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Literature summary for 7.1.2.2 extracted from
- Mitochondrial and chloroplast ATPases (1974), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 10, 375-429.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-dinitrophenol | stimulates | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Bos taurus | |
| ADP | - |
Euglena gracilis | |
| ADP | - |
Rattus norvegicus | |
| ADP | - |
Spinacia oleracea | |
| Aurovertin | - |
Bos taurus | |
| Aurovertin | - |
Saccharomyces cerevisiae | |
| I2 | - |
Bos taurus | |
| Mg2+ | 0.3 mM, 50% inhibition of Ca2+-dependent activity | Spinacia oleracea | |
| N,N'-dicyclohexylcarbodiimide | - |
Bos taurus | |
| N,N'-dicyclohexylcarbodiimide | - |
Saccharomyces cerevisiae | |
| Tetranitromethane | - |
Bos taurus | |
| Trypsin | - |
Euglena gracilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.097 | - |
ATP | - |
Euglena gracilis | |
| 0.11 | - |
ATP | 2-(N-morpholino)ethane sulfonic acid buffer | Spinacia oleracea | |
| 0.25 | - |
ATP | - |
Saccharomyces cerevisiae | |
| 0.315 | - |
ATP | pH 8, membrane-bound enzyme | Bos taurus | |
| 0.79 | - |
ATP | pH 8, soluble enzyme | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Euglena gracilis | 9507 | - |
| chloroplast | - |
Spinacia oleracea | 9507 | - |
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
| mitochondrion | - |
Bos taurus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required | Euglena gracilis | |
| Ca2+ | required | Spinacia oleracea | |
| Ca2+ | can replace Mg2+ or Co2+ | Bos taurus | |
| Co2+ | absolute requirement for a divalent metal ion, Mg2+ or Co2+ | Bos taurus | |
| Fe2+ | can replace Mg2+ or Co2+ | Bos taurus | |
| Mg2+ | - |
Spinacia oleracea | |
| Mg2+ | absolute requirement for a divalent metal ion, Mg2+ or Co2+ | Bos taurus | |
| Mn2+ | can replace Mg2+ or Co2+ | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 5700 | - |
subunits of F1, x * 54000 + x * 50000 + x * 33000 + x * 17000 + x * 5700, SDS-PAGE | Bos taurus |
| 13000 | - |
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE | Spinacia oleracea |
| 17000 | - |
subunits of F1, x * 54000 + x * 50000 + x * 33000 + x * 17000 + x * 5700, SDS-PAGE | Bos taurus |
| 17500 | - |
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE | Spinacia oleracea |
| 33000 | - |
subunits of F1, x * 54000 + x * 50000 + x * 33000 + x * 17000 + x * 5700, SDS-PAGE | Bos taurus |
| 37000 | - |
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE | Spinacia oleracea |
| 50000 | - |
subunits of F1, x * 54000 + x * 50000 + x * 33000 + x * 17000 + x * 5700, SDS-PAGE | Bos taurus |
| 54000 | - |
subunits of F1, x * 54000 + x * 50000 + x * 33000 + x * 17000 + x * 5700, SDS-PAGE | Bos taurus |
| 56000 | - |
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE | Spinacia oleracea |
| 59000 | - |
x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE | Spinacia oleracea |
| 325000 | - |
equilibrium ultracentrifugation | Spinacia oleracea |
| 360000 | 384000 | equilibrium sedimentation | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Euglena gracilis | - |
- |
- |
| Magnusiomyces magnusii | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | tests for glucosamine, galactosamine and fatty acids in F1 are negative | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
- |
Saccharomyces cerevisiae |
- |
Bos taurus |
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
| heart | - |
Bos taurus | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 100 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 22°C, 0.25 M sucrose, 2 mM EDTA, 4 mM ATP, 50 mM Tris-sulfate, pH 8, stable for several days | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + H+/in | - |
Rattus norvegicus | ADP + phosphate + H+/out | - |
? | |
| ATP + H2O + H+/in | - |
Saccharomyces cerevisiae | ADP + phosphate + H+/out | - |
? | |
| ATP + H2O + H+/in | - |
Bos taurus | ADP + phosphate + H+/out | - |
? | |
| ATP + H2O + H+/in | - |
Euglena gracilis | ADP + phosphate + H+/out | - |
? | |
| ATP + H2O + H+/in | - |
Spinacia oleracea | ADP + phosphate + H+/out | - |
? | |
| ATP + H2O + H+/in | - |
Magnusiomyces magnusii | ADP + phosphate + H+/out | - |
? | |
| dATP + H2O + H+/in | - |
Euglena gracilis | dADP + phosphate + H+/out | - |
? | |
| GTP + H2O + H+/in | - |
Rattus norvegicus | GDP + phosphate + H+/out | - |
? | |
| GTP + H2O + H+/in | - |
Bos taurus | GDP + phosphate + H+/out | - |
? | |
| GTP + H2O + H+/in | - |
Euglena gracilis | GDP + phosphate + H+/out | - |
? | |
| GTP + H2O + H+/in | 17% of the activity with ATP | Spinacia oleracea | GDP + phosphate + H+/out | - |
? | |
| ITP + H2O + H+/in | - |
Rattus norvegicus | IDP + phosphate + H+/out | - |
? | |
| ITP + H2O + H+/in | - |
Bos taurus | IDP + phosphate + H+/out | - |
? | |
| ITP + H2O + H+/in | - |
Euglena gracilis | IDP + phosphate + H+/out | - |
? | |
| ITP + H2O + H+/in | 17% of the activity with ATP | Spinacia oleracea | IDP + phosphate + H+/out | - |
? | |
| UTP + H2O + H+/in | - |
Bos taurus | UDP + phosphate + H+/out | - |
? | |
| UTP + H2O + H+/in | 3% of the activity with ATP | Spinacia oleracea | UDP + phosphate + H+/out | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | subunits of F1, x * 54000 + x * 50000 + x * 33000 + x * 17000 + x * 5700, SDS-PAGE | Bos taurus |
| ? | x * 59000 + x * 56000 + x * 37000 + x * 17500 + x * 13000, SDS-PAGE | Spinacia oleracea |
| More | - |
Rattus norvegicus |
| More | - |
Saccharomyces cerevisiae |
| More | - |
Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
dissociation of the enzyme molecule at low temperatures, protection by chloroplast lipids | Spinacia oleracea |
| additional information | - |
enzyme is stable in the cold | Euglena gracilis |
- |
10 | - |
Euglena gracilis |
- |
10 | - |
Spinacia oleracea |
- |
10 | dissociation and loss of activity at low temperatures is accelerated in increasing order by I-, NO3-, Br-, Cl-, SO42- | Bos taurus |
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