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Literature summary for 7.1.2.2 extracted from

  • Nelson, N.
    Structure and function of chloroplast ATPase (1976), Biochim. Biophys. Acta, 456, 314-318.
    View publication on PubMed
Search for more literature for 7.1.2.2

Activating Compound

Activating Compound Comment Organism Structure
additional information activity is latent and must be unmasked by mild proteolysis with trypsin Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Euglena gracilis
ADP
-
 Spinacia oleracea
Hg2+
-
 Spinacia oleracea
Mg2+ 0.3 mM, 50% inhibition of Ca2+-dependent activity Spinacia oleracea
MnO4-
-
 Spinacia oleracea
SO42-
-
 Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Euglena gracilis 9507
-
chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Euglena gracilis
Ca2+ trypsin-induced ATPase activity of solubilized CF1 is dependent on the presence of Ca2+ Spinacia oleracea
Mg2+ ATPase activity of membrane bound CF1 is Mg2+-dependent Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
 x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
17500
-
 x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
37000
-
 x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
56000
-
 x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
59000
-
 x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea
325000
-
 equilibrium ultracentrifugation Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Euglena gracilis ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 Spinacia oleracea ADP + phosphate + H+/out
-
 ?
GTP + H2O + H+/in
-
 Euglena gracilis GDP + phosphate + H+/out
-
 ?
GTP + H2O + H+/in
-
 Spinacia oleracea GDP + phosphate + H+/out
-
 ?
ITP + H2O + H+/in
-
 Euglena gracilis IDP + phosphate + H+/out
-
 ?
ITP + H2O + H+/in
-
 Spinacia oleracea IDP + phosphate + H+/out
-
 ?

Subunits

Subunits Comment Organism
? x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE Spinacia oleracea