Literature summary for 2.7.13.3 extracted from

Cheung, J.; Hendrickson, W.A.
Structural analysis of ligand stimulation of the histidine kinase NarX (2009), Structure, 17, 190-201.

Search for more literature for 2.7.13.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
free and in complex with nitrate, hanging drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5) and 2 M NH4H2PO4 at 4°C (enzyme in complex with nitrate) or using 23% (w/v) polyethylene glycol 3350, 1% (v/v) isopropanol, and 0.1 M HEPES (pH 7.5) at 4°C (free apo-enzyme)	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0AFA2	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-affinity column chromatography, MonoQ column chromatography, glutathione Sepharose column chromatography, Superdex 200 gel filtration, and Superdex 75 gel filtration	Escherichia coli

Subunits

Subunits	Comment	Organism
monomer	gel filtration	Escherichia coli

Synonyms

Synonyms	Comment	Organism
NarX	-	Escherichia coli
nitrate-responsive histidine kinase	-	Escherichia coli

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Release 2023.1 (January 2023)