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Literature summary for 7.1.2.2 extracted from

  • Pulido, N.O.; Salcedo, G.; Perez-Hernandez, G.; Jose-Nunez, C.; Velazquez-Campoy, A.; Garcia-Hernandez, E.
    Energetic effects of magnesium in the recognition of adenosine nucleotides by the F1-ATPase beta subunit (2010), Biochemistry, 49, 5258-5268.
    View publication on PubMed
Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of monomeric Tbeta in F1Fo enzyme-deficient Escherichia coli strain DK 8 Bacillus sp. PS3

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bacillus sp. PS3 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, analysis of binding of F1 monomer to Mg2+-free and Mg2+-bound adenosine nucleotides using high-precision isothermal titration calorimetry, structural-energetic analysis suggests that Tbeta adopts a more closed conformation when it is bound to Mg-ATP than to ATP or Mg-ADP, in agreement with recently published NMR data, analysis of Mg2+ energetic effects for the free energy change of F1 catalytic sites, in the framework of bi- or tri-site binding models, overview Bacillus sp. PS3

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP + phosphate + H+/out Bacillus sp. PS3
-
 ATP + H2O + H+/in
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus sp. PS3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant monomeric Tbeta from F1Fo enzyme-deficient Escherichia coli strain DK 8 to homogeneity Bacillus sp. PS3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphate + H+/out
-
 Bacillus sp. PS3 ATP + H2O + H+/in
-
 r
ADP + phosphate + H+/out nucleotide-induced conformational changes in beta subunits are considered to be the essential driving force for rotational catalysis in F1 Bacillus sp. PS3 ATP + H2O + H+/in
-
 r

Synonyms

Synonyms Comment Organism
F1-ATPase beta subunit
-
 Bacillus sp. PS3
Tbeta
-
 Bacillus sp. PS3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bacillus sp. PS3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Bacillus sp. PS3