Literature summary for 7.1.2.2 extracted from

Sunamura, E.; Konno, H.; Imashimizu, M.; Mochimaru, M.; Hisabori, T.
A conformational change of the gamma subunit indirectly regulates the activity of cyanobacterial F1-ATPase (2012), J. Biol. Chem., 287, 38695-38704.

Search for more literature for 7.1.2.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thermosynechococcus vestitus

Protein Variants

Protein Variants	Comment	Organism
A28C/L267C	the mutant shows strongly reduced activity compared to the wild type enzyme	Thermosynechococcus vestitus
A35C/A264C	the mutant shows increased ATP hydrolysis activity compared to the wild type enzyme	Thermosynechococcus vestitus
V32C/A268C	the mutant shows by about 4fold increased ATP hydrolysis activity compared to the wild type enzyme	Thermosynechococcus vestitus
V39C/A264C	the mutant shows by about 2.5fold increased ATP hydrolysis activity compared to the wild type enzyme	Thermosynechococcus vestitus
V39C/A264C	the mutant shows greatly increased ATP hydrolysis activity compared to the wild type enzyme	Thermosynechococcus vestitus

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Thermosynechococcus vestitus
additional information	the epsilon subunit of the enzyme inhibits ATP hydrolysis activity, while the global conformational change of the gamma subunit indirectly regulates complex activity by changing both ADP inhibition and epsilon inhibition	Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + H+/in	Thermosynechococcus vestitus	-	ADP + phosphate + H+/out	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermosynechococcus vestitus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation and Superdex 200 gel filtration	Thermosynechococcus vestitus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + H+/in	-	Thermosynechococcus vestitus	ADP + phosphate + H+/out	-	?

Synonyms

Synonyms	Comment	Organism
F1-ATPase	-	Thermosynechococcus vestitus

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Release 2023.1 (January 2023)