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4.6.1.24: ribonuclease T1

This is an abbreviated version!
For detailed information about ribonuclease T1, go to the full flat file.

Word Map on EC 4.6.1.24

Reaction

[RNA fragment]-3'-guanosine-2',3'-cyclophosphate
+
H2O
=
[RNA fragment]-3'-guanosine-3'-phosphate

Synonyms

Aspergillus oryzae ribonuclease, barnase, Binase, EC 2.7.7.26, EC 3.1.27.3, EC 3.1.4.8, Gln25-RNase T1, guanyl-preferring RNase, Guanyl-specific RNase, guanylate endoribonuclease, Guanyloribonuclease, More, MycRne, Nuclease, guanyloribo-, Nuclease, ribo-, Aspergillus oryzae, ribonuclease, ribonuclease A, Ribonuclease C2, Ribonuclease Ch, ribonuclease E/G, Ribonuclease F1, ribonuclease G, Ribonuclease guaninenucleotido-2'-transferase (cyclizing), Ribonuclease N1, Ribonuclease N3, ribonuclease NT, ribonuclease Pb2, Ribonuclease PP1, Ribonuclease SA, ribonuclease Sa2, ribonuclease T1, Ribonuclease U1, RNase A, RNase E/G, RNase F1, RNase Fl1, RNase Fl2, RNase G, RNase He1, RNase Ms, RNase N1, RNase N2, RNase NT, RNase Pb1, RNase Pb2, RNase Pc, RNase Po1, RNase Pol, RNase Sa, RNase Sa2, RNase Sa3, RNase St, RNase T1, RNase T1 RV, RNase T1-R2, RNase Th1, RNase U1, Slr1129

ECTree

     4 Lyases
         4.6 Phosphorus-oxygen lyases
             4.6.1 Phosphorus-oxygen lyases (only sub-subclass identified to date)
                4.6.1.24 ribonuclease T1

Crystallization

Crystallization on EC 4.6.1.24 - ribonuclease T1

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Aspergillus oryzae
-
carboxymethylated RNase T1 crystal structure analysis, PDB ID 1DET
-
crystal structure of RNase T1-T2 (K41E/Y42F/N43R/Y45W/E46N/W59Y), hanging drop vapor diffusion method, 2.1 A resolution
hanging drop vapor diffusion method, crystallization of RNase T1 variant RV (mutant RNase T1 K41E/Y42F/N43R/Y45W/E46N)
RNase T1 crystal structure analysis, PDB ID 9RNT
-
two crystal forms obtained, the classic crystal form I, and the crystals of form II, obtained by repeated seeding
-
the X-ray coordinates of barnase for this study are obtained from the Protein Data Bank, PDB code 1rnb
-
Streptomyces aureofaciens
-
three-dimensional X-ray crystal structure of RNase He1 in complex with Zn
hanging-drop vapor diffusion
mutant enzyme Q94K, hanging-drop vapor-diffusion method at 18°C
RNase Sa-3'IMP complex
structures of two crystal forms (I and II) of ribonuclease Sa2 are presented at 1.8 A and 1.5 A resolution. Vapour diffusion from a solution of 1.0% protein by weight in 0.1 M phosphate buffer at pH 7.2 and room temperature with 40% saturated ammonium sulfate as precipitant
-
Neurospora crassa RNase N1
-
the crystal structures of RNase NT in complex with either 5'-AMP, 5'-GMP, or 2'-UMP are determined at 1.8 A resolutions by molecular replacement
-
purified recombinant enzyme, hanging drop vapor diffusion method, mixing of 500 nl of 10 mg/mL protein in 20 mM Tris-HCl, pH 7.5, with 500 nl of reservoir solution containing 4 M sodium formate, 0.1 M Bis-Tris propane, pH 7.0, 10% PEG 400, and 300 nl 2 M caesium chloride, 20°C, 3 days, X-ray diffraction structure determination and analysis at 1.85 A resolution, molecular replacement method