Literature summary for 2.7.13.3 extracted from

Ma, X.; Sayed, N.; Baskaran, P.; Beuve, A.; van den Akker, F.
PAS-mediated dimerization of soluble guanylyl cyclase revealed by signal transduction histidine kinase domain crystal structure (2008), J. Biol. Chem., 283, 1167-1178.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of His-tagged 1-121 and truncated DELTA7 H-NOXA domains in Escherichia coli strain BL21(DE3)	Nostoc punctiforme

Crystallization (Commentary)

Crystallization (Comment)	Organism
dimerized signal transduction histidine kinase domain H-NOXA domain residues 8-121, 4°C, sitting-drop vapor diffusion, 20 mg/ml NpSTHK8121 in 5 mM Tris, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, is mixed with an equal volume of reservoir solution containing 1.7-1.9 M ammonium sulfate, 100 mM Tris-HCl, pH 7.7-9.0, for crystallization of the full-length enzyme, 15 mg/ml protein is mixed with equal volume of 0.1 M HEPES, pH 7.5, and 1.5 M lithium sulfate monohydrate, crystals appear after 3 days at 20°C, X-ray diffraction structure determination and analysis at 2.1 A resolution	Nostoc punctiforme

Crystallization (Comment)

Organism

dimerized signal transduction histidine kinase domain H-NOXA domain residues 8-121, 4°C, sitting-drop vapor diffusion, 20 mg/ml NpSTHK8121 in 5 mM Tris, pH 7.5, 100 mM NaCl, 1 mM 2-mercaptoethanol, is mixed with an equal volume of reservoir solution containing 1.7-1.9 M ammonium sulfate, 100 mM Tris-HCl, pH 7.7-9.0, for crystallization of the full-length enzyme, 15 mg/ml protein is mixed with equal volume of 0.1 M HEPES, pH 7.5, and 1.5 M lithium sulfate monohydrate, crystals appear after 3 days at 20°C, X-ray diffraction structure determination and analysis at 2.1 A resolution

Nostoc punctiforme

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of 7 N-terminal enzyme residues affects dimer organization	Nostoc punctiforme

Organism

Organism	UniProt	Comment	Textmining
Nostoc punctiforme	-	strain PCC 73102	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged 1-121 and truncated DELTA7 H-NOXA domains from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Nostoc punctiforme

Subunits

Subunits	Comment	Organism
dimer	the signal transduction histidine kinase domain, H-NOXA revealing a Per-Arnt-Sim fold, monomers dimerize in a parallel arrangement juxtaposing their N-terminal helices and preceding residues, overview	Nostoc punctiforme
More	the N-terminal domain in an STHK from Nostoc punctiforme is homologous to the central region in soluble guanylyl cyclase, the main receptor for nitric oxide, structure-function analysis and dimerization mechanism, overview	Nostoc punctiforme

Synonyms

Synonyms	Comment	Organism
signal transduction histidine kinase	-	Nostoc punctiforme
StHK	-	Nostoc punctiforme