Cloned (Comment) | Organism |
---|---|
gene yycG, recombinant expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21(DE3), the SeMet-substituted YycG is produced in Escherichia coli methionine auxotrophic strain B834(DE3) | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
L128C | site-directed mutagenesis | Staphylococcus aureus |
additional information | cross-linking of the recombinant the SeMet-substituted YycG enzyme | Staphylococcus aureus |
N44C/G142C | site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased | Staphylococcus aureus |
N44C/G143C | site-directed mutagenesis, the monomeric band intensities of the double mutant are dramatically decreased | Staphylococcus aureus |
N44C/R146C | site-directed mutagenesis, introducing cysteine substitution at Arg146 of YycG N44C mutant does not show any high molecular weight bands, but it shows only the dimeric band of YycG N44C | Staphylococcus aureus |
S81C | site-directed mutagenesis | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | YycH and YycI are two auxiliary membrane proteins that inhibit the activity of YycG HK in Bacillus subtilis. Both YycH and YycI are required to inhibit the dimerization of YycG | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
differs from strain NCTC8325 and contains a number of genetic polymorphisms | - |
Staphylococcus aureus | Q2G2U4 | - |
- |
Staphylococcus aureus NCTC 8325 | Q2G2U4 | - |
- |
Staphylococcus aureus RN4220 | - |
differs from strain NCTC8325 and contains a number of genetic polymorphisms | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and dialysis. His-tag removal by tobacco etch virus protease is followed by nickel affinity chromatography and gel filtration | Staphylococcus aureus |
Subunits | Comment | Organism |
---|---|---|
dimer | the extracellular domain of enzyme YycG exhibits two dimerization modes, dimerization configurations, cross-linking experiments, overview. Both YycH and YycI are required to inhibit the dimerization of YycG | Staphylococcus aureus |
More | Both YycH and YycI are required to inhibit the dimerization of YycG. Genes yycH and yycI are members of the yyc operon. The actions of YycH and YycI on YycG likely occur at the transmembrane helices, in which an octameric complex was formed by an YycG homodimer flanked by YycH-YycI heterodimers | Staphylococcus aureus |
oligomer | intact YycG HK can form higher-order oligomers at the cell membrane via combinations of the H- and L-dimeric interacting surfaces | Staphylococcus aureus |
Synonyms | Comment | Organism |
---|---|---|
SAOUHSC_00021 | - |
Staphylococcus aureus |
sensor histidine kinase | - |
Staphylococcus aureus |
YycG | - |
Staphylococcus aureus |