Cloned (Comment) | Organism |
---|---|
expression in enzyme-deficient Escherichia coli mutant strain DK8 | Bacillus sp. PS3 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacillus sp. PS3 | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bacillus sp. PS3 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | Bacillus sp. PS3 | - |
ADP + phosphate + H+/out | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. PS3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant F1Fo-ATP synthase in membrane vesicles from enzyme-deficient Escherichia coli mutant strain DK8 | Bacillus sp. PS3 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + H2O + 4 H+[side 1] = ADP + phosphate + 4 H+[side 2] | ATP synthase uses a unique rotary mechanism to couple ATP synthesis and hydrolysis to transmembrane proton translocation. As part of the synthesis mechanism, the torque of the rotor has to be converted into conformational rearrangements of the catalytic binding sites on the stator to allow synthesis and release of ATP. The gamma subunit of the rotor plays a central role in the energy conversion. The N-terminal helix alone is able to fulfill the function of full-length gamma in ATP synthesis as long as it connects to the rest of the rotor. This connection can occur via the epsilon subunit. No direct contact between epsilon and the gamma ring seems to be required. The epsilon subunit of the rotor exists in two different conformations during ATP synthesis and ATP hydrolysis. Reaction mechanism, overview | Bacillus sp. PS3 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in | - |
Bacillus sp. PS3 | ADP + phosphate + H+/out | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ATP synthase | - |
Bacillus sp. PS3 |
F1Fo | - |
Bacillus sp. PS3 |
F1FO-ATP synthase | - |
Bacillus sp. PS3 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
assay at | Bacillus sp. PS3 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus sp. PS3 |