4.6.1.24: ribonuclease T1
This is an abbreviated version!
For detailed information about ribonuclease T1, go to the full flat file.
Word Map on EC 4.6.1.24
-
4.6.1.24
-
pancreatic
-
lysozyme
-
refolding
-
amide
-
trna
-
nucleic
-
alpha-helix
-
angiogenin
-
single-stranded
-
dnase
-
ribonucleolytic
-
isomerization
-
guanosine
-
cytidine
-
ribonucleoproteins
-
myoglobin
-
subsite
-
two-state
-
globular
-
amyloliquefaciens
-
ribonucleic
-
enthalpy
-
seminal
-
native-like
-
s-protein
-
phosphodiester
-
polynucleotide
-
intermedius
-
micrococcal
-
transesterification
-
polycation
-
nucleases
-
monolith
-
oligoribonucleotides
-
trnaphe
-
anticodon
-
alpha-lactalbumin
-
dinucleoside
-
domain-swapped
-
beta-lactoglobulin
-
eosinophil-derived
-
rna-protein
-
3'-phosphate
-
3'-terminal
-
polyu
-
pumilus
-
dimethacrylate
-
biotechnology
-
drug development
-
polyribonucleotides
-
medicine
-
transphosphorylation
-
burial
- 4.6.1.24
- pancreatic
- lysozyme
-
refolding
- amide
- trna
- nucleic
-
alpha-helix
-
angiogenin
-
single-stranded
- dnase
-
ribonucleolytic
-
isomerization
- guanosine
- cytidine
- ribonucleoproteins
- myoglobin
-
subsite
-
two-state
-
globular
- amyloliquefaciens
-
ribonucleic
-
enthalpy
-
seminal
-
native-like
- s-protein
-
phosphodiester
- polynucleotide
- intermedius
-
micrococcal
-
transesterification
- polycation
- nucleases
-
monolith
- oligoribonucleotides
- trnaphe
-
anticodon
- alpha-lactalbumin
- dinucleoside
-
domain-swapped
- beta-lactoglobulin
-
eosinophil-derived
-
rna-protein
- 3'-phosphate
-
3'-terminal
- polyu
- pumilus
-
dimethacrylate
- biotechnology
- drug development
- polyribonucleotides
- medicine
-
transphosphorylation
-
burial
Reaction
Synonyms
Aspergillus oryzae ribonuclease, barnase, Binase, EC 2.7.7.26, EC 3.1.27.3, EC 3.1.4.8, Gln25-RNase T1, guanyl-preferring RNase, Guanyl-specific RNase, guanylate endoribonuclease, Guanyloribonuclease, More, MycRne, Nuclease, guanyloribo-, Nuclease, ribo-, Aspergillus oryzae, ribonuclease, ribonuclease A, Ribonuclease C2, Ribonuclease Ch, ribonuclease E/G, Ribonuclease F1, ribonuclease G, Ribonuclease guaninenucleotido-2'-transferase (cyclizing), Ribonuclease N1, Ribonuclease N3, ribonuclease NT, ribonuclease Pb2, Ribonuclease PP1, Ribonuclease SA, ribonuclease Sa2, ribonuclease T1, Ribonuclease U1, RNase A, RNase E/G, RNase F1, RNase Fl1, RNase Fl2, RNase G, RNase He1, RNase Ms, RNase N1, RNase N2, RNase NT, RNase Pb1, RNase Pb2, RNase Pc, RNase Po1, RNase Pol, RNase Sa, RNase Sa2, RNase Sa3, RNase St, RNase T1, RNase T1 RV, RNase T1-R2, RNase Th1, RNase U1, Slr1129
ECTree
4.6.1.24 ribonuclease T1Advanced search results
results (
results (Substrates Products
Substrates Products on EC 4.6.1.24 - ribonuclease T1
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
adenylyl-3',5'-cytidine
adenosine-2',3'-cyclic phosphate + cytidine
-
ApC
-
-
?
guanosine-2',3'-cyclic phosphate + H2O
guanosine 3'-phosphate
-
hydrolysis
-
-
?
guanylyl(3',5') adenosine + H2O
?
shift in nucleotide conformational equilibrium contributes to increased rate of catalysis of guanylyl(3',5') adenosine 3'-monophosphate versus guanylyl(3',5') adenosine
-
-
?
guanylyl(3',5') adenosine 3'-monophosphate + H2O
?
shift in nucleotide conformational equilibrium contributes to increased rate of catalysis of guanylyl(3',5') adenosine 3'-monophosphate versus guanylyl(3',5') adenosine
-
-
?
guanylyl-3',5'-cytidine
guanosine-2',3'-cyclic phosphate + cytidine
-
Transphosphorylation reaction
-
-
?
guanylyl-3',5'-cytidine + H2O
guanosine 3'-phosphate + cytidine
-
GpC
-
-
?
guanylyl-3',5'-uridine
guanosine-2',3'-cyclic phosphate + uridine
-
Transphosphorylation reaction with GpU or the diastereomers resulting from thio-substitution of a nonbridging oxygen of GpU, RpGp(S)U and SpGp(S)U as substrates. SpGp(S)U is a very poor substrate for wild type enzyme
-
-
?
[RNA] containing guanosine + H2O
an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment]
-
energetics of ribonuclease catalysis
-
-
?
adenylyl-3',5'-cytidine + H2O
adenosine 3'-phosphate + cytidine
-
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W
-
-
?
adenylyl-3',5'-cytidine + H2O
adenosine 3'-phosphate + cytidine
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV)
-
-
?
guanosine 2',3'-cyclic phosphate + H2O
guanosine-3'-phosphate
-
-
-
-
?
guanosine 2',3'-cyclic phosphate + H2O
guanosine-3'-phosphate
-
Ustilago sphaerogena, RNase U1
-
-
?
guanylyl-3',5'-cytidine + H2O
?
-
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W
-
-
?
guanylyl-3',5'-cytidine + H2O
?
kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV)
-
-
?
RNA + H2O
?
-
two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates
-
-
?
RNA + H2O
?
-
identification of RNase T1 cleavage fragments of Bacillus megaterium ribosomal 5S RNA
-
-
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
essential requirements: keto group at 6 position, trivalent nitrogen at 7 position of purine base
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
essential requirements: keto group at 6 position, trivalent nitrogen at 7 position of purine base
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
first step (r): cleavage of internucleotide bonds between 3'-guanylic acid groups and the 5'-hydroxyl groups of the adjacent nucleotides with the intermediary formation of guanosine 2',3'-cyclic phosphate, second step (ir): hydrolysis of cyclic phosphate to produce 3'-guanylate
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
first step (r): cleavage of internucleotide bonds between 3'-guanylic acid groups and the 5'-hydroxyl groups of the adjacent nucleotides with the intermediary formation of guanosine 2',3'-cyclic phosphate, second step (ir): hydrolysis of cyclic phosphate to produce 3'-guanylate
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
specificity
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
specificity
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
RNA + H2O
Hydrolyzed RNA
-
-
3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp
?
single-stranded RNA + H2O
?
the enzyme specifically hydrolyzes single-stranded RNA at G sites. RNase T1 does not recognize sites containing 8-oxo-7,8-dihydroguanosine
-
-
?
single-stranded RNA + H2O
?
the enzyme specifically hydrolyzes single-stranded RNA at G sites. RNase T1 does not recognize sites containing 8-oxo-7,8-dihydroguanosine
-
-
?
additional information
?
-
-
synthesis of guanylylnucleosides, oligoguanylate, guanosine-containing oligonucleotides with (3'-5')phosphodiester bonds, synthetic reaction can lead to the formation of unnatural (2'-5')-phosphodiester bonds, not: DNA, L-guanosine 2',3'-cyclic phosphate, L-inosine 2',3'-cyclic phosphate
-
-
?
additional information
?
-
-
heteroduplex of ribooligonucleotides are substrates for the enzyme
-
-
?
additional information
?
-
-
the backbone dynamics of RNase T1, complexed with the productive substrate exo-cGPS isomer, in comparison to the RNase T1, complexed with the nonproductive substrate 3'GMP, is analyzed
-
-
?
additional information
?
-
-
the enzyme cleaves single-stranded RNA by catalyzing the hydrolysis of phosphodiester bonds specifically at the 3'-side of guanosine nucleotides
-
-
?
additional information
?
-
-
the enzyme hydrolyzes specifically the 3'-phosphodiester bond of guanylic acid in RNA, mechanism of interaction of RNase T1 with its substrates, overview. His40 and His92 are deduced to be involved in the active site, overview. Carboxymethylated-RNase T1 possesses almost the same binding ability toward guanosine as intact RNase T1, whereas the binding ability toward 2' or 3'-guanylic acid is considerably lowered by carboxymethylation of Glu58
-
-
?
additional information
?
-
-
barnase catalyses the overall hydrolysis of single-stranded RNA preferentially at guanylyl residues, yielding new guanosine 3'-phosphate and 5'-OH ends, in a two-step process with cleavage of the RNA chain by transesterification of a 5'-phosphoester bond to form a guanosine 2',3'-cyclic phosphate terminus in the first step, followed by its hydrolysis to a 3'-phosphate product in the second independent step
-
-
?
additional information
?
-
in vivo binase affects the quantity of proapoptotic and antiapoptotic mRNAs, as expression of the p53 and hSK4 genes is increased and expression of the bcl-2 gene is reduced
-
-
?
additional information
?
-
-
in vivo binase affects the quantity of proapoptotic and antiapoptotic mRNAs, as expression of the p53 and hSK4 genes is increased and expression of the bcl-2 gene is reduced
-
-
?
additional information
?
-
-
binase catalyses the overall hydrolysis of single-stranded RNA preferentially at guanylyl residues, yielding new guanosine 3'-phosphate and 5'-OH ends, in a two-step process with cleavage of the RNA chain by transesterification of a 5'-phosphoester bond to form a guanosine 2',3'-cyclic phosphate terminus in the first step, followed by its hydrolysis to a 3'-phosphate product in the second independent step
-
-
?
additional information
?
-
-
endonucleolytic activity is assayed using derivates of BR13, 5'-GGGACAGUAUUUG-3', a model oligonucleotide substrate for studies of RNase E and RNase G
-
-
?
additional information
?
-
assay substrate is commercial yeast RNA, rates of release of GMP and cGMP show base specificity of enzyme mutant D19N/D22N/E25Q/D31N/D38N/E50Q/E57Q/E76Q/D77N/D79N/E92Q/D93N
-
-
?
additional information
?
-
-
assay substrate is commercial yeast RNA, rates of release of GMP and cGMP show base specificity of enzyme mutant D19N/D22N/E25Q/D31N/D38N/E50Q/E57Q/E76Q/D77N/D79N/E92Q/D93N
-
-
?
additional information
?
-
assay substrate is commercial yeast RNA, the enzyme is a guanylic acid-specific RNase
-
-
?
additional information
?
-
-
assay substrate is commercial yeast RNA, the enzyme is a guanylic acid-specific RNase
-
-
?
additional information
?
-
the enzyme is a guanylic acid-specific ribonuclease
-
-
?
additional information
?
-
-
the enzyme is a guanylic acid-specific ribonuclease
-
-
?
