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7.1.2.2: H+-transporting two-sector ATPase

This is an abbreviated version!
For detailed information about H+-transporting two-sector ATPase, go to the full flat file.

Word Map on EC 7.1.2.2

Reaction

ATP
+
H2O
 + 4 H+[side 1] =
ADP
 +
phosphate
 + 4 H+[side 2]

Synonyms

15 kDa mediatophore protein, 32 kDa accessory protein, 59 kDa membrane-associated GTP-binding protein, A-ATP synthase, A-ATPase, A1A0-ATP synthase, A1AO ATP synthase, A6L, ApNa+-ATPase, ATP synthase, ATP synthase F1, ATP synthase proteolipid P1, ATP synthase proteolipid P2, ATP synthase proteolipid P3, ATP5alpha1, ATPase, ATPaseTb2, atpB, AtpZ, bacterial Ca2+/Mg2+ ATPase, BN59, C7-1 protein, cf1, CF1-ATPase, CGI-11, chloroplast ATP synthase, chloroplast ATPase, chlorpoplast ATP synthase, complex V, coupling factor CF1, coupling factors (F0,F1 and CF1), Dicyclohexylcarbodiimide-binding protein, Ductin, DVA41, EC 3.6.1.34, EC 3.6.3.14, ECF1, Ecto-F1Fo ATP synthase/F1 ATPase, ectopic FoF1 ATP synthase, F-type proton-translocating ATPase, F0F1 ATP synthase, F0F1-ATP synthase, F0F1-ATP synthase alpha, F0F1-ATPase, F0F1ATP synthase, F1, F1 ATPase, F1-ATP synthase, F1-ATPase, F1-ATPase beta subunit, F1-F0 ATP synthase, F1F0 ATP synthase, F1F0 ATPase, F1F0-ATP synthase, F1F0-ATPase, F1F0H+-ATPase, F1Fo, F1Fo ATP synthase, F1FO ATPase, F1FO-ATP synthase, F1FO-ATPase, FoF1, FoF1 ATP synthase, FoF1 ATPase, FoF1-ATP synthase, FoF1-ATPase, FoF1-ATPase/synthase, FoF1H+-ATPase/synthase, Fo·F1-H+-ATPase (synthase), H(+)-transporting ATP synthase, H+ FoF1-ATP synthase, H+-ATP synthase, H+-ATPase, H+-coupled ATP synthase, H+-translocating ATPase, H+-transporting ATP synthase, H+-transporting ATPase, H+-V-ATPase, HATPL, HO57, Invasion protein invC, Isoform HO68, Isoform VA68, Lipid-binding protein, M40, matpase, membrane-associated ATPase, mitochondrial ATPase, mitochondrial F(1)-ATPase, mitochondrial F0F1-ATP synthase, mitochondrial F1F0 ATP hydrolase, mitochondrial F1Fo-ATP synthase, mitochondrial FOF1 ATP synthase, mitochondrial H+-ATP synthase, More, My032 protein, Na+-dependent F1F0-ATP synthase, Oligomycin sensitivity conferral protein, OSCP, P31, P39, photosynthetic F1-ATPase, Physophilin, PKIWI505, plasma membrane V-ATPase, plasma membrane vacuolar H+-ATPase, Protein bellwether, proton translocating chloroplast ATP synthase, proton-translocating ATPase, rotary FOF1-ATPase, rotary molecular motor, Spa47, Stv1p, Sul-ATPase, Sul-ATPase alpha, Sul-ATPase beta, SUL-ATPase epsilon, Sul-ATPase gamma, T3SS ATPase, Tbeta, TF1, TF1-ATPase, TFoF1, tonoplast H+-ATPase, type III secretion-associated ATPase, uncB, UV-inducible PU4 protein, V-ATPase, V-ATPase 28 kDa accessory protein, V-ATPase 40 kDa accessory protein, V-ATPase 41 KDa accessory protein, V-ATPase 9.2 kDa membrane accessory protein, V-ATPase S1 accessory protein, V-pump, V-type ATPase/synthase, V-type H+-ATPase, V1VO ATPase, vacuolar (H+)-ATPase, vacuolar ATPase, vacuolar H(+)-ATPase, vacuolar H+-ATPase, vacuolar H+-pumping ATPase, vacuolar H+-translocating ATPase, vacuolar H+-transporting adenosine triphosphatase, vacuolar proton-translocating ATPase, vacuolar-type H+-ATPase, vacuolar-type H+-translocating ATPase, vacuolar-type proton pumping ATPase, vacuolar-type, proton-translocating ATPase enzyme complex, VEG100, VEG31, Vegetative protein 100, Vegetative protein 31, VHA, VHA-A, VHA16K, VMA8, Vma8p, Vph1p, YOPS secretion ATPase

ECTree

     7 Translocases
         7.1 Catalysing the translocation of hydrons
             7.1.2 Linked to the hydrolysis of a nucleoside triphosphate
                7.1.2.2 H+-transporting two-sector ATPase

Cloned

Cloned on EC 7.1.2.2 - H+-transporting two-sector ATPase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
A1AO ATP synthase is functionally produced in the F1FO ATP synthase-negative strain Escherichia coli DK8
-
alpha-subunit, expression in Escherichia coli
-
ATP2 gene, coding for the beta subunit of the mitochondrial F1-ATPase
ATPase genetically modified to include a His6 Ni affinity tag on the amino end of the mature beta-subunit, imported into mitochondrion, expression of the the purified chimeric enzyme in Escherichia coli DMY301
-
beta and epsilon subunits
-
construction of an expression plasmid and overexpression of the alpha-subunit, beta-subunit, gamma-subunit, delta-subunit with glutathione S-transferase fused at the amino terminus and the epsilon-subunit with glutathione S-transferase fused at the amino terminus
-
expressed in Escherichia coli BL21 strain T7 Express lysY
-
expressed in Escherichia coli BL21(DE3) cells
Thermosynechococcus vestitus
-
expressed in Escherichia coli mutant DK8 deficient in ATP synthase and in Synechococcus sp. strain PCC 7942
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli XL1-Blue MRF' cells
expressed in seedlings of Nicotiana tabacum cultivar NC89
-
expression in enzyme-deficient Escherichia coli mutant strain DK8
-
expression of a hybrid enzyme, formed by a mouse E1 isozyme and Saccharomyces cerevisiae subunits, in DELTAvma4 cells
-
expression of a hybrid enzyme, formed by a mouse E1 isozyme and yeast subunits, in DELTAvma4 cells
-
expression of at subunit beta N-terminally His10-tagged wild-type and mutant F1Fo in Escherichia coli and by the in vitro protease-free protein synthesis, PURE, system
-
expression of Atp6p in HeLa cells depleted of the F1 beta subunit. Instead of being translationally downregulated, HeLa cells lacking F1 degrade Atp6p, thereby preventing proton leakage across the inner membrane
expression of C-terminally c-Myc-tagged wild-type F1b eta or F1betaK212Q mutants in HEK-293 cells
-
expression of FoF1, tagged with His10 at the beta-subunit N-terminus, in Escherichia coli strain DK8
-
expression of His-tagged enzyme mutants R364K, C193S, and C193S/R364K and of His-tagged hybrid mutants in Escherichia coli
-
expression of His-tagged F1-ATPase in Escherichia coli strain Bl21(DE3)
-
expression of His-tagged holoenzyme in Escherichia coli strain DK8
-
expression of His-tagged wild-type and mutant enzyme alpha, beta, and gamma subunits in Escherichia coli strain M15
expression of monomeric Tbeta in F1Fo enzyme-deficient Escherichia coli strain DK 8
-
expression of recombinant His-tagged chimeric alpha3beta3gammaredox complex
expression of recombinant TF0F1 with a histidine tag of 10 residues at the N terminus of the beta subunit in Escherichia coli
-
expression of subunit F1beta in Escherichia coli strain JM103, and of the beta subunit and fragments in Escherichia coli strain BL21(DE3)
-
expression of the soluble His3-tagged V1VO ATPase subunit A of in Escherichia coli
-
expression of the the cyano-epsilon, CF1-epsilon and the chimeric epsilon subunits in Escherichia coli strain BL21(DE3) as soluble proteins
Thermosynechococcus vestitus
-
expression of wild-type and mutant enzymes
-
expression of wild-type and mutant enzymes complex in strain DK8
-
expression of wild-type and mutant enzymes in Escherichia coli strain DK8
-
expression of wild-type and mutant V-ATPase B subunits as maltose-binding protein fusion proteins in Escherichia coli
-
expression of wild-type FoF1 and subunit epsilon mutant EFoF1 in Escherichia coli strain RA1
-
F1 moiety from the atp operon into vector pTrc99A, overexpressed in Escherichia coli RNE41 in the two variant complexes F1-wt and F1 deltasigma
-
gene atpZ, encoded in the atp operon, sequence comparison, overview
Alkalihalophilus pseudofirmus
gene VHA-A encoding the catalytic subunit A, expression analysis
-
genomic library construction, cloning of the gene encoding the alpha-subunit of the enzyme, DNA and amino acid sequence determination and analysis, sequence comparisons to F1-ATPAses, overview
-
genomic library construction, cloning of the gene encoding the beta-subunit of the enzyme, DNA and amino acid sequence determination and analysis, sequence comparisons to the alpha-subunit sequence, and other F1-ATPase alpha-and beta-subunits, overview
-
His-tagged enzyme is expressed in FoF1-deficient Escherichia coli B834(DE)cells
P0ABB0; P0ABB4; P0ABA6; P0A6E6
mutant enzymes are expressed in Kluyveromyces lactis mutant lacking the gamma rotor
-
mutant plasmids expressed in Escherichia coli strain JM103
-
overexpressed in Escherichia coli
overexpression of mutant enzymes in Escherichia coli and reconstitution of recombinant epsilon proteins with CF1(-epsilon) and epsilon-deficient thylakoid membranes
-
phylogenetic analysis
sequence comparison, overview
strain ATCC3020 in Escherichia coli-derived in vitro transcription-translation system
-
subunit E, DNA and amino acid sequence determination and anaylsis, phylogenetic analysis, expession analysis under salt stress, overview
-
the catalytic subunit A of the archaeal-type H+-ATPase is cloned and expressed in Escherichia coli
transient expression of GFP-tagged ATP5B protein on cell surface and mitochondria in carcinoma cell lines, i.e. Hep-G2 cells, A-549 cells, 95-D cells, L-02 cells, and HEK-293 cells, the ectopic expression of ATP synthase is a consequence of translocation from the mitochondria
-
transient overexpression of FOF1-ATP synthase alpha in human neuroblastoma SH-SY5Y cells
-