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates
D696A
Q95434
site-directed mutagenesis, the mutation of the receiver domain residue leads to release of phosphate to the medium, overview
D782N
-
substitution in the second receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular and small fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre
D782N/D909N
-
delayed timing of aggregation, frequent sporulation outside fruiting bodies, and loose mounds of fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre, autophosphorylates
D909N
-
substitution in the third receiver domain, is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, but normal fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre
H360A
-
is unable to complement the deltarodK strain SA1708, delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre
A143G
-
the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations
D110A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
D131A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium; the purified mutant enzyme shows no fluorescence change well into the mM range of Mg2+, suggesting that this mutation renders the protein incapable of binding Mg2+
E115A
-
the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations
E133A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
E77A
-
the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations
G139A
-
the mutant still has some divalent cation-mediated repression and therefore likely has some ability to bind cations
L137A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
L158A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
Q188L
Q9HWR3
site-directed mutagenesis, crystal structure determination and analysis
S145A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
H146R
Q88KY2
the mutation does not affect the ability to sense copper
H146R/H147R/H149R
Q88KY2
the mutant shows minimal copper inducibility compared to wild type CinS
H147R
Q88KY2
the mutation has an almost 10fold reduced copper-dependent induction of cinAQ compared to the wild type
H147R/H149R
Q88KY2
the mutant shows minimal copper inducibility compared to wild type CinS
H149R
Q88KY2
the mutation does not affect the ability to sense copper
H37R
Q88KY2
the mutation has an almost 10fold reduced copper-dependent induction of cinAQ compared to the wild type
A143G
-
the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations
D110A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
D131A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium; the purified mutant enzyme shows no fluorescence change well into the mM range of Mg2+, suggesting that this mutation renders the protein incapable of binding Mg2+
E115A
-
the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations
E133A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
E77A
-
the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations
G139A
-
the mutant still has some divalent cation-mediated repression and therefore likely have some ability to bind cations
L137A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
L158A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
S145A
-
the mutant exhibits strong de-repression in medium containing 10 mM Mg2+, and activity is approximately the same regardless of the cation content in the growth medium
RD51A
-
lacks phosphorylation activities
RD51A
Xanthomonas oryzae pv. oryzae PXO99
-
lacks phosphorylation activities
-
H405A
-
negative mutation of CKI1
additional information
-
construction of ahk5 T-DNA insertion mutants, Arabidopsis mutants lacking AHK5 show reduced stomatal closure in response to H2O2, which is reversed by complementation with the wild type gene. Overexpression of AHK5 results in constitutively less stomatal closure. Abiotic stimuli that generate endogenous H2O2, such as darkness, nitric oxide and the phytohormone ethylene, also show reduced stomatal closure in the ahk5 mutants. However, ABA causes closure, dark adaptation induces H2O2 production and H2O2 induces NO synthesis in mutants, phenotype, overview
additional information
-, Q81QX4
the BA2291 gene is capable of complementing Bacillus subtilis sporulation kinase-deficient mutants when present in the cell in single copy, and its deletion in Bacillus anthracis strongly reduces the ability of the organism to sporulate. BA2291 overexpression in Bacillus subtilis completely prevents sporulation, also in a wild type strain
S147L
-, Q81QX4
a BA2291 mutant, which mimics the behavior of the overexpressed wild-type protein.The S147L mutation does not affect the interaction of the kinase with Spo0F or the rate of phosphoryl group transfer to it
additional information
Bacillus anthracis 34F2DELTA118
-
the BA2291 gene is capable of complementing Bacillus subtilis sporulation kinase-deficient mutants when present in the cell in single copy, and its deletion in Bacillus anthracis strongly reduces the ability of the organism to sporulate. BA2291 overexpression in Bacillus subtilis completely prevents sporulation, also in a wild type strain
-
I95E
P16497
the point mutation reduces the KinA autophosphorylation activity and interfers with KinA PAS-A dimerization
additional information
P16497
a significant decrease occurs of the autophosphorylation rate of a KinA protein lacking the N-terminal Per-ARNT-Sim domain, which plays a critical role in the catalytic activity of this enzyme
additional information
Q45614
YycG depletion does not interfere with FtsZ localization, YycG is less active as a kinase of YycF in the absence of a division septum
I95E
Bacillus subtilis 1A40
-
the point mutation reduces the KinA autophosphorylation activity and interfers with KinA PAS-A dimerization
-
additional information
Bacillus subtilis 1A40
-
a significant decrease occurs of the autophosphorylation rate of a KinA protein lacking the N-terminal Per-ARNT-Sim domain, which plays a critical role in the catalytic activity of this enzyme
-
H1172Q
-
mutation abolishes BvgS activity in vivo and eliminates detectable phosphorylation of BvgA in vitro. Activity of BvgS H1172Q can be restored by providing the wild-type C-terminal domain in trans
additional information
-
bos1-null mutants, osmosensitivity and resistance to fungicides, very rarely form conidiophores and those few conidiophores fail to produce conidia, this defect can be partially restored with 1000 mM sorbitol. Mutants are not hypersensitive to various oxidative stresses but are more resistant to menadione, have significantly reduced ability to infect host plants. Appressorium morphogenesis is not altered, however, in planta growth is severely reduced
additional information
Botryotinia fuckeliana UWS111
-
bos1-null mutants, osmosensitivity and resistance to fungicides, very rarely form conidiophores and those few conidiophores fail to produce conidia, this defect can be partially restored with 1000 mM sorbitol. Mutants are not hypersensitive to various oxidative stresses but are more resistant to menadione, have significantly reduced ability to infect host plants. Appressorium morphogenesis is not altered, however, in planta growth is severely reduced
-
C69A
-
mutant in LOV-HK knockout background does not return the replication rate to the wild-type level
additional information
Q57BR6
deletion of the pdhS in the presence of a rescue copy expressed from pRH232, pRH232 is replaced in the deltapdhS strain only in the presence of another wild-type copy of pdhS through pRH404
additional information
-
insertion-knockout mutant strain by introduction of a kanamycin-resistant cassette into the gene, replication is less than the wild-type strain as early as 4 hours after infection with J774A.1 murine macrophages
additional information
Brucella abortus XDB1104
-
deletion of the pdhS in the presence of a rescue copy expressed from pRH232, pRH232 is replaced in the deltapdhS strain only in the presence of another wild-type copy of pdhS through pRH404
-
C69A
-
LOV-HK mutant, binds flavin mononucleotide but cannot form the cysteinyl adduct, does not activate the kinase in response to illumination
additional information
-
the disruption of a PAS-like motif in the sensor domain affects the stability of CckA accumulation at the poles accompanied by a partial loss in CckA function. Shortening of an extended linker between beta-sheets within the CckA catalysis-assisting ATP-binding domain leads to a dramatic cell-to-cell variability in CpdR levels and CtrA cell cycle periodicity
additional information
-
in the deltamtrAB mutant, the expression of carrier genes is abolished (betP) or markedly reduced, MtrB mutants in which either the large periplasmic loop or the HAMP domain is deleted, are regulated similar to wild-type MtrB
additional information
-
ethanolamine utilization and growth on it is abolished in the response regulator RR17 mutant strain lacking a functional partner of histidine kinase in the RR-HK17 two-component system, phenotype, overview
L254C
-
inactive mutant enzyme, t1/2 at room temperature is 90 min, compared to 12 min for wild-type enzyme
additional information
-
cheA mutations leading to defects in chemotaxis are mapped and characterized
additional information
-
construction of several phoR genes, with various deletions in the 5' regions, which are regulated by the trp-lac hybrid promoter. The PhoR1084 and PhoR1159 proteins that lack the 83 and 158 N-terminal amino acids, respectively, retain the positive function for the expression of phoA that codes for alkaline phosphatase, but lack the negative function. The PhoR1263 protein that lacks the 262 N-terminal amino acids is deficient in both functions
additional information
-
the histidine phosphorylation sites of each TorS transmitter domain and the aspartate phosphorylation site of the TorS receiver are individually changed by site-directed mutagenesis. All three phosphorylation sites proved essential for in vivo induction of the tor structural operon and for in vitro transphosphorylation of the cognate TorR response regulator. The His to Gln change in the classical transmitter domain abolished TorS autophosphorylation, whereas TorS undergoes significant autophosphorylation when the phosphorylation site of its receiver or alternative transmitter is changed
additional information
-
mutations within the linker HAMP domain block the osmosensing function of EnvZ
H94A
-
H94 in the input domain of ArsS is crucial for acid perception
additional information
-
growth of HP0244 mutant strain is similar to the growth of the wild-type strain, null mutations in either HP0165 or HP1364 histidine kinases are impaired in their ability to grow at pH 5.0, acid-responsive gene transcription is altered in the HP0165 and HP1364 null mutant strains compared to the parental wild-type strain, growth of complemented mutant strains is similar to that of the wild-type strain
additional information
Q25026
an acid survival study using an HP0703 mutant and an electrophoretic mobility shift assay with in vitro-phosphorylated HP0703 showe, that HP0703 does not contribute to acid survival and does not bind to the promoter regions of several genes in the HP0244 pH-dependent regulon, suggesting that there is a pathway outside the HP0703 regulon which transduces the acid-responsive signal sensed by HP0244
H407A
Q95434
site-directed mutagenesis, the mutation of the autophosphorylation site residue H407 inhibits autophosphorylation of the enzyme
additional information
-
delayed timing of aggregation, frequent sporulation outside fruiting bodies, and irregular fruiting body morphology on CF agar, no aggregation of fruiting bodies in submerged culutre
additional information
Q95434
construction of several mutants by in-frame deletions, espA mutants, encoding an orphan hybrid histidine kinase, alter the timing of the organism's developmental program, greatly accelerating developmental progression, phenotype, overview
additional information
-
deletion of 7 N-terminal enzyme residues affects dimer organization
G394A/G396A
-
completely inactive
additional information
Q840P7
a point mutation in SaeS of strain Newman is responsible for increased expression of Eap upon exposure to sublethal Perform and SDS concentrations, leading to increased extracellular adherence protein-dependent cellular invasiveness
additional information
Staphylococcus aureus Newman
-
a point mutation in SaeS of strain Newman is responsible for increased expression of Eap upon exposure to sublethal Perform and SDS concentrations, leading to increased extracellular adherence protein-dependent cellular invasiveness
-
additional information
-
construction of a senS disruption and a truncated mutant
additional information
Streptomyces reticuli Tue54
-
construction of a senS disruption and a truncated mutant; construction of a senS disruption and a truncated mutant
-
additional information
-
inactivation of each putative hik gene, no significant alterations in gene expression in most of the mutants. DeltaHik33 and deltaHik19 exhibit reduced ability to activate luciferase at low temperature. Deltahik27, deltahik34, and deltahik20 show enhanced expression of some genes, whereas others are repressed. In deltaHik34 cells, levels of transcripts of heat-shock genes are elevated. In deltaHik33-, deltaHik34-, deltaHik16-, and deltaHik41-mutant cells gene expression is significantly affected by elevated levels of NaCl
additional information
-
mutation of hik41 does not affect the expression of the genes located downstream of this gene, 22 H2O2-inducible genes are either totally or almost totally unresponsive to H2O2 in deltaHik33 mutant cells, whereas mutation of Hik34, Hik16 or Hik41 abolishes the H2O2 induction of only two genes, Hik16 and Hik41 regulate the H2O2-inducible expression of the same two genes
additional information
Q9X180
delta408ThkA, includes the PAS-sensor, the DHp and CA domains, and delta 517ThkA without the PAS-sensor domain, both are fully active in the autophosphorylation reaction
H222A
-
lacks phosphorylation activities
additional information
-
truncated His-RaxH, lacking the N-terminal transmembrane region, shows similar autophosphorylation activities than His-tagged full-length RaxH, but is unable to induce phosphorylation of His-RaxR
H222A
Xanthomonas oryzae pv. oryzae PXO99
-
lacks phosphorylation activities
-
additional information
Xanthomonas oryzae pv. oryzae PXO99
-
truncated His-RaxH, lacking the N-terminal transmembrane region, shows similar autophosphorylation activities than His-tagged full-length RaxH, but is unable to induce phosphorylation of His-RaxR
-
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
protein refolding is achieved by dialysis in storage buffer (50 mM Tris-HCl pH 7.6, 0.5 mM dithiothreitol, 50% (v/v) glycerol) containing 0.01% (v/v) Triton X-100
-
chemical reconstitution of the recombinantly expressed PYP domain of Ppr with 4-coumaric acid
-
the refolded recombinant protein is obtained by gradual removal of 6 M urea by sequential dialysis
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
BOS1 is necessary for normal osmoregulation, is not the general oxidative stress signal transducer, is required for normal macroconidiation and full virulence
additional information
Botryotinia fuckeliana UWS111
-
BOS1 is necessary for normal osmoregulation, is not the general oxidative stress signal transducer, is required for normal macroconidiation and full virulence
-
additional information
Q57BR6
PdhS contains an N-terminal multimerization part and a C-terminal histidine kinase part interacting with DivK, PdhS can enhance the phosphorylation of DivK and its subsequent polar localization, the enzyme has essential functions in controlling some aspects of the division process, maybe through the control of CtrA activity, PdhS recognizes a polar structure conserved in several alpha-proteobacteria
additional information
-
a LOV domain at the N-terminus, followed by a PAS domain in the intervening sequence, and a histidine kinase at the C-terminus
additional information
Brucella abortus XDB1104
-
PdhS contains an N-terminal multimerization part and a C-terminal histidine kinase part interacting with DivK, PdhS can enhance the phosphorylation of DivK and its subsequent polar localization, the enzyme has essential functions in controlling some aspects of the division process, maybe through the control of CtrA activity, PdhS recognizes a polar structure conserved in several alpha-proteobacteria
-
additional information
-
a LOV domain at the N-terminus, followed by a PAS domain in the intervening sequence, and a histidine kinase at the C-terminus
additional information
-
MtrB belongs to a class of histidine protein kinases that sense environmental changes at cytoplasmatic protein domains independently of the periplasmic loop and the cytoplasmic HAMP domain
additional information
-
LOV domain followed by a linking sequence and a C-terminal histidine kinase domain
additional information
-
15 of the 30 known Escherichia coli histidine kinases contain a single HAMP domain, has an important role in signal transduction
additional information
-
intact HP0165 and HP1364 histidine kinases are required for acid resistance in Helicobacter pylori
additional information
-
great effect of temperature on the dynamics of the HAMP linker domain, which is a key feature in signal transduction
additional information
-
hybrid histidine protein kinase in which four signalling domains are required for full activity
additional information
-
LOV domain at the N terminus, followed by a histidine kinase and a putative C-terminal receiver domain
additional information
Pseudomonas syringae DC3000
-
LOV domain at the N terminus, followed by a histidine kinase and a putative C-terminal receiver domain
-
medicine
-
significance in pathological virulence of Salmonella enterica, seductive target for anti-bacterial therapeutic development
additional information
-
44 genes for Hiks on the chromosome, Hik34 might act as a negative regulator of the expression of heat-shock genes during normal growth. Hik33 is a cold sensor, participates in the positive regulation of the expression of more than 60% of the cold-inducible genes, is also involved in the perception of hyperosmotic stress and salt stress and transduction of the signals, findings cannot be explained by the current model of two-component systems
additional information
-
Hik33, Hik34, Hik16 and Hik41, are involved in perception and transduction of H2O2 signals that regulate the gene expression of 26 of the 77 H2O2-inducible genes with induction factors higher than 4.0, Hik33 is the main contributor
additional information
Q9X180
responsible role of the response regulator TrrA for the feedback regulation of sensing and/or kinase activities of ThkA
additional information
-
induces phosphorylation of its concomitant response regulator RaxR
additional information
Xanthomonas oryzae pv. oryzae PXO99
-
induces phosphorylation of its concomitant response regulator RaxR
-