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Literature summary for 2.7.13.3 extracted from

  • Ibrahim, I.M.; Puthiyaveetil, S.; Allen, J.F.
    A two-component regulatory system in transcriptional control of photosystem stoichiometry: redox-dependent and sodium ion-dependent phosphoryl transfer from cyanobacterial histidine kinase Hik2 to response regulators Rre1 and RppA (2016), Front. Plant Sci., 7, 137.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene slr1147, recombinant expression of C-terminally poly-His-tagged full-length enzyme in Escherichia coli strain BL21(DE3) Synechocystis sp. PCC 6803

Inhibitors

Inhibitors Comment Organism Structure
additional information upon salt and/or hyperosmotic signal, the activity of Hik2 is inhibited. KCl does not inhibit the autophosphorylation activity of Hik2 Synechocystis sp. PCC 6803
Na+ strongly inhibits the autophosphorylation activity of Hik2. 0.5 M NaCl reduces the organism's growth rate by 50% Synechocystis sp. PCC 6803

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Synechocystis sp. PCC 6803 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protein L-histidine Synechocystis sp. PCC 6803
-
ADP + protein N-phospho-L-histidine
-
?
additional information Synechocystis sp. PCC 6803 interaction of Hik2 with Rre1 and RppA, usage of recombinant N-terminally MBP-tagged proteins purified from Escherichia coli expression host ?
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803 P73276
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein Hik2 undergoes autophosphorylation on a conserved histidine residue, at pH 7.5 and 22°C, and becomes rapidly dephosphorylated by the action of response regulators Rre1 and RppA (regulator of phycobilisome association), at pH 7.5 and 30°C. The autophosphorylation of Hik2 is specifically inhibited by sodium ions Synechocystis sp. PCC 6803

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally poly-His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration. Overexpression of te GST-tagged enzyme in a bait and prey two hybrid system in Saccharomyces cerevisiae Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein L-histidine
-
Synechocystis sp. PCC 6803 ADP + protein N-phospho-L-histidine
-
?
additional information interaction of Hik2 with Rre1 and RppA, usage of recombinant N-terminally MBP-tagged proteins purified from Escherichia coli expression host Synechocystis sp. PCC 6803 ?
-
?
additional information during autophosphorylation, histidine kinases catalyze transfer of only the gamma-phosphate from an ATP molecule to their conserved histidine residue Synechocystis sp. PCC 6803 ?
-
?

Synonyms

Synonyms Comment Organism
Hik2
-
Synechocystis sp. PCC 6803
histidine kinase
-
Synechocystis sp. PCC 6803
sensory transduction histidine kinase UniProt Synechocystis sp. PCC 6803
slr1147
-
Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
ATP the ATP-binding domain of histidine kinases contains conserved motifs essential for autokinase activity. These include G1 and G2 boxes, which have the characteristic glycine signatures DxGxG and GxGxG, respectively Synechocystis sp. PCC 6803

General Information

General Information Comment Organism
metabolism proposed signal transduction pathway for the Hik2-based two-component system in cyanobacteria, overview Synechocystis sp. PCC 6803
physiological function Hik2 is the cytoplasmic homologue of chloroplast sensor kinase (CSK), a protein involved in redox regulation of chloroplast gene expression during changes in light quality in plants and algae. Hik2 is the sensor that acts on Rre1 and RppA transcription factors to regulate photosynthesis genes as part of the mechanism of photosystem stoichiometry adjustment in cyanobacteria. Signaling mechanism of Hik2 and its phosphotransferase activity, overview. Hik2-based signaling pathway integrates acclimatory responses to light and salt stresses in cyanobacteria Synechocystis sp. PCC 6803