Literature summary for 2.7.13.3 extracted from

Fukushima, T.; Furihata, I.; Emmins, R.; Daniel, R.A.; Hoch, J.A.; Szurmant, H.
A role for the essential YycG sensor histidine kinase in sensing cell division (2011), Mol. Microbiol., 79, 503-522.

Cloned(Commentary)

Cloned (Comment)	Organism
gene yycG, recombinant expression of trncated enzyme mutants. Expression of a soluble cytoplasmic YycG construct in a bacterial two-hybrid expression system using the full-length YycG or a truncated YycGDELTA211-611 that lacks all cytoplasmic domains, and cell division proteins FtsZ, FtsA, FtsW, DivIB, DivIC, FtsL, Pbp2B, EzrA, ZapA, SepF and MinJ as C-terminal fusions to the individual domains (T18 and T25) of the two-domain Bordatella pertussis adenylate cyclase protein. YycG makes specific interactions with FtsL, DivIB, Pbp2B and interactions with FtsW and EzrA appear also possible	Bacillus subtilis

Cloned (Comment)

Organism

gene yycG, recombinant expression of trncated enzyme mutants. Expression of a soluble cytoplasmic YycG construct in a bacterial two-hybrid expression system using the full-length YycG or a truncated YycGDELTA211-611 that lacks all cytoplasmic domains, and cell division proteins FtsZ, FtsA, FtsW, DivIB, DivIC, FtsL, Pbp2B, EzrA, ZapA, SepF and MinJ as C-terminal fusions to the individual domains (T18 and T25) of the two-domain Bordatella pertussis adenylate cyclase protein. YycG makes specific interactions with FtsL, DivIB, Pbp2B and interactions with FtsW and EzrA appear also possible

Bacillus subtilis

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of several truncation mutant of enzyme YycG. All truncated YycG constructs including the shortest one that featured only the cytoplasmic PAS and the two catalytic HisKA and HATPase domains retain the ability to localize to the septum. N-terminal truncations of YycG lose negative regulation of their activity, truncated YycG constructs fail to co-immunoprecipitate with the regulatory proteins YycH and YycI	Bacillus subtilis

Inhibitors

Inhibitors	Comment	Organism	Structure
YycH	an extracytoplasmic membrane anchored protein. Enzyme YycG activity in non-dividing cells is suppressed by its interaction with YycH and YycI. YycG regulation is accomplished through its transmembrane and extra-membrane domains interacting with the membrane associated YycH and YycI proteins that do not localize to the divisome	Bacillus subtilis
YycI	an extracytoplasmic membrane anchored protein. Enzyme YycG activity in non-dividing cells is suppressed by its interaction with YycH and YycI. YycG regulation is accomplished through its transmembrane and extra-membrane domains interacting with the membrane associated YycH and YycI proteins that do not localize to the divisome	Bacillus subtilis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell septum	division septum, a cytoplasmic domain of YycG is sufficient for septum localization. All recombinant truncated YycG constructs including the shortest one that featured only the cytoplasmic PAS and the two catalytic HisKA and HATPase domains retain the ability to localize to the septum	Bacillus subtilis	30428	-
membrane	the YycG kinase is associated in the membrane as a complex with YycH and YycI through interaction of their transmembrane domains	Bacillus subtilis	16020	-

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	Q45614	-	-
Bacillus subtilis 168	Q45614	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	YycG interacts with the latter stage cell division proteins DivIB, Pbp2B and FtsL	Bacillus subtilis	?	-	?
additional information	YycG interacts with the latter stage cell division proteins DivIB, Pbp2B and FtsL	Bacillus subtilis 168	?	-	?

Synonyms

Synonyms	Comment	Organism
BSU40400	-	Bacillus subtilis
sensor histidine kinase	-	Bacillus subtilis
WalK	-	Bacillus subtilis
YycG	-	Bacillus subtilis
YycG kinase	-	Bacillus subtilis

General Information

General Information	Comment	Organism
malfunction	N-terminal truncations of YycG lose negative regulation of their activity, phenotypes, overview. Truncated YycG constructs fail to co-immunoprecipitate with the regulatory proteins YycH and YycI. Deletion or depletion of later stage cell division proteins does not perturb YycG localization	Bacillus subtilis
physiological function	The enzyme YycG is part of the two-component signal transduction system YycFG or WalRK. The YycG (WalK) sensor histidine kinase coordinates cell wall remodeling with cell division in Gram-positive bacteria by controlling the transcription of genes for autolysins and their inhibitors. The essential enzyme YycG senses cell division and is enzymatically activated by associating with the divisome at the division septum. The cytoplasmic PAS domain of this multidomain trans-membrane kinase is a determining factor translocating the kinase to the division septum. YycG activity in non-dividing cells is suppressed by its interaction with YycH and YycI and its activation is coordinated to cell division in dividing cells by specific interactions that occur within the divisome. This regulation is accomplished through its transmembrane and extra-membrane domains interacting with the membrane associated YycH and YycI proteins that do not localize to the divisome. Signaling by YycG involves later stage cell division proteins, overview	Bacillus subtilis