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Literature summary for 7.1.2.2 extracted from

  • Hossain, M.D.; Furuike, S.; Onoue, Y.; Adachi, K.; Yoshida, M.; Kinosita, K.
    Stimulation of F1-ATPase activity by sodium dodecyl sulfate (2010), Biochim. Biophys. Acta, 1797, 435-442.
    View publication on PubMed
Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged F1-ATPase in Escherichia coli strain Bl21(DE3) Bacillus sp. PS3

Inhibitors

Inhibitors Comment Organism Structure
MgADP- the enzyme is inhibited by tightly binding MgADP- and the inhibited fraction accumulates gradually until a steady state is reached that represents a dynamic equilibrium between active and MgADP-inhibited forms Bacillus sp. PS3
Sodium dodecyl sulfate
-
 Bacillus sp. PS3

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bacillus sp. PS3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+/in Bacillus sp. PS3
-
 ADP + phosphate + H+/out
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus sp. PS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged F1-ATPase from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography and gel filtration Bacillus sp. PS3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in
-
 Bacillus sp. PS3 ADP + phosphate + H+/out
-
 r
ATP + H2O + H+/in ATP binding is rate limiting at a concentration below 0.002 mM Bacillus sp. PS3 ADP + phosphate + H+/out
-
 r

Synonyms

Synonyms Comment Organism
F1-ATPase
-
 Bacillus sp. PS3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Bacillus sp. PS3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus sp. PS3