Cloned (Comment) | Organism |
---|---|
expression of the enzyme in Trp-auxotrophic Escherichia coli, resulting in site-specifically replacement of Trp residues with (2,7-aza)Trp | Aspergillus oryzae |
Crystallization (Comment) | Organism |
---|---|
RNase T1 crystal structure analysis, PDB ID 9RNT | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus oryzae | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
the enzyme can be reversibly unfolded and refolded by heating and high concentration of denaturants, such as urea and guanidine hydrochloride, without forming noticeable amounts of aggregates | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme cleaves single-stranded RNA by catalyzing the hydrolysis of phosphodiester bonds specifically at the 3'-side of guanosine nucleotides | Aspergillus oryzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the secondary structure of RNase T1 includes a long alpha-helix and two beta-sheets connected by extended loop regions, structure of hydrated RNase T1 in loop-close and loop-open forms, overview | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
ribonuclease T1 | - |
Aspergillus oryzae |
RNase T1 | - |
Aspergillus oryzae |
General Information | Comment | Organism |
---|---|---|
additional information | Trp59 may play an important role in folding as well as in modulating the geometry of the RNase T1 active site. Trp59-water pairs appear to preferentially participate in a hydrogen bond network incorporating polar amino acid moieties on the protein surface and bulk waters, providing the structural dynamic features of the connecting loop region in RNase T1, molecular dynamic simulations, overview | Aspergillus oryzae |