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Literature summary for 2.7.13.3 extracted from

  • Kim, T.; Choi, J.; Lee, S.; Yeo, K.J.; Cheong, H.K.; Kim, K.K.
    Structural studies on the extracellular domain of sensor histidine kinase YycG from Staphylococcus aureus and its functional implications (2016), J. Mol. Biol., 428, 3074-3089.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene yycG, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3), the SeMet-substituted YycG is produced in Escherichia coli methionine auxotrophic strain B834(DE3) Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
L128C site-directed mutagenesis Staphylococcus aureus
additional information cross-linking of the recombinant the SeMet-substituted YycG enzyme Staphylococcus aureus
N44C/G142C site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased Staphylococcus aureus
N44C/G143C site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased Staphylococcus aureus
N44C/R146C site-directed mutagenesis, introducing cysteine substitution at Arg146 of YycG N44C mutant does not show any high molecular weight bands, but it shows only the dimeric band of YycG N44C Staphylococcus aureus
S81C site-directed mutagenesis Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
additional information YycH and YycI are two auxiliary membrane proteins that inhibit the activity of YycG HK in Bacillus subtilis. Both YycH and YycI are required to inhibit the dimerization of YycG Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
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differs from strain NCTC8325 and contains a number of genetic polymorphisms
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Staphylococcus aureus Q2G2U4
-
-
Staphylococcus aureus NCTC 8325 Q2G2U4
-
-
Staphylococcus aureus RN4220
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differs from strain NCTC8325 and contains a number of genetic polymorphisms
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and dialysis. His-tag removal by tobacco etch virus protease is followed by nickel affinity chromatography and gel filtration Staphylococcus aureus

Subunits

Subunits Comment Organism
dimer the extracellular domain of enzyme YycG exhibits two dimerization modes, dimerization configurations, cross-linking experiments, overview. Both YycH and YycI are required to inhibit the dimerization of YycG Staphylococcus aureus
More Both YycH and YycI are required to inhibit the dimerization of YycG. Genes yycH and yycI are members of the yyc operon. The actions of YycH and YycI on YycG likely occur at the transmembrane helices, in which an octameric complex was formed by an YycG homodimer flanked by YycH-YycI heterodimers Staphylococcus aureus
oligomer intact YycG HK can form higher-order oligomers at the cell membrane via combinations of the H- and L-dimeric interacting surfaces Staphylococcus aureus

Synonyms

Synonyms Comment Organism
SAOUHSC_00021
-
Staphylococcus aureus
sensor histidine kinase
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Staphylococcus aureus
YycG
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Staphylococcus aureus