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7.1.2.2: H+-transporting two-sector ATPase

This is an abbreviated version!
For detailed information about H+-transporting two-sector ATPase, go to the full flat file.

Word Map on EC 7.1.2.2

Reaction

ATP
+
H2O
 + 4 H+[side 1] =
ADP
 +
phosphate
 + 4 H+[side 2]

Synonyms

15 kDa mediatophore protein, 32 kDa accessory protein, 59 kDa membrane-associated GTP-binding protein, A-ATP synthase, A-ATPase, A1A0-ATP synthase, A1AO ATP synthase, A6L, ApNa+-ATPase, ATP synthase, ATP synthase F1, ATP synthase proteolipid P1, ATP synthase proteolipid P2, ATP synthase proteolipid P3, ATP5alpha1, ATPase, ATPaseTb2, atpB, AtpZ, bacterial Ca2+/Mg2+ ATPase, BN59, C7-1 protein, cf1, CF1-ATPase, CGI-11, chloroplast ATP synthase, chloroplast ATPase, chlorpoplast ATP synthase, complex V, coupling factor CF1, coupling factors (F0,F1 and CF1), Dicyclohexylcarbodiimide-binding protein, Ductin, DVA41, EC 3.6.1.34, EC 3.6.3.14, ECF1, Ecto-F1Fo ATP synthase/F1 ATPase, ectopic FoF1 ATP synthase, F-type proton-translocating ATPase, F0F1 ATP synthase, F0F1-ATP synthase, F0F1-ATP synthase alpha, F0F1-ATPase, F0F1ATP synthase, F1, F1 ATPase, F1-ATP synthase, F1-ATPase, F1-ATPase beta subunit, F1-F0 ATP synthase, F1F0 ATP synthase, F1F0 ATPase, F1F0-ATP synthase, F1F0-ATPase, F1F0H+-ATPase, F1Fo, F1Fo ATP synthase, F1FO ATPase, F1FO-ATP synthase, F1FO-ATPase, FoF1, FoF1 ATP synthase, FoF1 ATPase, FoF1-ATP synthase, FoF1-ATPase, FoF1-ATPase/synthase, FoF1H+-ATPase/synthase, Fo·F1-H+-ATPase (synthase), H(+)-transporting ATP synthase, H+ FoF1-ATP synthase, H+-ATP synthase, H+-ATPase, H+-coupled ATP synthase, H+-translocating ATPase, H+-transporting ATP synthase, H+-transporting ATPase, H+-V-ATPase, HATPL, HO57, Invasion protein invC, Isoform HO68, Isoform VA68, Lipid-binding protein, M40, matpase, membrane-associated ATPase, mitochondrial ATPase, mitochondrial F(1)-ATPase, mitochondrial F0F1-ATP synthase, mitochondrial F1F0 ATP hydrolase, mitochondrial F1Fo-ATP synthase, mitochondrial FOF1 ATP synthase, mitochondrial H+-ATP synthase, More, My032 protein, Na+-dependent F1F0-ATP synthase, Oligomycin sensitivity conferral protein, OSCP, P31, P39, photosynthetic F1-ATPase, Physophilin, PKIWI505, plasma membrane V-ATPase, plasma membrane vacuolar H+-ATPase, Protein bellwether, proton translocating chloroplast ATP synthase, proton-translocating ATPase, rotary FOF1-ATPase, rotary molecular motor, Spa47, Stv1p, Sul-ATPase, Sul-ATPase alpha, Sul-ATPase beta, SUL-ATPase epsilon, Sul-ATPase gamma, T3SS ATPase, Tbeta, TF1, TF1-ATPase, TFoF1, tonoplast H+-ATPase, type III secretion-associated ATPase, uncB, UV-inducible PU4 protein, V-ATPase, V-ATPase 28 kDa accessory protein, V-ATPase 40 kDa accessory protein, V-ATPase 41 KDa accessory protein, V-ATPase 9.2 kDa membrane accessory protein, V-ATPase S1 accessory protein, V-pump, V-type ATPase/synthase, V-type H+-ATPase, V1VO ATPase, vacuolar (H+)-ATPase, vacuolar ATPase, vacuolar H(+)-ATPase, vacuolar H+-ATPase, vacuolar H+-pumping ATPase, vacuolar H+-translocating ATPase, vacuolar H+-transporting adenosine triphosphatase, vacuolar proton-translocating ATPase, vacuolar-type H+-ATPase, vacuolar-type H+-translocating ATPase, vacuolar-type proton pumping ATPase, vacuolar-type, proton-translocating ATPase enzyme complex, VEG100, VEG31, Vegetative protein 100, Vegetative protein 31, VHA, VHA-A, VHA16K, VMA8, Vma8p, Vph1p, YOPS secretion ATPase

ECTree

     7 Translocases
         7.1 Catalysing the translocation of hydrons
             7.1.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.1.2.2 H+-transporting two-sector ATPase

Purification

Purification on EC 7.1.2.2 - H+-transporting two-sector ATPase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
30fold by cholate washed vesicles followed by Triton X-100 solubilization and PEG 6000 precipitation
51fold by chromatographies, purified ATPase does not contain the F0-portion
-
alpha and beta subunits of F1-ATPase
-
alpha-subunit, beta-subunit, gamma-subunit, delta-subunit with glutathione S-transferase fused at the amino terminus and the epsilon-subunit with glutathione S-transferase fused at the amino terminus
-
ammonium sulfate precipitation and Superdex 200 gel filtration
Thermosynechococcus vestitus
-
by Ni-chelate affinity chromatography and gel filtration
-
complexes F1-wt and F1 deltasigma, purified in high yield and purity by high affinity chromatography, anionic exchange chromatography and GPC
-
deltaepsilon complex
-
F0 membrane domain
-
His-tagged cysteine-less F1F0 ATP synthase, 80 fold by Ni-NTA affinity chromatography
-
large-scale purification
-
native enzyme by gel filtration
native enzyme from mitochondria
-
native enzyme from thylakoid membranes by ammonium sulfate fractionation, dye-ligand affinity and anion exchange chromatography
Thermosynechococcus vestitus
-
native enzyme from thylakoid membranes by ammonium sulfate fractionation, sucrose density centrifugation, and anion exchange chromatography
native enzymes from plasma membranes and mitochondria, solubilization by Triton X-100
-
native H+ FoF1-ATP synthase complex from bovine heart mitochondria by detergent solubilization, ultracentrifugation, and ultrafiltration
-
Ni-NTA column chromatography, DEAE Toyopearl column chromatography, and phenyl-Toyopearl column chromatography
P0ABB0; P0ABB4; P0ABA6; P0A6E6
Ni-NTA column chromatography, Hitrap Q column chromatography, and Superdex 75 gel filtration
nickel affinity column chromatography and gel filtration
-
Q-Sepharose column chromatography and Superdex 200 gel filtration
-
recombinant F1Fo-ATP synthase in membrane vesicles from enzyme-deficient Escherichia coli mutant strain DK8
-
recombinant His-tagged chimeric alpha3beta3gammaredox complex by nickel affinity chromatography and gel filtration
recombinant His-tagged F1-ATPase from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography and gel filtration
-
recombinant His-tagged wild-type and mutant enzyme alpha, beta, and gamma subunits from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His10-tagged FoF1, from Echerichia coli strain DK8 by nickel affinity chromatography
-
recombinant monomeric Tbeta from F1Fo enzyme-deficient Escherichia coli strain DK 8 to homogeneity
-
recombinant N-terminally His10-tagged wild-type and mutant F1Fo from Escherichia coli and the in vitro protease-free protein synthesis, PURE, system by nickel affinity chromatography
-
recombinant soluble His3-tagged V1VO ATPase subunit A from Escherichia coli by immobilized metal affinity and ion exchange chromatography to homogeneity
-
recombinant wild-type and mutant enzymes
-
recombinant wild-type and mutant V-ATPase B subunits as maltose-binding protein fusion proteins from Escherichia coli
-
recombinant wild-type enzyme and subunit epsilon mutant EFoF1 from Escherichia coli strain RA1
-
single cysteine mutations of subunit b
-
Talon resin column chromatography and Sephacryl S100 gel filtration
-