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Literature summary for 7.1.2.2 extracted from

  • Shi, X.B.; Wei, J.M.; Shen, Y.K.
    Effects of sequential deletions of residues from the N- or C-terminus on the functions of epsilon subunit of the chloroplast ATP synthase (2001), Biochemistry, 40, 10825-10831.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of mutant enzymes in Escherichia coli and reconstitution of recombinant epsilon proteins with CF1(-epsilon) and epsilon-deficient thylakoid membranes Spinacia oleracea

Protein Variants

Protein Variants Comment Organism
epsilonC6S mutant inhibits ATPase activita as potently as the epsilon-wild-type Spinacia oleracea
epsilonDELTAC10 mutant loses more than 80% of the inhibitory activity towards soluble ATPase Spinacia oleracea
epsilonDELTAC6 mutant loses 40% of the inhibitory activity towards soluble ATPase Spinacia oleracea
epsilonDELTAC7 mutant loses about 70% of the inhibitory activity towards soluble ATPase Spinacia oleracea
epsilonDELTAC8 mutant loses about 70% of the inhibitory activity towards soluble ATPase Spinacia oleracea
epsilonDELTAC9 mutant loses about 70% of the inhibitory activity towards soluble ATPase Spinacia oleracea
epsilonDELTAN1 deletion has only marginal effect on the maximum ATPase-inhibitory activity Spinacia oleracea
epsilonDELTAN2 deletion has only marginal effect on the maximum ATPase-inhibitory activity Spinacia oleracea
epsilonDELTAN3 mutation decreases its inhibitory activity towards ATPase activity significantly Spinacia oleracea
epsilonDELTAN4 mutant loses most of the inhibitory activity towards ATPase in solution, 45% loss of inhibitory activity on membrane-bound ATPase, interaction with gamma subunits is lowered Spinacia oleracea
epsilonDELTAN5 mutant loses most of the inhibitory activity towards ATPase in solution, 50% loss of inhibitorty activity on membrane-bound ATPase, interaction with gamma subunits is lowered Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information in chlorpoplast ATP synthase, both the N-terminus and C-terminus of the epsilon subunit show importance in regulation of the ATPase activity. The N-terminus of the epsilon subunit is more important for its interaction with gamma and some CF0 subunits, and crucial for the blocking of the proton leakage Spinacia oleracea ?
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Subunits

Subunits Comment Organism
More in chlorpoplast ATP synthase, both the N-terminus and C-terminus of the epsilon subunit show importance in regulation of the ATPase activity. The N-terminus of the epsilon subunit is more important for its interaction with gamma and some CF0 subunits, and crucial for the blocking of the proton leakage Spinacia oleracea

Synonyms

Synonyms Comment Organism
chloroplast ATPase
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Spinacia oleracea
chlorpoplast ATP synthase
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Spinacia oleracea