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4.6.1.24: ribonuclease T1

This is an abbreviated version!
For detailed information about ribonuclease T1, go to the full flat file.

Word Map on EC 4.6.1.24

Reaction

[RNA fragment]-3'-guanosine-2',3'-cyclophosphate
+
H2O
=
[RNA fragment]-3'-guanosine-3'-phosphate

Synonyms

Aspergillus oryzae ribonuclease, barnase, Binase, EC 2.7.7.26, EC 3.1.27.3, EC 3.1.4.8, Gln25-RNase T1, guanyl-preferring RNase, Guanyl-specific RNase, guanylate endoribonuclease, Guanyloribonuclease, More, MycRne, Nuclease, guanyloribo-, Nuclease, ribo-, Aspergillus oryzae, ribonuclease, ribonuclease A, Ribonuclease C2, Ribonuclease Ch, ribonuclease E/G, Ribonuclease F1, ribonuclease G, Ribonuclease guaninenucleotido-2'-transferase (cyclizing), Ribonuclease N1, Ribonuclease N3, ribonuclease NT, ribonuclease Pb2, Ribonuclease PP1, Ribonuclease SA, ribonuclease Sa2, ribonuclease T1, Ribonuclease U1, RNase A, RNase E/G, RNase F1, RNase Fl1, RNase Fl2, RNase G, RNase He1, RNase Ms, RNase N1, RNase N2, RNase NT, RNase Pb1, RNase Pb2, RNase Pc, RNase Po1, RNase Pol, RNase Sa, RNase Sa2, RNase Sa3, RNase St, RNase T1, RNase T1 RV, RNase T1-R2, RNase Th1, RNase U1, Slr1129

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.24 ribonuclease T1

General Stability

General Stability on EC 4.6.1.24 - ribonuclease T1

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Conformational stability
-
Guanidine hydrochloride, pH-dependence of denaturation
-
Immobilized enzyme, heat- and pH-stability
-
Insolubilized enzyme
-
MgCl2 increases conformational stability
-
Na2HPO4 increases conformational stability
-
NaCl and low concentrations of guanidinium chloride stabilize RNase Sa, conformational stability is studied by NMR-monitored hydrogen exchange
NaCl, protects from thermal denaturation, increases transition temperature, suppresses unfolding of enzyme by a denaturant, urea, increases conformational stability
-
Proteases, fairly resistant to proteases: carboxypeptidase A, leucine aminopeptidase, trypsin, chymotrypsin <1>, resistant to trypsin and chymotrypsin, sensitive to pepsin
-
Spermine stabilizes
-
the enzyme is remarkably stable at high salt concentrations
-
Urea, 8 M, stable
-
Urea, pH-dependence of urea denaturation
-