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Literature summary for 4.6.1.24 extracted from
- Addressing the chalange of changing the specificity of RNase T1 with rational and evolutionary approaches (2004), ChemBioChem, 5, 200-205.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E46N | mutant shows an improved ApC/GpC preference with a 1450fold increase in comparison to wild-type activity | Aspergillus oryzae |
| Y45W/E46N | mutant shows an improved ApC/GpC preference with a 2100fold increase in comparison to wild-type activity | Aspergillus oryzae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| RNase T1 variants E46N and Y45W/E46N | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenylyl-3',5'-cytidine + H2O | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae | adenosine 3'-phosphate + cytidine | - |
? |  |
| guanylyl-3',5'-cytidine + H2O | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae | ? | - |
? | |
| RNA + H2O | - |
Aspergillus oryzae | Hydrolyzed RNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase T1 | - |
Aspergillus oryzae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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