Cloned (Comment) | Organism |
---|---|
gene ahk5, transient expression of GST-tagged full-length AHK5 in Arabidopsis thaliana protoplasts and Nicotiana benthamiana leaf cells the fusion protein in the cytoplasm, independent of whether the GFP tag is fused to the N-terminus or C-terminus of AHK5, expression profile of AHK5 in different tissues and cell types by semi-quantitative RT-PCR | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of ahk5 T-DNA insertion mutants, Arabidopsis mutants lacking AHK5 show reduced stomatal closure in response to H2O2, which is reversed by complementation with the wild type gene. Overexpression of AHK5 results in constitutively less stomatal closure. Abiotic stimuli that generate endogenous H2O2, such as darkness, nitric oxide and the phytohormone ethylene, also show reduced stomatal closure in the ahk5 mutants. However, ABA causes closure, dark adaptation induces H2O2 production and H2O2 induces NO synthesis in mutants, phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Arabidopsis thaliana | 5737 | - |
cytosol | - |
Arabidopsis thaliana | 5829 | - |
plasma membrane | - |
Arabidopsis thaliana | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | histidine kinases are part of two-component signal transduction systems that act to integrate environmental stimuli into a cellular response via a phosphotransfer relay mechanism, e.g. involved in stomatal guard cell response to H2O2, regulation of the physiological function, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | the enzyme performs regulatory autophosphorylation | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
guard cell | - |
Arabidopsis thaliana | - |
guard cell | expressed at low levels in guard cells | Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | histidine kinases are part of two-component signal transduction systems that act to integrate environmental stimuli into a cellular response via a phosphotransfer relay mechanism, e.g. involved in stomatal guard cell response to H2O2, regulation of the physiological function, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | the enzyme performs regulatory autophosphorylation | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AHK5 | - |
Arabidopsis thaliana |
hK5 | - |
Arabidopsis thaliana |
More | AHK5 belongs to the the canonical HK class of proteins in Arabidopsis thaliana | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | Arabidopsis mutants lacking HK5 show reduced stomatal closure in response to H2O2, overexpression of HK5 results in constitutively less stomatal closure | Arabidopsis thaliana |
physiological function | functional HK5 histidine kinase is required for H2O2 responses in stomatal guard cells | Arabidopsis thaliana |