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Literature summary for 7.1.2.2 extracted from

  • Ferguson Scott, F.S.; Keis Stefani, K.S.; Cook Gregory, C.G.
    Biochemical and molecular characterization of a Na+-translocating F1Fo-ATPase from the thermoalkaliphilic bacterium Clostridium paradoxum (2006), J. Bacteriol., 188, 5045-5054.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.1.2.2

Application

Application Comment Organism
molecular biology F1FO-ATP synthase is a Na+-translocating ATPase used to generate an electrochemical gradient of Na+ that can drive other membrane-bound bioenergetic processes [Clostridium] paradoxum

Inhibitors

Inhibitors Comment Organism Structure
N,N'-dicyclohexylcarbodiimide 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100%; 0.2 mM can inhibit over 70% of activity after 20 min, which is pH dependent, 50 mM NaCl provides 40% protection against inhibition at pH 7.0, at pH 9.0 1 mM NaCl protects 100% [Clostridium] paradoxum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 ATP purified enzyme stimulated with Na+ at pH 7.0 and 9.0 [Clostridium] paradoxum
0.55
-
 ATP purified enzyme, Mg2+/ATP ratio maintained at 2:1 [Clostridium] paradoxum
1
-
 ATP
-
 [Clostridium] paradoxum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 [Clostridium] paradoxum 16020
-
vesicle
-
 [Clostridium] paradoxum 31982
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can function in place of MgCl2, although the affinity is much lower [Clostridium] paradoxum
Mg2+ dependent on [Clostridium] paradoxum
Na+ stimulates purified enzyme 3-4fold at pH 7.0 or 5-6fold at pH 9.0 with increasing concentrations of Na+ [Clostridium] paradoxum

Organism

Organism UniProt Comment Textmining
[Clostridium] paradoxum Q0ZS18 strain DSM7308
-
[Clostridium] paradoxum Q0ZS19 strain DSM7308
-
[Clostridium] paradoxum Q0ZS20 strain DSM7308
-
[Clostridium] paradoxum Q0ZS21 strain DSM7308
-
[Clostridium] paradoxum Q0ZS22 strain DSM7308
-
[Clostridium] paradoxum Q0ZS23 strain DSM7308
-
[Clostridium] paradoxum Q0ZS24 strain DSM7308
-
[Clostridium] paradoxum Q0ZS25 strain DSM7308
-
[Clostridium] paradoxum Q0ZS26 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS18 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS19 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS20 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS21 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS22 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS23 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS24 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS25 strain DSM7308
-
[Clostridium] paradoxum DSM 7308 Q0ZS26 strain DSM7308
-

Purification (Commentary)

Purification (Comment) Organism
30fold by cholate washed vesicles followed by Triton X-100 solubilization and PEG 6000 precipitation [Clostridium] paradoxum

Storage Stability

Storage Stability Organism
-80°C, Tris-Cl, pH 8.0, 2 months [Clostridium] paradoxum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + H+ low rates of ATP synthesis [Clostridium] paradoxum ATP + H2O
-
 ?
ADP + phosphate + H+ low rates of ATP synthesis [Clostridium] paradoxum DSM 7308 ATP + H2O
-
 ?
ATP + H2O + H+/in
-
 [Clostridium] paradoxum ADP + phosphate + H+/out
-
 ?
ATP + H2O + H+/in
-
 [Clostridium] paradoxum DSM 7308 ADP + phosphate + H+/out
-
 ?

Subunits

Subunits Comment Organism
More 9 subunits as calculated from DNA sequence [Clostridium] paradoxum

Synonyms

Synonyms Comment Organism
F1FO-ATP synthase
-
 [Clostridium] paradoxum
F1FO-ATPase
-
 [Clostridium] paradoxum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 [Clostridium] paradoxum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 stable for 4 days, retaining near 100% of the starting activity, in contrast, the purified enzyme rapidly loses activity overnight and retains only 25% of its starting activity after 5 days [Clostridium] paradoxum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6 8.5
-
 [Clostridium] paradoxum

pH Range

pH Minimum pH Maximum Comment Organism
7 9 enzyme retains ca. 20% of its maximum activity at below pH 7.0 [Clostridium] paradoxum