Cloned (Comment) | Organism |
---|---|
overexpression of nonreceptor-type HK gene cytokinin independent1 (CKI1) activates cytokinin signaling, quantitative RT-PCR expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the T-DNA insertion is transmitted through female gametophytes at an extremely low frequency in CKI1/cki1-8 plants, while cki1-6 mutation, which carries a T-DNA at a more distal location in the CKI1 promoter than cki1-8 mutatin does not appear to be capable of being transmitted through female gametophytes. The cki1-8 mutation impairs female gametophyte development, cki1-8 mutant phenotype during reproductive development and vegetative growth. Mutant phenotypes, overview | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | CKI1 interacts with proteins AHP2 and AHP3, but CKI1 is incapable of binding to cytokinins | ? | - |
? | |
additional information | Arabidopsis thaliana Col-0 | CKI1 interacts with proteins AHP2 and AHP3, but CKI1 is incapable of binding to cytokinins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O22267 | - |
- |
Arabidopsis thaliana Col-0 | O22267 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
female gametophyte | - |
Arabidopsis thaliana | - |
flower | - |
Arabidopsis thaliana | - |
flower bud | - |
Arabidopsis thaliana | - |
additional information | no expression of CKI1 in seedling, roots, stem, and leaves | Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CKI1 interacts with proteins AHP2 and AHP3, but CKI1 is incapable of binding to cytokinins | Arabidopsis thaliana | ? | - |
? | |
additional information | CKI1 interacts with proteins AHP2 and AHP3, but CKI1 is incapable of binding to cytokinins | Arabidopsis thaliana Col-0 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
At2g47430 | - |
Arabidopsis thaliana |
CKI1 | - |
Arabidopsis thaliana |
cytokinin independent1 | - |
Arabidopsis thaliana |
histidine kinase CKI1 | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | the female gametophytic lethal phenotype of cki1-5 (a null mutant) can be partially rescued by IPT8 or ARR1 (a type-B Arabidopsis RR) driven by a CKI1 promoter. A mutant allele, cki1-8, is cki1-8 is a weak mutant allele with residual CKI1 expression, and can be transmitted through female gametophytes with low frequency, viable homozygous cki1-8 mutant plants that grow larger than wild-type plants, show defective megagametogenesis, and rarely set enlarged seeds. The cki1-8 mutation impairs female gametophyte development, phenotype overview. Mutant cki1-8 responds normally to cytokinin, remarkably smaller siliques are formed in cki1-8 plants than in the wild type | Arabidopsis thaliana |
metabolism | gene CKI1 is part of the genetic pathway consisting of CKI1, AHPs, and type-B ARRs in the regulation of female gametophyte development and vegetative growth | Arabidopsis thaliana |
physiological function | cytokinin signaling is mediated by a multiple-step phosphorelay. Key components of the phosphorelay consist of the histidine kinase (HK)-type receptors, histidine phosphotransfer proteins (HP), and response regulators (RRs). Histidine kinase CKI1 acts upstream of histidine phosphotransfer proteins to regulate female gametophyte development and vegetative growth, role of AHP proteins in female gametophyte development, overview | Arabidopsis thaliana |