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Literature summary for 4.6.1.24 extracted from
- Microbial ribonucleases with special reference to RNases T1, T2, N1, and U2 (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 4, 205-250.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| Proteases, fairly resistant to proteases: carboxypeptidase A, leucine aminopeptidase, trypsin, chymotrypsin <1>, resistant to trypsin and chymotrypsin, sensitive to pepsin | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Aspergillus oryzae |  |
| Cu2+ | - |
Aspergillus oryzae | |
| Fe2+ | - |
Aspergillus oryzae | |
| Glyoxal | - |
Aspergillus oryzae | |
| Hg2+ | - |
Aspergillus oryzae | |
| iodoacetate | - |
Aspergillus oryzae | |
| Mg2+ | - |
Aspergillus oryzae | |
| Mn2+ | - |
Aspergillus oryzae | |
| Mononucleotides | e.g. 2'-GMP, 3'-GMP, 5'-GMP, 3'-CMP, 2'(3')-UMP | Aspergillus oryzae | |
| additional information | fairly resistant to proteases, e.g. carboxypeptidase A, leucine aminopeptidase, trypsin, chymotrypsin | Aspergillus oryzae | |
| Na2S | - |
Aspergillus oryzae | |
| Phenylglyoxal | - |
Aspergillus oryzae | |
| substrate analogs | - |
Aspergillus oryzae | |
| Zn2+ | - |
Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH-dependence of Km | Aspergillus oryzae | |
| 0.0238 | - |
GpU | - |
Aspergillus oryzae | |
| 0.0268 | - |
GpA | - |
Aspergillus oryzae | |
| 0.0291 | - |
GpG | - |
Aspergillus oryzae | |
| 0.9445 | - |
GpC | - |
Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 11085 | - |
primary structure, Aspergillus oryzae, 1 * 11085 | Aspergillus oryzae |
| 11090 | - |
Aspergillus oryzae, amino acid analysis | Aspergillus oryzae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| RNA + H2O | Aspergillus oryzae | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| Photooxidation, in presence of Methylene Blue, riboflavine or Rose Bengal inactivation | Aspergillus oryzae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] | mechanism | Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | Taka-diastase, commercial product of Aspergillus oryzae | Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GpA + H2O | - |
Aspergillus oryzae | guanosine 3'-phosphate + adenosine | - |
? | |
| GpG + H2O | - |
Aspergillus oryzae | guanosine 3'-phosphate + guanosine | - |
? | |
| guanosine 2',3'-cyclic phosphate + H2O | - |
Aspergillus oryzae | guanosine-3'-phosphate | - |
? | |
| additional information | synthesis of guanylylnucleosides, oligoguanylate, guanosine-containing oligonucleotides with (3'-5')phosphodiester bonds, synthetic reaction can lead to the formation of unnatural (2'-5')-phosphodiester bonds, not: DNA, L-guanosine 2',3'-cyclic phosphate, L-inosine 2',3'-cyclic phosphate | Aspergillus oryzae | ? | - |
? | |
| RNA + H2O | essential requirements: keto group at 6 position, trivalent nitrogen at 7 position of purine base | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| RNA + H2O | first step (r): cleavage of internucleotide bonds between 3'-guanylic acid groups and the 5'-hydroxyl groups of the adjacent nucleotides with the intermediary formation of guanosine 2',3'-cyclic phosphate, second step (ir): hydrolysis of cyclic phosphate to produce 3'-guanylate | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| RNA + H2O | specificity | Aspergillus oryzae | Hydrolyzed RNA | 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp | ? | |
| RNA + H2O | two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates | Aspergillus oryzae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | primary structure, Aspergillus oryzae, 1 * 11085 | Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
10 min, pH 6 | Aspergillus oryzae |
| 100 | - |
above pH 9 unstable | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
xanthosine 2',3'-cyclic phosphate | Aspergillus oryzae |
| 7.2 | - |
hydrolysis of guanosine 2',3'-cyclic phosphate | Aspergillus oryzae |
| 7.5 | - |
RNA digestion | Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8.5 | - |
Aspergillus oryzae |
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Release 2023.1 (January 2023)
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