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4.6.1.24: ribonuclease T1

This is an abbreviated version!
For detailed information about ribonuclease T1, go to the full flat file.

Word Map on EC 4.6.1.24

Reaction

[RNA fragment]-3'-guanosine-2',3'-cyclophosphate
+
H2O
=
[RNA fragment]-3'-guanosine-3'-phosphate

Synonyms

Aspergillus oryzae ribonuclease, barnase, Binase, EC 2.7.7.26, EC 3.1.27.3, EC 3.1.4.8, Gln25-RNase T1, guanyl-preferring RNase, Guanyl-specific RNase, guanylate endoribonuclease, Guanyloribonuclease, More, MycRne, Nuclease, guanyloribo-, Nuclease, ribo-, Aspergillus oryzae, ribonuclease, ribonuclease A, Ribonuclease C2, Ribonuclease Ch, ribonuclease E/G, Ribonuclease F1, ribonuclease G, Ribonuclease guaninenucleotido-2'-transferase (cyclizing), Ribonuclease N1, Ribonuclease N3, ribonuclease NT, ribonuclease Pb2, Ribonuclease PP1, Ribonuclease SA, ribonuclease Sa2, ribonuclease T1, Ribonuclease U1, RNase A, RNase E/G, RNase F1, RNase Fl1, RNase Fl2, RNase G, RNase He1, RNase Ms, RNase N1, RNase N2, RNase NT, RNase Pb1, RNase Pb2, RNase Pc, RNase Po1, RNase Pol, RNase Sa, RNase Sa2, RNase Sa3, RNase St, RNase T1, RNase T1 RV, RNase T1-R2, RNase Th1, RNase U1, Slr1129

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.24 ribonuclease T1

Cloned

Cloned on EC 4.6.1.24 - ribonuclease T1

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a 1.8 kb DNA fragment, encoding the C-terminal portion of the MycRne polypeptide, residues 332-953, is cloned into p6HisF-11d for expression in Escherichia coli BL21-CodonPlusDE3-RIL cells
-
chemically synthesized gene, mutant Gln58Asp, expressed in Escherichia coli host
-
DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)pLysS
DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) pLysS
expression in Epicurian coli
-
expression in Escherichia coli
expression in Escherichia coli ATCC 23231
expression in Escherichia coli BL21 carrying plasmid pGEMGX1/ent/Bi as a homogeneous protein
expression in Saccharomyces cerevisiae
-
expression of the enzyme in Trp-auxotrophic Escherichia coli, resulting in site-specifically replacement of Trp residues with (2,7-aza)Trp
-
for expression in Escherichia coli BL21DE3-Gold cells
-
fusion of the antiferritin antibody VL domain to barnase and expression in Escherichia coli
-
gene rng, overexpression of wild-type rng and of mutant rngs in rne-1 or rneD1018 alleles mutant strains
-
genetic organization, low level expression of barnase by recombinant Bacillus subtilis strains bearing phoPR-null mutations
-
genetic organization, production of binase by recombinant Bacillus subtilis strains bearing phoPR-null mutations is impossible
-
into the pGEM-T vector for transformation of Escherichia coli JM109 cells, into pPIC9K for expression in Pichia pastoris
-
into the pQE30 vector for expression in Escherichia coli M15 cells
-
overexpression in Neurospora crassa, different promoters are tested, the most promising promoter for recombinant expression is the cfp promoter
overexpression in Saccharomyces cerevisiae. Characterization of an rns4/vps32 mutation in the RNase T1 expression-sensitive strain of Saccharomyces cerevisiae. The rns4 mutant is sensitive to both RNase T1 and ambient stress. In contrast to the wild-type strain in which the inactivated RNase T1-GFP fusion protein is localized at the vacuole only under cold stress or nitrogen starvation, the inactivated RNase T1-GFP fusion protein expressed in the rns4 mutant is localized at the ER and vacuole, both under normal growth conditions and upon ambient stress conditions
-
recombinant enzyme expression in Escherichia coli strain BL21(DE3) pLysS
the secondary structure unit mutant S2354 is expressed in Escherichia coli only when His102 is substituted by alanine (H102A)
-